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1. AU2001039534 - Single chain class I major histo-compatibility complexes, constructs encoding same and methods of generating same

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SINGLE CHAIN CLASS I MAJOR HISTO-COMPATIBILITY COMPLEXES, CONSTRUCTS ENCODING SAME AND METHODS
OF GENERATING SAME
FIELD AND BACKGROUND OF THE INVENTION
The present invention relates to methods of generating a functional mammalian single chain MHC class I complex in prokaryotic expression systems and a functional human single chain MHC class I complex in eukaryotic or prokaryotic expression systems, which complexes are capable of presenting specific antigenic peptides restricted to class I MI-IC and recognizable by specific CTL clones or CD+8 T-cells. The present invention further relates to a method of generating a functional mammalian single chain MHC class I-peptide complex in eukaryotic, or preferably prokaryotic expression systems, to nucleic acid constructs encoding said single chain MHC class I complexes and to a novel human single chain MHC class I polypeptide.
The major histocompatibility complex (MHC) is a complex of antigens encoded by a group of linked loci, which are collectively termed H-2 in the mouse and HLA in humans. The two principal classes of the MHC antigens, class I and class II, each comprise a set of cell surface glycoproteins which play a role in determining tissue type and transplant compatibility. In transplantation reactions, cytotoxic T-cells (CTLs) respond mainly against foreign class I glycoproteins, while helper T-cells respond mainly against foreign class II glycoproteins.
Major histocompatibility complex (MHC) class I molecules are expressed on the surface of nearly all cells. These molecules function in presenting peptides which are mainly derived from endogenously synthesized proteins to CD8+ T cells via an interaction with the ct T-cell receptor The class I MHC molecule is a heterodimer composed of a 46-kDa heavy chain which is non-covalently associated with the 12-kDa light chain 3-2 microglobulin. Class I MHC-restricted peptides, which are typically 8-10-amino acid-long, bind to the heavy chain a(l-ca2 groove via two or three anchor residues that interact with corresponding binding pockets in the MHC molecule. The p-2 microglobulin chain plays an important role in MHC class I intracellular transport, peptide binding, and conformational stability For most class I molecules, the formation of a heterodimer consisting of the MHC class I heavy chain, peptide (self or antigenic) and p-2 microglobulin is required for biosynthetic maturation and cell-surface expression Research studies performed on peptide binding to class I MHC molecules enable to define specific MHC motifs functional in displaying peptides derived from viral or tumor antigens that are potentially immunogenic and might elicit specific response from cytotoxic T lymphocytes (CTLs)
The realization that CTLs have an important role in the control of many diseases, including chronic viral diseases, such as AIDS, and cancer have lead to an increased need to produce sufficient amounts of stable class I MHC complexes for functional and structural studies.
Soluble MHC molecules bound to various peptides are a valuable tool for the study of disease-related immune responses, for characterizing MHC-T-cell receptor (TCR) interactions for structural studies and more recently for direct visualization of antigen-specific T cells These molecules can be also used to activate specific CTLs in vitro as well as to study their phenotypic characteristics.
In recent years, various approaches have been used in attempts to develop an in vitro protocol for the induction of cytotoxic T cell responses against viral and tumor antigens To effectively activate T-cells, a high density of MHC-peptide complexes on the surface of the antigen presenting cells must be utilized Thus, a desirable approach for in-vitro T-cell activation would be to use soluble MHC-peptide complexes.
To overcome the low affinity binding of TCRs to soluble MHC molecules and as such to provide efficient T-cell activation, multimerization of the MHC-peptide complexes must be effected.
Soluble MHC multimers posses a higher avidity for T-cells since they provide multi-point binding of TCRs with their MHC-peptide ligands. As such, multimeric forms (tetramers) of MHC-peptide complexes have been the center of much interest recently, because they can be used for direct phenotypic characterization of T cell responses in normal as well as pathological conditions, thus, providing insight into the pathopysiology and mechanisms of various diseases.
However, such studies require a reproducible method for producing large amounts of soluble and functional multimeric MHC-peptide complexes. Thus, attempts were made to produce recombinant MHC class I and class II complexes [11-23] which are soluble and which can be produced in large quantities.
Early studies utilizing recombinant techniques, separately expressed the heavy chain and P-2 microglobulin components of the MHC complex in E. Coli and subsequently refolded them in-vitro in the presence of an antigenic peptide [11].
More recently, recombinant MHC complexes were expressed in eukaryotic expression systems and secreted therefrom in the form of a single polypeptide which included the heavy chain covalently linked to the P-2 microglobulin chain thus forming a stable and functional MHC complex which can be subsequently bound to a peptide of interest [12-15,19,21-23]. The expression of functional MHC complexes in eukaryotic cells suffers from several inherent limitations. Since the expressed polypeptides form a functional MHC complex they bind peptides endogenously derived from the cells utilized for expression and as such the purified MHC complex must be subjected to a peptide exchange step following purification In addition, the production yields of these single-chain MHC-peptide complexes was limited, typically reaching levels of several hundred micrograms per liter of culture supernatant.
The present invention provides a novel approach for the production of unprecedented large amounts of soluble, stable and functional MHC-peptide complex by utilizing high level bacterial expression of a single-chain MHC class I polypeptide or co-expression of the single chain MHC class I polypeptide and an MHC class I restricted antigenic peptide followed by in-vitro reconstitution of the scMHC class I-peptide complex via redox-shuffling and refolding in the presence of the antigenic peptide.
The present invention further provides a novel human single chain MHC class I polypeptide which is functional and which can therefore be utilized in either the monomeric or preferably the multimeric form to present MHC class 1 restricted antigenic peptides to CTL clones or to CD8+ T-cells from various sources.
SUMMARY OF THE INVENTION
According to one aspect of the present invention there is provided a nucleic acid construct comprising a first nucleic acid sequence including a first polynucleotide encoding a functional human P-microglobulin, being translationally fused upstream of a second polynucleotide encoding a functional human MHC class I heavy chain. According to further features in preferred embodiments of the invention described below, the nucleic acid construct further comprising a second nucleic acid sequence encoding an antigenic peptide, the antigenic peptide being capable of binding a human MHC class I complex.
According to still further features in the described preferred embodiments the second polynucleotide encodes ct 1-3 domains of the human MHC class I heavy chain.
According to another aspect of the present invention there is provided a nucleic acid construct comprising a first nucleic acid sequence including a first polynucleotide encoding a functional mammalian P-2 microglobulin; and (ii) a second polynucleotide encoding a functional mammalian MHC class I heavy chain, the second polynucleotide being translationally fused downstream of the first polynucleotide; and a cis acting regulatory sequence being selected capable for directing expression of the first nucleic acid sequence in bacteria.
According to still further features in the described preferred embodiments the cis acting regulatory sequence is selected from the group consisting of a bacterial derived cis acting regulatory sequence and a phage derived cis acting regulatory sequence.
