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1. JP2020122656 - IMMUNOCHROMATOGRAPHIC TEST PIECE FOR EXTRACTING AND MEASURING CARBOHYDRATE ANTIGEN WITH WHICH SPECIMEN DEVELOPMENT CAN BE CONTROLLED BY IMPREGNATING NITRITE SALT OR SOLID ACID REAGENT IN HYDROPHILIC MATERIAL

Office
Japon
Numéro de la demande 2019012835
Date de la demande 29.01.2019
Numéro de publication 2020122656
Date de publication 13.08.2020
Type de publication A
CIB
G01N 33/543
GPHYSIQUE
01MÉTROLOGIE; TESTS
NRECHERCHE OU ANALYSE DES MATÉRIAUX PAR DÉTERMINATION DE LEURS PROPRIÉTÉS CHIMIQUES OU PHYSIQUES
33Recherche ou analyse des matériaux par des méthodes spécifiques non couvertes par les groupes G01N1/-G01N31/146
48Matériau biologique, p.ex. sang, urine; Hémocytomètres
50Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique
53Tests immunologiques; Tests faisant intervenir la formation de liaisons biospécifiques; Matériaux à cet effet
543avec un support insoluble pour l'immobilisation de composés immunochimiques
G01N 33/53
GPHYSIQUE
01MÉTROLOGIE; TESTS
NRECHERCHE OU ANALYSE DES MATÉRIAUX PAR DÉTERMINATION DE LEURS PROPRIÉTÉS CHIMIQUES OU PHYSIQUES
33Recherche ou analyse des matériaux par des méthodes spécifiques non couvertes par les groupes G01N1/-G01N31/146
48Matériau biologique, p.ex. sang, urine; Hémocytomètres
50Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique
53Tests immunologiques; Tests faisant intervenir la formation de liaisons biospécifiques; Matériaux à cet effet
G01N 33/569
GPHYSIQUE
01MÉTROLOGIE; TESTS
NRECHERCHE OU ANALYSE DES MATÉRIAUX PAR DÉTERMINATION DE LEURS PROPRIÉTÉS CHIMIQUES OU PHYSIQUES
33Recherche ou analyse des matériaux par des méthodes spécifiques non couvertes par les groupes G01N1/-G01N31/146
48Matériau biologique, p.ex. sang, urine; Hémocytomètres
50Analyse chimique de matériau biologique, p.ex. de sang ou d'urine; Test par des méthodes faisant intervenir la formation de liaisons biospécifiques par ligands; Test immunologique
53Tests immunologiques; Tests faisant intervenir la formation de liaisons biospécifiques; Matériaux à cet effet
569pour micro-organismes, p.ex. protozoaires, bactéries, virus
CPC
G01N 33/53
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
G01N 33/543
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
G01N 33/569
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
Déposants DENKA SEIKEN CO LTD
デンカ株式会社
Inventeurs KATO DAISUKE
加藤 大介
MURAMATSU SHINO
村松 志野
Mandataires 特許業務法人平木国際特許事務所
Titre
(EN) IMMUNOCHROMATOGRAPHIC TEST PIECE FOR EXTRACTING AND MEASURING CARBOHYDRATE ANTIGEN WITH WHICH SPECIMEN DEVELOPMENT CAN BE CONTROLLED BY IMPREGNATING NITRITE SALT OR SOLID ACID REAGENT IN HYDROPHILIC MATERIAL
(JA) 親水性素材に亜硝酸塩又は固形状酸性試薬を含浸させることにより、検体の展開を制御し得る、糖鎖抗原を抽出し測定するためのイムノクロマト試験片
Abrégé
(EN)

PROBLEM TO BE SOLVED: To provide an immunochromatographic test piece and an immunochromatographic method using the test piece for controlling specimen development on an immunochromatographic test piece and appropriately controlling treatment by an acid reagent, nitrite salt and neutralizing reagent.

SOLUTION: Provided is an immunochromatographic test piece including a sample pad for adding a specimen mixed with nitrite sale or acid solution, a labeled product area that includes a labeled antibody in which an antibody for a carbohydrate antigen is labeled and a detection area in which an antibody for the carbohydrate antigen, for forming an antibody-carbohydrate antigen-labeled antibody composite in the detection area and measuring the carbohydrate antigen. The immunochromatographic test piece includes an area, upstream of the labeled product area, which is impregnated with a neutralizing reagent and further includes an area, upstream of the area impregnated with a neutralizing reagent, which is impregnated with a solid acid reagent when a specimen mixed with nitrite salt is used and an area impregnated with nitrite salt when a specimen mixed with an acid solution is used, for extracting and measuring a carbohydrate antigen in the specimen, with a hydrophilic material used in the area impregnated with the nitrite salt or the solid acid reagent.

SELECTED DRAWING: None

COPYRIGHT: (C)2020,JPO&INPIT

(JA)

【課題】本発明は、イムノクロマト試験片上での検体の展開を制御し、酸性試薬と亜硝酸塩と中和試薬による処理を適切に制御するためのイムノクロマト試験片及び該試験片を用いたイムノクロマト方法を提供することを目的とする。
【解決手段】亜硝酸塩又は酸性溶液と混合した検体を添加するサンプルパッド、糖鎖抗原に対する抗体を標識した標識抗体を含む標識体領域及び前記糖鎖抗原に対する抗体を固相化した検出領域を含み、検出領域において抗体-糖鎖抗原-標識抗体の複合体を形成させ、糖鎖抗原を測定するイムノクロマト試験片であって、前記標識体領域の上流に中和試薬を含浸させた領域を有し、さらに該中和試薬を含浸させた領域の上流に、亜硝酸塩と混合した検体を用いるときには固形状酸性試薬を含浸させた領域を有し、酸性溶液と混合した検体を用いるときには亜硝酸塩を含浸させた領域を有する、検体中の糖鎖抗原を抽出し測定するためのイムノクロマト試験片であって、亜硝酸塩又は固形状酸性試薬を含浸させた領域に親水性を有する素材を用いることを特徴とする、イムノクロマト試験片。
【選択図】なし

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