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1. BRPI0212145 - Isolamento de proteìnas envolvidas no silenciamento gênico pós-transcricional e métodos de uso

Office
Brésil
Numéro de la demande 212145-0
Date de la demande 26.08.2002
Numéro de publication PI0212145
Date de publication 16.08.2005
Type de publication A
CIB
C12N 9/22
CCHIMIE; MÉTALLURGIE
12BIOCHIMIE; BIÈRE; SPIRITUEUX; VIN; VINAIGRE; MICROBIOLOGIE; ENZYMOLOGIE; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE
NMICRO-ORGANISMES OU ENZYMES; COMPOSITIONS LES CONTENANT; CULTURE OU CONSERVATION DE MICRO-ORGANISMES; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE; MILIEUX DE CULTURE
9Enzymes, p.ex. ligases (6.); Proenzymes; Compositions les contenant; Procédés pour préparer, activer, inhiber, séparer ou purifier des enzymes
14Hydrolases (3.)
16agissant sur les liaisons esters (3.1)
22Ribonucléases
C12N 15/29
CCHIMIE; MÉTALLURGIE
12BIOCHIMIE; BIÈRE; SPIRITUEUX; VIN; VINAIGRE; MICROBIOLOGIE; ENZYMOLOGIE; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE
NMICRO-ORGANISMES OU ENZYMES; COMPOSITIONS LES CONTENANT; CULTURE OU CONSERVATION DE MICRO-ORGANISMES; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE; MILIEUX DE CULTURE
15Techniques de mutation ou génie génétique; ADN ou ARN concernant le génie génétique, vecteurs, p.ex. plasmides, ou leur isolement, leur préparation ou leur purification; Utilisation d'hôtes pour ceux-ci
09Technologie d'ADN recombinant
11Fragments d'ADN ou d'ARN; Leurs formes modifiées
29Gènes codant pour des protéines végétales, p.ex. thaumatine
C12N 15/82
CCHIMIE; MÉTALLURGIE
12BIOCHIMIE; BIÈRE; SPIRITUEUX; VIN; VINAIGRE; MICROBIOLOGIE; ENZYMOLOGIE; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE
NMICRO-ORGANISMES OU ENZYMES; COMPOSITIONS LES CONTENANT; CULTURE OU CONSERVATION DE MICRO-ORGANISMES; TECHNIQUES DE MUTATION OU DE GÉNÉTIQUE; MILIEUX DE CULTURE
15Techniques de mutation ou génie génétique; ADN ou ARN concernant le génie génétique, vecteurs, p.ex. plasmides, ou leur isolement, leur préparation ou leur purification; Utilisation d'hôtes pour ceux-ci
09Technologie d'ADN recombinant
63Introduction de matériel génétique étranger utilisant des vecteurs; Vecteurs; Utilisation d'hôtes pour ceux-ci; Régulation de l'expression
79Vecteurs ou systèmes d'expression spécialement adaptés aux hôtes eucaryotes
82pour cellules végétales
CPC
C12N 9/22
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
16acting on ester bonds (3.1)
22Ribonucleases ; RNAses, DNAses
C12N 15/8218
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
C12N 15/8283
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8261with agronomic (input) traits, e.g. crop yield
8271for stress resistance, e.g. heavy metal resistance
8279for biotic stress resistance, pathogen resistance, disease resistance
8283for virus resistance
Déposants TEXAS A & M UNIV SYS
Inventeurs MIRKOV ERIK T
INGELBRECHT IVAN L
Données relatives à la priorité 02/27300 26.08.2002 US
10226715 23.08.2002 US
60/314,863 24.08.2001 US
Titre
(PT) Isolamento de proteìnas envolvidas no silenciamento gênico pós-transcricional e métodos de uso
Abrégé
(PT)
"ISOLAMENTO DE PROTEìNAS ENVOLVIDAS NO SILENCIAMENTO GêNICO PóS-TRANSCRICIONAL E MéTODOS DE USO". A presente invenção inclui um método para a detecção e isolamento de proteínas da cana-de-açúcar que interagem com as proteínas HC-Pro e P1 da SRMV e com outras proteìnas envolvidas no silenciamento de genes, particularmente na cana-de-açúcar. O método utiliza um ensaio duplo híbrido como uma HC-Pro, P1, ou outra proteína relacionada ao silenciamento, contendo uma proteína isca e uma proteína presa contendo um polipeptídio codificado por uma molécula de DNA em uma biblioteca CDNA. O método também inclui a identificação de falsos positivos através de ensaios duplo híbrido reversos e utilizando técnicas e in vitro tais como o farwestern blot ou prestes destrutivos nos quais as condições fisiológicas da planta podem ser replicadas. Finalmente, as interações podem ser confirmadas na planta. Algumas novas proteínas utilizadas aqui e descobertas utilizando esses métodos também são identificadas. Também são discutidos métodos para regular o silenciamento em plantas tais como a cana-de-açúcar utilizando proteínas virais e de planta.