According to still another aspect of the present invention there is provided a transformed cell comprising any of the nucleic acid constructs described herein. The cell can be a eukaryotic cell, such as, for example, a mammalian cell, an insect cell, a plant cell, a yeast cell and a protozoa cell, or alternatively, the cell can be a bacterial cell.
According to still another aspect of the present invention there is provided a host cell being co-transformed with a first expression construct including a first polynucleotide encoding a functional mammalian P-2 microglobulin, being translationally fused upstream of a second polynucleotide encoding a functional MHC class I heavy chain; and a second expression construct including a third polynucleotide encoding an antigenic peptide, wherein when the first, second and third polynucleotides are co-expressed in the host cell, an MHC class I-antigenic peptide complex is formed.
According to still further features in the described preferred embodiments the first nucleic acid sequence further includes an in-frame linker polynucleotide encoding a linker peptide interposed between the first and the second polynucleotides.
According to still further features in the described preferred embodiments the first nucleic acid sequence further includes an in-frame tag sequence encoding a peptide capable of being enzymatically modified to include a binding entity.
According to still further features in the described preferred embodiments the linker peptide is as set forth in SEQ ID
According to still further features in the described preferred embodiments the nucleic acid construct further comprising a first cis acting regulatory sequence for regulating expression of the first nucleic acid sequence.
According to still further features in the described preferred embodiments the nucleic acid construct further comprising a second cis acting regulatory sequence for regulating expression of the second nucleic acid sequence.
According to still further features in the described preferred embodiments the cis acting regulatory sequence is functional in a bacterial host.
According to still another aspect of the present invention there is provided a recombinant polypeptide comprising an amino acid sequence including a functional human P-2 microglobulin directly or indirectly covalently linked to a functional human MHC class I heavy chain.
According to still further features in the described preferred embodiments the recombinant polypeptide further comprising a linker peptide being interposed between the functional human P-2 microglobulin and the functional human MHC class I heavy chain.
Preferably, the recombinant polypeptide comprising an amino acid sequence as set forth in SEQ ID
According to yet another aspect of the present invention there is provided a preparation of bacterial derived inclusion bodies comprising over 30 percent by weight of a recombinant polypeptide including an amino acid sequence including a functional mammalian P-2 microglobulin directly or indirectly covalently linked to a functional mammalian MHC class I heavy chain.
According to an additional aspect of the present invention there is provided a method of producing a functional MHC class I molecule comprising the steps of expressing, in bacteria, a single chain MHC class I polypeptide including a functional mammalian 0-2 microglobulin amino acid sequence directly or indirectly covalently linked to a functional mammalian MHC class I heavy chain amino acid sequence; and isolating the single chain MHC class I polypeptide.
According to still further features in the described preferred embodiments the method further comprising the step of refolding the single chain MHC class I polypeptide in presence of an antigenic peptide capable of binding the single chain MHC class I polypeptide, to thereby generate an MHC class I-antigenic peptide complex.
According to still further features in the described preferred embodiments the method further comprising the step of isolating the MHC class I-antigenic peptide complex via size exclusion chromatography.
According to still further features in the described preferred embodiments the antigenic peptide is co-expressed along with the single chain MHC class I polypeptide in the bacteria.
According to still further features in the described preferred embodiments step is effected such that the single chain MHC class I polypeptide forms inclusion bodies in the bacteria.
According to still further features in the described preferred embodiments the antigenic peptide and the single chain MHC class I polypeptide form inclusion bodies in the bacteria.
According to still further features in the described preferred embodiments the step of isolating the polypeptide further includes the steps of denaturing the inclusion bodies so as to release protein molecules therefrom; and (ii) renaturing the protein molecules.
According to still further features in the described preferred embodiments the step of renaturing the protein molecules is effected in the presence of an antigenic peptide capable of binding the single chain MHC class I polypeptide.
According to still further features in the described preferred embodiments the antigenic peptide is co-expressed along with the single chain MHC class I polypeptide in the bacteria.
According to still further features in the described preferred embodiments the mammalian 0-2 microglobulin amino acid sequence is a human 0-2 microglobulin amino acid sequence and further wherein the mammalian MHC class I heavy chain amino acid sequence is a human MHC class I heavy chain amino acid sequence.
According to still another aspect of the present invention, there is provided a multimeric MHC class I complex comprising a plurality of recombinant polypeptide monomers each including a functional human P -2 microglobulin directly or indirectly covalently linked to a functional human MHC class I heavy chain.
According to still further features in the described preferred embodiments the plurality of recombinant polypeptide monomers are linked to a common substrate.
The present invention successfully addresses the shortcomings of the presently known configurations by providing a method of generating large quantities of pure single chain MHC class I polypeptides which can be utilized in monomeric or multimeric form to present antigenic peptides to CTL clones. The present invention further addresses the shortcomings of the presently known configurations by providing, for the firs time, a human single chain MHC class I polypeptide functional in both monomeric and multimeric form in presenting antigenic peptides to CTL clones.
Definitions of the specific embodiments of the invention as claimed herein follow.
According to a first embodiment of the invention, there is provided a multimeric MHC class I complex comprising a plurality of recombinant polypeptide monomers each including a functional human 3-2 microglobulin covalently linked upstream ofa functional human MHC class I heavy chain.
According to a second embodiment of the invention, there is provided a i.I: plurality of complexes, each being composed of an antigenic peptide being capable of binding a human MHC class I, and a chimeric polypeptide which comprises a functional human 3-2 microglobulin translationally fused upstream of a functional human MHC class I heavy chain, wherein all of said plurality of complexes are recognizable by one CTL clone.
According to a third embodiment of the invention, there is provided a multimeric MHC class I-peptide complex comprising at least 2 complexes of the plurality of complexes according to the second embodiment.
BRIEF DESCRIPTION OF THE DRAWINGS
The invention is herein described, by way of example only, with reference to the accompanying drawings. With specific reference now to the drawings in detail, it is stressed that the particulars shown are by way of example and for purposes of illustrative discussion of the preferred embodiments of the present invention only, and are presented in the cause of providing what is believed to be the most useful and readily understood description of the principles and conceptual aspects of the invention. In this regard, no attempt is made to show structural details of the invention in more detail than is necessary for a fundamental understanding of the invention, the description taken with the drawings making apparent to those skilled in the art how the several forms of the invention may be embodied in practice.
In the drawings:
FIG. 1 illustrates the various expression cassettes of the present invention which were cloned into a bacterial expression vector. 32-M human P-2 microglobulin, HLA-A2 human MHC class 1 heavy chain, BirA a sequence encoding a peptide including a biotin protein ligase-BirA enzyme recognition sequence.
