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1. CN101932933 - Formation of layers of amphiphilic molecules

Office Chine
Numéro de la demande 200880126160.3
Date de la demande 15.12.2008
Numéro de publication 101932933
Date de publication 29.12.2010
Numéro de délivrance 101932933
Date de délivrance 01.01.2014
Type de publication B
CIB
G01N 33/487
GPHYSIQUE
01MÉTROLOGIE; TESTS
NRECHERCHE OU ANALYSE DES MATÉRIAUX PAR DÉTERMINATION DE LEURS PROPRIÉTÉS CHIMIQUES OU PHYSIQUES
33Recherche ou analyse des matériaux par des méthodes spécifiques non couvertes par les groupes G01N1/-G01N31/146
48Matériau biologique, p.ex. sang, urine; Hémocytomètres
483Analyse physique de matériau biologique
487de matériau biologique liquide
B01D 67/00
BTECHNIQUES INDUSTRIELLES; TRANSPORTS
01PROCÉDÉS OU APPAREILS PHYSIQUES OU CHIMIQUES EN GÉNÉRAL
DSÉPARATION
67Procédés spécialement adaptés à la fabrication de membranes semi-perméables destinées aux procédés ou aux appareils de séparation
CPC
B01L 3/502707
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware
50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
502with fluid transport, e.g. in multi-compartment structures
5027by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
502707characterised by the manufacture of the container or its components
B01L 2300/0645
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
06Auxiliary integrated devices, integrated components
0627Sensor or part of a sensor is integrated
0645Electrodes
B01L 2300/161
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
2300Additional constructional details
16Surface properties and coatings
161Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
G01N 27/333
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
27Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
26by investigating electrochemical variables; by using electrolysis or electrophoresis
28Electrolytic cell components
30Electrodes, e.g. test electrodes; Half-cells
333Ion-selective electrodes or membranes
G01N 33/48721
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
483Physical analysis of biological material
487of liquid biological material
48707by electrical means
48721Investigating individual macromolecules, e.g. by translocation through nanopores
B01D 67/00
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
67Processes specially adapted for manufacturing semi-permeable membranes for separation processes or apparatus
Déposants 牛津纳米孔技术有限公司
Inventeurs 斯图尔特·威廉·里德
特伦斯·阿兰·里德
詹姆斯·安东尼·克拉克
史蒂文·保罗·怀特
朱迪尔·辛格·桑赫拉
Mandataires 北京三友知识产权代理有限公司 11127
北京三友知识产权代理有限公司 11127
Données relatives à la priorité 0724736.4 19.12.2007 GB
61/080,492 14.07.2008 US
Titre
(EN) Formation of layers of amphiphilic molecules
(ZH) 两性分子层的形成
Abrégé
(EN)
To form a layer (11) separating two volumes of aqueous solution, especially a biolayer lipid membrane (BLM) there is used an apparatus comprising elements defining a chamber (7), the elements including a body (2) of non-conductive material having formed therein at least one recess (5) opening into the chamber, the recess containing an electrode (21). A pre-treatment coating of a hydrophobic fluid is applied to the body across the recess. Aqueous solution, having amphiphilic molecules added thereto, is flowed across the body to cover the recess so that aqueous solution is introduced into the recess from the chamber and a layer of the amphiphilic molecules forms across the recess separating a volume of aqueous solution introduced into the recess from the remaining volume of aqueous solution.

(ZH)

为了形成分隔两个体积水溶液的层(11),尤其是生物层脂质膜(BLM),使用了包含限定腔室(7)的元件的装置,所述元件包括其中形成有至少一个通向所述腔室的凹槽(5)的非导电性材料的主体(2),所述凹槽包含电极(21)。在跨越所述凹槽的所述主体上涂布疏水性流体预处理涂料。其中添加有两性分子的水溶液流过所述主体而覆盖所述凹槽,从而使水溶液由所述腔室导入所述凹槽,两性分子层跨越所述凹槽形成,并且将导入所述凹槽中的水溶液的体积与水溶液的其余体积隔开。