(EN)
The invention relates to a method for expressing cecropin CAD antibacterial peptide by means of a recombined bacillus subtilis expression system. Through carrying out artificial synthesis of sumo protease expression operon, saccharomyces cerevisiae micromolecular ubiquitin-like protein and cecropin AD fused protein expression operon gene, the method clones cecropin AD matrix gene bacillus subtilis expression vector pNF11 and converts recombined expression vector pNF 11-CAD, thereby ensuring the induced expression of recombined bacillus subtilis in a shake flask. The method has the advantages of a simple expression system, large-scale production, low production cost, strong biological activity and no toxic and harmful substance, etc; moreover, the method provides a medicine with low price and strong antibacterial capacity for clinic disease prevention and cure, and the medicine can also be used as feedstuff additive.
(ZH) 本发明涉及一种以重组枯草杆菌表达系统表达天蚕素CAD抗菌肽的方法,通过人工合成sumo蛋白酶的表达操纵子、酿酒酵母小分子泛素样蛋白与天蚕素AD的融合蛋白表达操纵子基因,克隆天蚕素AD母体基因枯草杆菌表达载体pNF11,转化重组表达载体pNF 11-CAD,从而使重组枯草杆菌在摇瓶中诱导表达;本发明具有表达系统简单,用于大规模生产,生产成本低,生物活性强,无毒害物质等优点;为临床上的疾病防治和治疗提供一种价格便宜、抗菌力强的药物,也可作为饲料添加剂。