FIGs 2a-b are SDS page gels depicting the analysis of the expression products of the various constructs of Figure 1. The 3-2 microglobulin, HLA-A2, and the scMHC polypeptides were expressed in E. coli BL21 cells and the peptides accumulated as insoluble
SText    continues        on   page     10.1 
inclusion bodies. Inclusion bodies were purified and analyzed by reduced SDS-PAGE electrophoresis on 10% gels. p-2 human p-2 microglobulin, HLA-A2 human MHC class I heavy, S.C. p2-A2 human scMHC class I and S.C. P2-A2-BirA human scMHC-BirA. Arrows indicate molecular size markers. In all cases, the recombinant polypeptide expressed comprised >90% of total inclusion body protein.
FIGs. 3a-c are SDS-PAGE gels depicting the analysis of refolded purified single-chain MHC-peptide complexes. scMHC-peptide complexes were generated by refolding of purified solubilized inclusion bodies in the presence of antigenic peptides as described in the Examples section. The refolded complexes were further purified by size-exclusion chromatography and fractions were analyzed by non-reduced SDS-PAGE on 10% gels. depicted are representative fractions of the scMHC complexed with the G9-209-2M (Figure 3a) and G9-280-9V (Figure 3b) gpl00-derived peptides and the scMHC-BirA- complexed with the G9-280-9V peptide (Figure 3c).
FIG. 4 is a CD spectra analysis of a refolded single-chain MHC-peptide complex collected and analyzed as described in the Examples section below.
FIGs. 5a-b are graphs depicting antibody analysis of single-chain MHC-peptide complexes. Binding of conformation-specific antibodies to refolded and purified scMHC-peptide complexes was performed by a capture double sandwich ELISA assay as further described in the Examples section. Anti-HLA-A2 specific mAb BB7.2 antibody was used to capture the soluble scMHC-peptide complex and the biotinylated mAb W6/32 specific for fully assembled, peptide-containing HLA-A2 was used for detection (Figure 5a). The reaction was developed using streptavidin-peroxidase conjugate. Specific recognition of the singlechain MHC-BirA-peptide complex by the W6/32 antibody was also determined for biotinylated or unbiotinylated complexes that were immobilized to streptavidin-coated magnetic beads (Figure
FIGs. 6a-c depict mass spectroscopy analysis of single-chain MHC-peptide complexes. The scMHC-209 complex was resolved by HPLC on a C-8 hydrophobic column, and eluted with a linear gradient of acetonitrile in TFA, The sample was microsprayed directly from the HPLC column into an electrospray ion trap (ESI) mass spectrometer (LCQ) and analyzed in the positive ion mode. Figure 6a depicts mass spectrometry analysis of eluted peptides, wherein Figures 6b-c depict mass estimation of the proteins.
FIGs. 7a-c are graphs depicting the biological activity of singlechain MHC-peptide complexes as determined by measuring the release of IFNg from specific CTL clones. Figure 7a depicts the ability of various scMHC-peptide complexes coated onto microtiter plates to activate the G9-209-2M specific CTI, clone R6C12. Spontaneous release (Spont) was determined by incubation of CTLs in wells that were not coated with the various complexes. Figure 7b depicts the role of peptide specificity in CTI activation. scMHC tetramers complexed with the G9-209-2M peptide were generated as described in the Examples section. The soluble tetramers were incubated with the 209-specific CTL clone R6C12 or with the Mart-1-specific clone JB2F4 as indicated. Spontaneous IFNg release was determined by incubating the CTL clones with the unbiotinylated scMHC-peptide complex (which does not support tetramer formation). Figure 7c depicts the dependency of CTLs activation on tetramer formation. The 209-specific CTL clone R6C12 was incubated with various biotinylated or unbiotinylated scMHC-BirA-peptide complexes and streptavidin. Spontaneous release was determined by incubating CTLs with the scMHC-209 complex which does not contain the BirA tag.
FIGs. 8a-e are microscopic images of microtiter plate wells containing the G9-209-2M specific CTL clone R6C12 incubated with biotinylated and thus tetramer forming scMHC-BirA-209 complexes or unbiotinylated scMHC-BirA-209 complexes which are incapable of forming tetramers and which served as controls. Cell agglutination was peptide specific and dependent on the concentration of the scMHC-BirA-209 tetramer (Figure 8b). As the concentration of tetramers increased, increased cell agglutination (rosette formation) was observed (Figure 8c-CTLs incubated with unbiotinylated scMHC-BirA-209 complexes and streptavidin did not exhibit any morphological appearance indicating of cell agglutination (Figure 8a).
FIGs. 9a-b are FACS analysis images of CTL clones specific for the MART-1 peptide 27-35 incubated with either scMHC-BirA/MART-1 PE-labeled tetramers (Figure 9a) or with scMHC-BirA/TAX PE-labeled tetramers (Figure 9b) and co-stained with FITC-labeled anti CD8 antibody. Specific staining of high avidity CTLs was observed only when using the specific and functional MART-1 peptide-containing scMHC tetramer.
FIGs. O1a-b are FACS analysis images of a heterogeneous population of T-cells from an HLA-A2 transgenic mice immunized with the TAX peptide. Figure 10a is the FACS image of T- cells from nonimmunized mice stained with scMHC-BirA/TAX tetramer and Figure is the FACS image of T-cells from TAX-immunized mice stained with the tetramers. Both preparations were also double stained with anti-CD8.
DESCRIPTION OF THE PREFERRED EMBODIMENTS
The present invention is of methods of generating a functional mammalian single chain MHC class I complex in prokaryotic expression systems and a functional human single chain MHC class I complex in eukaryotic or prokaryotic expression systems, which complexes are capable of presenting specific antigenic peptides restricted to specific CTL clones. The present invention is further of a method of generating a functional mammalian single chain MHC class I-peptide complex in eukaryotic, or preferably prokaryotic expression systems. The present invention is also of nucleic acid constructs encoding said single chain MHC class I complexes and of a novel human single chain MHC class I polypeptide.
The principles and operation of the present invention may be better understood with reference to the accompanying descriptions.
Before explaining at least one embodiment of the invention in detail, it is to be understood that the invention is not limited in its application to the details of construction and the arrangement of the components set forth in the following description or illustrated in the drawings described in the Examples section. The invention is capable of other embodiments or of being practiced or carried out in various ways. Also, it is to be understood that the phraseology and terminology employed herein is for the purpose of description and should not be regarded as limiting.
Soluble class I MHC-peptide complexes are invaluable reagents for characterizing immune responses involving CTLs, for measuring the affinity of MHC-TCR interactions, and for visualization of antigenspecific T cells.
One of the limitations in generating and using recombinant MHC-peptide complexes is a relatively low production efficiency. Most of the present studies utilize production methods that are based on transfection of cells with MHC constructs which express and secrete either scMHC or complexed MHC to the cell culture medium [12-23]. These complexes contain peptides from endogenously-expressed proteins which have to be subsequently exchanged with the desired peptide to be displayed in the complex [13].
