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1. US20130053258 - COMBINATORIAL LIBRARY, A METHOD FOR PREPARATION OF THAT COMBINATORIAL LIBRARY, A METHOD FOR SEQUENCE IDENTIFICATION, A METHOD FOR SEQUENCING THE ELEMENTS OF COMBINATORIAL LIBRARIES OF OLIGONUCLEOTIDES AND/OR OLIGONUCLEOTIDE ANALOGUES, THE USE OF A LINKER TO GENERATE COMBINATORIAL LIBRARIES AND A SEQUENCE IDENTIFICATION SET

Office
États-Unis d'Amérique
Numéro de la demande 13636524
Date de la demande 21.03.2011
Numéro de publication 20130053258
Date de publication 28.02.2013
Type de publication A1
CIB
C40B 40/06
CCHIMIE; MÉTALLURGIE
40TECHNOLOGIE COMBINATOIRE
BCHIMIE COMBINATOIRE; BIBLIOTHÈQUES, p.ex. CHIMIOTHÈQUES, BIBLIOTHÈQUES VIRTUELLES (IN SILICO)
40Bibliothèques en soi, p.ex. matrices, mélanges
04Bibliothèques comprenant uniquement des composés organiques
06Bibliothèques comprenant des nucléotides ou des polynucléotides ou leurs dérivés
C40B 20/08
CCHIMIE; MÉTALLURGIE
40TECHNOLOGIE COMBINATOIRE
BCHIMIE COMBINATOIRE; BIBLIOTHÈQUES, p.ex. CHIMIOTHÈQUES, BIBLIOTHÈQUES VIRTUELLES (IN SILICO)
20Procédés spécialement adaptés à l'identification des éléments d'une bibliothèque
08Analyse directe des éléments en soi d'une bibliothèque par des procédés physiques, p.ex. par spectroscopie
C40B 50/14
CCHIMIE; MÉTALLURGIE
40TECHNOLOGIE COMBINATOIRE
BCHIMIE COMBINATOIRE; BIBLIOTHÈQUES, p.ex. CHIMIOTHÈQUES, BIBLIOTHÈQUES VIRTUELLES (IN SILICO)
50Procédés de création de bibliothèques, p.ex. synthèse combinatoire
14Synthèse en phase solide, c. à d. dans laquelle au moins un bloc servant à créer la bibliothèque est lié à un support solide au cours de la création de la bibliothèque; Procédés particuliers de clivage à partir du support solide
CPC
C07H 21/02
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
02with ribosyl as saccharide radical
C12Q 1/6872
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6869Methods for sequencing
6872involving mass spectrometry
Déposants Markiewicz Wojciech T.
Rulka Anna
Inventeurs Markiewicz Wojciech T.
Rulka Anna
Données relatives à la priorité 390797 22.03.2010 PL
Titre
(EN) COMBINATORIAL LIBRARY, A METHOD FOR PREPARATION OF THAT COMBINATORIAL LIBRARY, A METHOD FOR SEQUENCE IDENTIFICATION, A METHOD FOR SEQUENCING THE ELEMENTS OF COMBINATORIAL LIBRARIES OF OLIGONUCLEOTIDES AND/OR OLIGONUCLEOTIDE ANALOGUES, THE USE OF A LINKER TO GENERATE COMBINATORIAL LIBRARIES AND A SEQUENCE IDENTIFICATION SET
Abrégé
(EN)

The invention provides a combinatorial library, a method for preparation of that combinatorial library, a method for sequence identification, a method for sequencing the elements of combinatorial libraries of oligonucleotides and/or oligonucleotide analogues, the use of a linker to generate combinatorial libraries and a sequence identification set. More precisely, the objective of the invention is the ability to “read” sequences of selected elements of combinatorial libraries of freely modified synthetic oligonucleotides. The solution may be used both for researching for leading compounds in the pharmaceutical industry, and as a tool for studying the properties of oligonucleotides in the aspect of their potential use in experimental antisense or antigen therapy. The invention develops an appropriate strategy for determining the structure of isolated library elements, as usefulness of the combinatorial library depends on the ability to recognize the structure of its elements. Thanks to the developed and presented method for sequencing combinatorial oligonucleotide libraries it is possible to indisputably identify the sequences of biologically active elements selected by the combinatorial synthesis method.


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