Alternative methods for efficiently producing recombinant MHC-peptide complexes is by expression of the heavy chain and P-2 microglobulin separately in E. Coli. followed by co-refolding in vitro in the presence of antigenic peptide 11]. Although such methods present some advantages as compared to the eukaryotic methods described above, the quantity and purity of the MHC complex produced thereby still fall well below of that required for various studies.
While reducing the present invention to practice, it was uncovered that recombinant single chain (sc) MHC-peptide complexes produced in E. Coli constitute an efficient new way for the generation of unprecedented large amounts grams) of highly purified and functional MHC-peptide complexes. as well as MHC-peptide tetramers.
As is further described in the Examples section that follows human scMHC-peptide complexes generated from E. Coli inclusion bodies by in vitro refolding in the presence of antigenic peptides are highly pure and functional.
Thus, according to one aspect of the present invention there is provided a nucleic acid construct which includes a first nucleic acid sequence including a first polynucleotide encoding a functional human 3-2 microglobulin translationally fused upstream of a second polynucleotide encoding a functional human MHC class I heavy chain. As used herein the term "functional" when used in reference to the P-2 microglobulin and heavy chain polypeptides regions of a single chain MHC class I complex refers to any portion of each which is capable of contributing to the assembly of a functional single chain MHC class I complex capable of binding and presenting to CTLs specific antigenic peptides when complexed).
Preferably, the first polynucleotide encodes the entire P-2 microglobulin polypeptide while the second polynucleotide encodes the c 1-3 domains of the heavy chain.
The phrases "translationally fused" and "in frame" are interchangeably used herein to refer to polynucleotides which are covalently linked to form a single continuous open reading frame spanning the length of the coding sequences of the linked polynucleotides. Such polynucleotides can be covalently linked directly or preferably indirectly through a spacer or linker region.
Thus, according to a preferred embodiment of the present invention, the nucleic acid sequence further includes an in-frame linker polynucleotide. This linker polynucleotide encodes a linker peptide and is interposed between the first and said second polynucleotides.
The linker peptide is selected of an amino acid sequence which is inherently flexible, such that the polypeptides encoded by the first and said second polynucleotides independently and natively fold following expression thereof, thus facilitating the formation of a functional single chain (sc) human MHC class I complex.
According to another preferred embodiment of the present invention the linker peptide is as set forth in SEQ ID NO:
Thus, the first nucleic acid sequence of this aspect of the present invention, encodes a functional human single chain MHC class I polypeptide. Preferably, the first nucleic acid sequence is as set forth in SEQ ID NO: 4.
According to another preferred embodiment of the present invention, the first nucleic acid sequence further includes an in-frame tag sequence (such as that set forth in SEQ ID NO:20) which encodes a peptide capable of being enzymatically modified to include a binding entity. As is further described in the Examples section that follows, the binding entity, which can be, for example, biotin, can be utilized by the present invention to assemble a plurality of scMHC class I polypeptides into multimers, by providing a ligand, such as avidin or streptavidin which serves as a common attachment entity for the scMHC class I polypeptides. MHC class I multimers are particularly advantageous for peptide mediated CTL activation as is further described in the Examples section which follows.
According to another preferred embodiment of the present invention the nucleic acid construct further includes a first cis acting regulatory sequence. The cis acting regulatory sequence can include a promoter sequence and additional transcriptional or a translational enhancer sequences all of which serve for facilitating the expression of the nucleic acid sequence when introduced into a host cell. Specific examples of promoters are described hereinbelow in context of various eukaryotic and prokaryotic expression systems and in the Examples section which follows.
Thus, the nucleic acid construct of this aspect of the present invention is capable of expressing in a host cell, a recombinant human single chain MHC class I polypeptide which includes the functional human P-2 microglobulin and the functional human MHC class I heavy chain and a linker peptide interposed therebetween. Preferably, the expressible recombinant polypeptide is as set forth in SEQ ID
According to another preferred embodiment of the present invention, the nucleic acid construct also includes a second nucleic acid sequence encoding an antigenic peptide. The antigenic peptide is selected such that it is capable of binding a human MHC class I complex. As is further described in the Examples section which follows, various CTL specific antigenic peptides can be encoded by the second nucleic acid sequence, including but not limited to cancer cell derived antigenic peptides, virally derived antigenic peptides and the like.
According to another preferred embodiment of the present invention, the nucleic acid construct includes a second cis acting regulatory sequence which serves for expressing the second nucleic acid sequence when introduced into a host cell.
It will be appreciated that a single cis acting regulatory sequence can be utilized by the nucleic acid construct to direct transcription of a single transcript which includes the first and second nucleic acid sequence. In such a case, an internal ribosome entry site (IRES) can be utilized so as to allow translation of the internally positioned nucleic acid sequence.
Although co-expression of the scMHC class I polypeptide and the antigenic peptide from a single nucleic acid construct is advantageous when transforming a host cell, the two nucleic acid sequences can alternatively be included in two separate nucleic acid construct which can be utilized to co-transform a single cell.
In any case, it will be appreciated that the nucleic acid construct or constructs must be configured such that the levels of expression of both the scMHC class I polypeptide and the binding peptide thereof are stoichiometrically correct (a 1:1 peptide to scMHC class I polypeptide ratio is preferred) so as to allow efficient assembly of the scMHC class I-peptide complex.
Preferably the promoter utilized by the nucleic acid construct(s) of the present invention is a strong constitutive promoter such that high levels of expression are attained for the first and second nucleic acid sequences following host cell transformation.
It will be appreciated that high levels of expression can also be effected by transforming the host cell with a high copy number of the nucleic acid construct, or by utilizing cis acting sequences which stabilize the resultant transcript and as such decrease the degradation or "turn-over" of such a transcript.
According to another aspect of the present invention there is provided a transformed host cell including the nucleic acid construct or constructs described above.
As used herein, the phrase "transformed cell" describes a cell into which an exogenous nucleic acid sequence is introduced to thereby stabily or transiently genetically alter the host cell. It may occur under natural or artificial conditions using various methods well known in the art some of which are described in detail hereinbelow in context with specific examples of host cells.
The transformed host cell can be a eukaryotic cell, such as, for example, a mammalian cell, an insect cell, a plant cell, a yeast cell and a protozoa cell, or alternatively, the cell can be a bacterial cell.
When utilized for eukaryotic host cell expression, the nucleic acid construct(s) according to the present invention can be a shuttle vector, which can propagate both in E. coli (wherein the construct comprises an appropriate selectable marker and origin of replication) and be compatible for expression in eukaryotic host cells. The nucleic acid construct(s) according to the present invention can be, for example, a plasmid, a bacmid, a phagemid, a cosmid, a phage, a virus or an artificial chromosome.
According to another preferred embodiment of the present invention the host cell is a mammalian cell of, for example, a mammalian cell culture. Suitable mammalian expression systems include, but are not limited to, pcDNA3, pcDNA3.1(+/-), pZeoSV2(+/-), pSecTag2, pDisplay, pEF/myc/cyto, pCMV/myc/cyto, pCR3.1, which are available from Invitrogen, pCI which is available from Promega, pBK-RSV and pBK-CMV which are available from Stratagene, pTRES which is available from Clontech, and their derivatives.
Insect cell cultures can also be utilized to express the nucleic acid sequences of the present invention. Suitable insect expression systems include, but are not limited to the baculovirus expression system and its derivatives which are commercially available from numerous suppliers such as Invitrogen (maxBac Clontech (BacPak), or Gibco (Bac-to-Bac'T).
Expression of the nucleic acid sequences of the present invention can also be effected in plants cells. As used herein, the phrase "plant cell" can refer to plant protoplasts. cells of a plant tissue culture, cells of plant derived tissues or cells of whole plants.
There are various methods of introducing nucleic acid constructs into plant cells. Such methods rely on either stable integration of the nucleic acid construct or a portion thereof into the genome of the plant cell, or on transient expression of the nucleic acid construct in which case these sequences are not stabily integrated into the genome of the plant cell.
There are two principle methods of effecting stable genomic integration of exogenous nucleic acid sequences such as those included within the nucleic acid construct of the present invention into plant cell genomes:
Agrobacterium-mediated gene transfer: Klee et al. (1987) Annu. Rev. Plant Physiol. 38:467-486; Klee and Rogers in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes, eds. Schell, and Vasil, L. Academic Publishers, San Diego, Calif. (1989) p. 2-25; Gatenby, in Plant Biotechnology, eds. Kung. S. and Arntzen, C. Butterworth Publishers, Boston, Mass. (1989) p. 93-112.
(ii) direct DNA uptake: Paszkowski et al., in Cell Culture and Somatic Cell Genetics of Plants, Vol. 6, Molecular Biology of Plant Nuclear Genes eds. Schell, and Vasil, L. Academic Publishers, San Diego, Calif. (1989) p. 52-68; including methods for direct uptake of DNA into protoplasts, Toriyama, K. et al. (1988) Bio/Technology 6:1072-1074. DNA uptake induced by brief electric shock of plant cells: Zhang et al. Plant Cell Rep. (1988) 7:379-384. Fromm et al. Nature (1986) 319:791-793. DNA injection into plant cells or tissues by particle bombardment, Klein et al. Bio/Technology (1988) 6:559-563; McCabe et al. Bio/Technology (1988) 6:923-926; Sanford, Physiol. Plant. (1990) 79:206-209; by the use of micropipette systems: Neuhaus et al., Theor. Appl. Genet. (1987) 75:30-36; Neuhaus and Spangenberg, Physiol. Plant. (1990) 79:213-217; or by the direct incubation of DNA with germinating pollen, DeWet et al. in Experimental Manipulation of Ovule Tissue, eds. Chapman, G. P. and Mantell, S. H. and Daniels, W. Longman, London, (1985) p. 197-209; and Ohta. Proc. Natl. Acad. Sci. USA (1986) 83:715-719.
The Agrobacterium system includes the use of plasmid vectors that contain defined DNA segments that integrate into the plant genomic DNA. Methods of inoculation of the plant tissue vary depending upon the plant species and the Agrobacterium delivery system. A widely used approach is the leaf disc procedure, see for example, Horsch et al. in Plant Molecular Biology Manual A5, Kluwer Academic Publishers, Dordrecht (1988) p. 1-9. A supplementary approach employs the Agrobacterium delivery system in combination with vacuum infiltration. The Agrobacterium system is especially viable in the creation of stabily transformed dicotyledenous plants.
There are various methods of direct DNA transfer into plant cells. In electroporation, protoplasts are briefly exposed to a strong electric field. In microinjection, the DNA is mechanically injected directly into the cells using very small micropipettes. In microparticle bombardment, the DNA is adsorbed on microprojectiles such as magnesium sulfate crystals, tungsten particles or gold particles, and the microprojectiles are physically accelerated into cells or plant tissues. Direct DNA transfer can also be utilized to transiently transform plant cells.
In any case suitable plant promoters which can be utilized for plant cell expression of the first and second nucleic acid sequences, include, but are not limited to CaMV 35S promoter, ubiquitin promoter, and other strong promoters which can express the nucleic acid sequences in a constitutive or tissue specific manner.
Plant viruses can also be used as transformation vectors. Viruses that have been shown to be useful for the transformation of plant cell hosts include CaV, TMV and BV. Transformation of plants using plant viruses is described in U.S. Pat. No. 4,855,237 (BGV), EP-A 67,553 (TMV), Japanese Published Application No. 63-14693 (TMV), EPA 194,809 EPA 278,667 and Gluzman, Y. et al., Communications in Molecular Biology: Viral Vectors, Cold Spring Harbor Laboratory, New York, pp. 172-189 (1988). Pseudovirus particles for use in expressing foreign DNA in many hosts, including plants, is described in WO 87/06261.
Construction of plant RNA viruses for the introduction and expression of non-viral exogenous nucleic acid sequences in plants is demonstrated by the above references as well as by Dawson, W. O. et al., Virology (1989) 172:285-292; Takamatsu et al. EMBO J. (1987) 6:307-311; French et al. Science (1986) 231:1294-1297; and Takamatsu et al. FEBS Letters (1990) 269:73-76.
When the virus is a DNA virus, the constructions can be made to the virus itself. Alternatively, the virus can first be cloned into a bacterial plasmid for ease of constructing the desired viral vector with the nucleic acid sequences described above. The virus can then be excised from the plasmid. If the virus is a DNA virus, a bacterial origin of replication can be attached to the viral DNA, which is then replicated by the bacteria. Transcription and translation of this DNA will produce the coat protein which will encapsidate the viral DNA. If the virus is an RNA virus, the virus is generally cloned as a cDNA and inserted into a plasmid. The plasmid is then used to make all of the constructions. The RNA virus is then produced by transcribing the viral sequence of the plasmid and translation of the viral genes to produce the coat protein(s) which encapsidate the viral RNA.
Construction of plant RNA viruses for the introduction and expression in plants of non-viral exogenous nucleic acid sequences such as those included in the construct of the present invention is demonstrated by the above references as well as in U.S. Pat. No. 5,316,931.
Yeast cells can also be utilized as host cells by the present invention. Numerous examples of yeast expression vectors suitable for expression of the nucleic acid sequences of the present invention in yeast are known in the art. Such vectors are usually introduced in a yeast host cell via chemical or electroporation transfornation methods well known in the art. Commercially available systems include, for example, the pYESTM (Invitrogen) or the YEXTn (Clontech) expression systems.
It will be appreciated that when expressed in eukaryotic expression systems such as those described above, the nucleic acid construct preferably includes a signal peptide encoding sequence such that the polypeptides produced from the first and second nucleic acid sequences are directed via the attached signal peptide into secretion pathways. For example, in mammalian, insect and yeast host cells, the expressed polypeptides can be secreted to the growth medium, while in plant expression systems the polypeptides can be secreted into the apoplast, or directed into a subcellular organelle.
According to a presently preferred embodiment of the present invention, the host cell is a bacterial cell, such as for example, E. coli. A bacterial host can be transformed with the nucleic acid sequence via transformation methods well known in the art, including for example, chemical transformation CaCl 2 or electroporation.
Numerous examples of bacterial expression systems which can be utilized to express the nucleic acid sequences of the present invention are known in the art. Commercially available bacterial expression systems include, but are not limited to, the pETTN' expression system (Novagen), pSE T expression system (Invitrogen) or the pGEXT" expression system (Amersham).
As is further described in the Examples section which follows, bacterial expression is particularly advantageous since the expressed polypeptides form substantially pure inclusion bodies readily amenable to recovery and purification of the expressed polypeptide.
Thus, according to yet another aspect of the present invention there is provided a preparation of bacterial derived inclusion bodies which are composed of over 30 percent, preferably over 50 o, more preferably over 75 most preferably over 90 by weight of the recombinant human scMHC class I polypeptide of the present invention. The isolation of such inclusion bodies and the purification of the scMHC class I polypeptide therefrom are described in detail in the Examples section which follows.
As demonstrated in the Examples section, bacterial expression of an scMHC class I polypeptide can provide high quantities of pure and functional scMHC class I polypeptides. As such, the method of the present invention can also be applied to bacterially express mammalian scMHC class I polypeptides which to date have been expressed, with limited success, only in eukaryotic expression system.
Thus, according to another aspect of the present invention there is provided a nucleic acid construct which includes a nucleic acid sequence including a first polynucleotide encoding a functional mammalian P-2 microglobulin. The nucleic acid construct further includes a second polynucleotide encoding a functional mammalian MHC class I heavy chain which is translationally fused downstream of the first polynucleotide. The nucleic acid construct according to this aspect of the present invention further includes a cis acting regulatory sequence which is capable of directing expression of the nucleic acid sequence in bacteria. Suitable promoters and bacterial expression systems which can be utilized to express the nucleic acid sequence of this aspect of the present invention are described hereinabove.
The nucleic acid sequence according to this aspect of the present invention can be derived from, or synthesized according to, the MHC class I encoding sequences of a mammal such as, but not limited to, a mouse, a rat, a pig, and a rabbit.
It will be appreciated that the various embodiments described hereinabove for the human scMHC class I construct, which embodiments apply and are useful for the bacterial expression of the mammalian scMHC class I nucleic acid construct of this aspect of the present invention are also incorporated herein.
Thus, the present invention provides various expression constructs which can be utilized to express scMHC class I polypeptides preferably along with binding peptides thereof in eukaryotic or preferably bacterial expression systems.
According to an additional aspect of the present invention there is provided a method of producing a functional MHC class I molecule, the method according to this aspect of the present invention utilizes any of the nucleic acid constructs described for expressing, in bacteria, a single chain MHC class I polypeptide including a functional mammalian P-2 microglobulin amino acid sequence directly or indirectly covalently linked to a functional mammalian MHC class I heavy chain amino acid sequence.
Following expression, the single chain MHC class I polypeptide is isolated and purified as described below.
As is further described in the Examples section which follows, the expressed polypeptide forms substantially pure inclusion bodies which are readily isolated via fractionation techniques well known in the art and purified via for example denaturing-renaturing steps.
Preferably, the single chain MHC class I polypeptide is renatured and refolded in the presence of an antigenic peptide capable of binding the single chain MHC class I polypeptide. As is further described in the Examples section this enables to generate a substantially pure MHC class I-antigenic peptide complex which can further be purified via size exclusion chromatography.
It will be appreciated that the antigenic peptide used for refolding can be co-expressed along with the single chain MHC class I polypeptide in the bacteria. In such a case the expressed polypeptide and peptide coform inclusion bodies which can be isolated and utilized for MHC class I-antigenic peptide complex formation.
Additional objects, advantages, and novel features of the present invention will become apparent to one ordinarily skilled in the art upon examination of the following examples, which are not intended to be limiting. Additionally, each of the various embodiments and aspects of the present invention as delineated hereinabove and as claimed in the claims section below finds experimental support in the following examples.
EXAMPLE S
Reference is now made to the following examples, which together with the above descriptions, illustrate the invention in a non limiting fashion.
Generally, the nomenclature used herein and the laboratory procedures utilized in the present invention include molecular, biochemical, microbiological and recombinant DNA techniques. Such techniques are thoroughly explained in the literature. See, for example, "Molecular Cloning: A laboratory Manual" Sambrook et al., (1989); "Current Protocols in Molecular Biology" Volumes I-III Ausubel, R. M., ed. (1994); Ausubel et al., "Current Protocols in Molecular Biology", John Wiley and Sons, Baltimore, Maryland (1989); Perbal, "A Practical Guide to Molecular Cloning", John Wiley Sons, New York (1988); Watson et al., "Recombinant DNA", Scientific American Books. New York; Birren et al. (eds) "Genome Analysis: A Laboratory Manual Series", Vols. 1-4, Cold Spring Harbor Laboratory Press, New York (1998); methodologies as set forth in U.S. Pat. Nos. 4,666,828; 4,683,202; 4,801,531; 5,192,659 and 5,272,057; "Cell Biology: A Laboratory Handbook", Volumes I-III Cellis, J. ed. (1994); "Current Protocols in Immunology" Volumes I-III Coligan J. ed. (1994): Stites et al. (eds), "Basic and Clinical Immunology" (8th Edition), Appleton Lange, Norwalk, CT (1994); Mishell and Shiigi (eds), "Selected Methods in Cellular Immunology", W. H. Freeman and Co., New York (1980); available immunoassays are extensively described in the patent and scientific literature, see, for example, U.S. Pat. Nos. 3,791,932; 3,839,153; 3,850,752; 3,850,578; 3,853,987; 3,867,517; 3,879,262; 3,901,654; 3,935,074; 3,984,533; 3,996,345; 4,034,074; 4,098,876; 4,879,219; 5,011,771 and 5,281,521; "Oligonucleotide Synthesis" Gait, M. ed. (1984); "Nucleic Acid Hybridization" Hames, B. and Higgins S. eds. (1985); "Transcription and Translation" Hames, B. D., and Higgins S. eds. (1984); "Animal Cell Culture" Freshney, R. ed. (1986); "Immobilized Cells and Enzymes" IRL Press, (1986); "A Practical Guide to Molecular Cloning" Perbal, (1984) and "Methods in Enzymology" Vol. 1-317, Academic Press; "PCR Protocols: A Guide To Methods And Applications", Academic Press, San Diego, CA (1990); Marshak et al., "Strategies for Protein Purification and Characterization A Laboratory Course Manual" CSHL Press (1996); all of which are incorporated by reference as if fully set forth herein. Other general references are provided throughout this document. The procedures therein are believed to be well known in the art and are provided for the convenience of the reader. All the information contained therein is incorporated herein by reference.
EXAMPLE 1
Materials and methods
Peptides:
Cancer cell associated peptides G9-209-2M (IMDQVPFSV SEQ ID NO:1) and G9-280-9V (YLEPGPVTV SEQ ID NO:2), both derived from the melanoma antigen gpl00 [24-26] were used for MHC binding. These peptides are modified at the MHC anchor positions 2 (in G9-209-2M) and 9 (in G9-280-9V) to improve the binding affinity to HLA-A2 The TAX 11-19 HTLV-1 peptide (LLFGYPVYV, SEQ ID NO:3) was used as a control [53].
Peptides were synthesized by standard fluorenylmethoxycarbonyl chemistry and purified to >95% by reverse phase HPLC. Following purification these peptides were used in the refolding of the single-chain MHC (scMHC)-peptide complexes and formation of scMHC-peptide tetramers further described hereinunder.
Plasmid constructs:
A cDNA construct (SEQ ID NO:4) encoding a single-chain (sc) MHC polypeptide (SEQ ID NO:5) which includes the first three extracellular domains of HLA-A2 (HLA-A*0201, amino acids 1-275 SEQ ID NO:6) and the human p2-microglobulin polypeptide (SEQ ID NO:7) was assembled from cl-3 and P-2 microglobulin cDNAs derived from the human T-B cell hybrid T2 cell line The human P-2 microglobulin was PCR amplified using b2M-5, AGGAGATATACATATGATCCAGCGTACTCCAAAGAT-3' (SEQ ID NO:8) and b2M-3,
GAATTCATTACA TGTCTCGATrccCACTTAAC-3' (SEQ ID NO:9) and linked via a 15 residue flexible linker (Glv-Ser4) 3 (SEQ ID
to the N-terminus of the HLA-A2 heavy chain which wvas PCR amplified using A2-5,
CATGAGGTA-3)' (SEQ ID NO: 11) and A2-3. CGGGCTh[GTTAGCAGCCGAATTFCATTAGGT GAGGGGCTTG GGCAA-' (SEQ ID NO: 12) primers. The cDNAs of both chains were used in a two-step PCR overlap extension reaction in which the of the P3-2 microglobulin was connected to the 5'-end of the HLA-A2 gene.
To perform the two-step PCR overlap extension reaction. two thirds of the linker sequence were introduced into the P3-2 microglobulin sequence using PCR primers b2M-L5.
TTGGCGCATATGATCCAGCGTACTCCAAAGATT-3' (SEQ ID NO:13) and b2M-L3,
CTGGTGGAGGTGGCAGCGGCTCTCACTCCATGA--3' (SEQ ID NO:14) while PCR primers
CTCTGGTGGAGGTGGCAGCGGCTCTCACTCCATGA-3' (SEQ ID NO: 15) and A2-L3, 5'-G GGAG AATTCTTACTCCCATCTCA GGGTGAGGGGCTTGGGCAA-3' (SEQ ID NO: 16) were utilized for HLA-A2.
Following amplification, the P3-2 microglobulin and HLA-A2 PCR products were combined in a 1: 1 ratio and the reaction was PCR amplified using the b2M-L5 and A2-L3 primers. the PCR product was purified and subcloned into the pET-based expression vector.
The HLA-A2 and P-2 microglobulin coding sequences described above were also separately cloned in pET2 1 for independent expression. The scMIHC-BirA coding sequence included a peptide sequence for site specific biotinylation (LGGIFEAMKM\IELRD, SEQ ID NO:17), the lysine residue biotinylated underlined) linked to HLA-A2 N-terminus via a short linker (QSTRGGASGGG, SEQ ID NO: 18).
The scMHC-BirA coding sequence was PCR amplified using B2M-BirA-3, CAGTAAAAGCTTTTTATCAGCCTCCGAACTGTG GATGCCTCCACGCCGAACCTCCACCAGAACCACCTCCGGACCC GCCACCTCCCTCCCATCTCAGGGT (SEQ ID NO:19) and the primer described above. The resultant PCR product which included the BirA recognition sequence (SEQ ID NO:20) was purified digested and subcloned into a bacterial expression vector as shown in Figure 1.
Expression, refolding and purification of scMHC-peptide complexes:
The HLA-A2, P-2 microglobulin, and the scMHC constructs subcloned into pET21 were expressed under IPTG induction, forming intracellular inclusion bodies in BL-21 DE3 cells. Inclusion bodies were isolated and purified from the induced BL21 cells and solubilized in 6M Guanidine HCI pH 7.4. Following reduction with mM DTE, inclusion bodies were refolded in a redox-shuffling buffer system (0.1M Tris, 0.5M Arginine, 0.09 mM oxidized glutathione, pH 8.0) in the presence of a 5 to 10 molar excess of the antigenic peptides derived from gpl00 or the HTLV-1 viral peptide described above [26,52]. Following refolding, the protein was dialyzed and concentrated by a Minisette system (Filtron, Northborough, MA) using a 10K cutoff cassette. Soluble scMHC-peptide complexes were purified by sizeexclusion chromatography on TSK3000 column (TOSOHAAS, Montgomeryville, PA) using PBS as an elution buffer.
Biotinylation of recombinant scMHC-peptide complexes and tetrameric scMHC-peptide complexes:
The s.c-b2-A2-BirA (scMHC-BirA) construct also expressed as described above was refolded in the presence of peptides 209 or 280 and purified by size exclusion chromatography on TSK3000 column (elution buffer 10mM Tris-HCI pH The scMHC-peptide-BirA complexes were concentrated to 20-30 mM (1-1.5 mg/ml) by Centricon 30 (Amicon) and were then subjected to enzymatic biotinylation for 16 hr at 25 °C using a biotin protein ligase- Bir A enzyme (AVIDITY). Buffer exchange and removal of excess biotin from the biotinylated complexes was performed using Centricon 30 ultrafiltration. Tetrameric arrays of biotinylated scMHC-peptide complexes were formed by the addition of streptavidin (Sigma, St. Louis, MO) at a molar ration of 4:1 scMHC-peptide complex:streptavidin, respectively.
ELISA assays:
Maxisorb immunoplates (Nalge Nunc, Naperville, IL) were coated overnight at 4 oC with 1 mg/ml anti-HLA monoclonal antibody BB7.2 (ATCC HB82) and blocked using PBS including 3% BSA. Biotinylated anti-HLA W6/32 monoclonal antibody (ATCC HB95) was used for detection of the HLA complex via a streptavidin-peroxidase conjugate. In addition, biotinylated or unbiotinylated scMHC-BirA-peptide complexes were incubated for 30 minutes at room temperature with washed MagnaBind T M streptavidin beads (PIERCE). An external magnetic field was used to separate biotinylated complexes bound to the beads from unbound material. The supernatant was pooled away from bound sample and the beads were blocked with 2% MPBS (PBS 2% semi-skimmed milk powder) for 30 minutes at room temperature. The biotinylated complexes were subsequently assayed with the conformational dependent antibody W6/32 and detected using antimouse IgG-peroxidase.
Circular dichroism spectra:
The circular dichroism spectra of the scMHC-peptide complexes was measured at room temperature via a spectropolarimeter (JASCO 500) adjusted to a sensitivity of 0.5 mdeg/cm and a scan speed of nm/minute. Scans were performed between 195 and 275 nm at a protein concentration of 0.27 mg/ml. Secondary structure calculations were measured using published programs [29].
Mass spectrometry analysis of scMHC-peptide complexes:
The scMHC-peptide complex was resolved by HPLC on a 2.1x30 mm C-8 column (AQUAPORE RP-300, Applied Biosystems), and eluted with a linear gradient of 15-65% Acetonitrile (ACN) in 0.05 TFA, at 1.25 %/min and a flow rate of 150 ml/min. The sample was microsprayed directly from the HPLC column into an electrospray (ESI) ion trap mass spectrometer (LCQ, Finnigan) and analyzed in the positive ion mode. The mass estimation of the proteins was done using the deconvolution algorithm, which transforms an ESI mass spectral plot of relative abundance versus mass-to-charge ratios into a plot of relative abundance versus mass. Each sequence of multiply charged ion peaks in the acquired mass spectrum corresponding to one sample component, is converted into a single peak positioned at the molecular mass M in the deconvoluted spectrum.
CTL clones and CTL stimulation assays:
CTL clones specific for melanoma peptides were provided by Drs. Steven Rosenberg and Mark Dudley (Surgery Branch, National Cancer Institute, N1H). These CTL clones were generated by cloning from bulk cultures of PBMCs acquired from patients receiving peptide immunizations The CTLs were subsequently expanded using irradiated PBMCs and the OKT3 antibody (30 ng/ml) in the presence of 50 CU/ml IL-2.
The CTL clones (1xl0 5/well) were washed in tissue culture medium and incubated in duplicates or triplicates with various concentrations of scMHC-peptide tetramers in tissue culture medium for 24 hrs at 37 OC. Alternatively scMHC-peptide complexes were immobilized onto Maxisorb immunoplates over night at 4 OC and CTL clones in culture medium were subsequently added to the wells and incubated for 24 hrs at 37 Following incubation supernatants were collected via centrifugation and IL-2 and IFNg present in the culture supernatants were assayed using a double sandwich antibody-capture enzyme immunoassay (Flexia, BIOSOURCE, Camarillo. CA). Concentrations of IL-2 and INFy in the culture supernatants was determined against a calibration curve of recombinant human IL-2 and INFy.
Experimental Results
Expression, refolding, and purification of recombinant singlechain MHC-peptide complexes:
To produce soluble single-chain MHC-peptide complexes we subcloned the genes encoding the single-chain MHC into a pET system expression vector in which expression is driven by the phage T7 promoter.
The plasmid constructs used in this study are described in Figure 1. Expression of the scMHC gene in E. Coli BL21 cells was very efficient and recombinant protein accumulated as insoluble intracellular inclusion bodies. The single-chain MHC could be detected as the major band on SDS-PAGE of solubilized whole cells (not shown) as well as isolated purified inclusion bodies (Figure 2a). The expression of the scMHC protein was comparable to that of the separate components, i.e., the transmembrane HLA-A2 heavy chain and P-2 microglobulin (Figure 2a). Very efficient expression was also obtained for the scMHC-BirA protein (Figure 2b). Purified inclusion bodies contained 80-90% recombinant scMHC. Inclusion bodies were purified, solubilized in 6M guanidine HCI, and refolded by in vitro redox-shuffling buffer system in the presence of the antigenic peptide. Three different peptides were used for the refolding of the scMHC molecules. Two peptides (G9-209-2M and G9-280-2V) are tumor associated antigens derived from the melanoma common antigen gpl00 [26] and one is a viral peptide derived from HTLV-1 (TAX) These peptides were previously shown to be HLA-A2 restricted [26, 53].
Refolded complexes were dialyzed and concentrated following by purification using size-exclusion chromatography on TSK3000 column. SDS-PAGE analysis, under non-reducing conditions, of refolded purified scMHC-peptide complexes revealed a homogenous monomeric population of molecules that migrated as a uniform single band corresponding to the scMHC molecule with an apparent molecular weight of 45 kDa as calculated according to the relative migration against a set of molecular weight markers (Figures 3a-b). As shown in Figures 3a-b, purified homogenous scMHC-peptide complexes were obtained with all three peptides tested. When the scMHC was refolded in the absence of a peptide, a highly aggregated protein was observed which was composed of a heterogeneous population of molecules as analyzed by size-exclusion chromatography and SDS-PAGE; this indicates the existence of an unstable population of molecules that are improperly folded (data not shown). Thus, a uniform population of monomers could only be found in the peptide-induced refolding preparations. The refolded scMHC-peptide complexes could be stored at 0C and re-used upon thawing. The yield of the refolded purified single-chain MHC complexes was 20-25%, i.e. 20-25 mg of a purified soluble complex was obtained from 100 mg of refolded inclusion body protein.
Similar results were obtained with the scMHC-BirA-peptide complexes, which contain a sequence tag at the C-terminus of the HLA-A2 for site specific biotinylation (Figure 3c). The apparent molecular weight of 48 kDa was observed for this molecule reflecting the increase in the size of the scMHC molecule predicted by the addition of the BirA peptide tag and the linker connecting it to HLA-A2.
Thus, these results indicate that scMHC-peptide complexes can be produced in vitro by refolding of E. Coli inclusion bodies in the presence of antigenic peptides. The refolding process is efficient and a homogenous population of complexes can be obtained with high yields and purity.
Biochemical and biophysical characterization of single-chain MHC-peptide complexes:
As shown in Figures 3a-c, the scMHC peptide complexes were pure and homogenous as judged by SDS-PAGE. Size exclusion chromatography on TSK3000 column revealed that the purified scMHC-peptide complexes were eluted as monomers with a molecular mass of kDa (not shown). When purified complex was concentrated by ultrafiltration to 0.15 mM mg/ml) and analyzed for its molecular form on a size-exclusion TSK3000 column in PBS, no indication of dimerization or aggregation, was observed.
To evaluate the secondary structure of the single-chain MHC-peptide complex, protein purified through size-exclusion chromatography was examined by circular dichroism (CD) spectroscopy (Figure 4). Spectra of the complex showed a characteristic minima at 218 nm consistent with a largely p-sheet structure. When analyzed for secondary structural calculations the spectra was indicative of a specific pattern of secondary structure which included 62% P-sheet, 5% p-turn, 8% a-helix and 18% aromatic side chain contribution. When denatured at 800C, the spectra profile indicated that most of the characteristic p-sheet structure was lost and the CD signal increased, indicating random coils were generated as the result of denaturation. The melting curve showed that the complex containing peptide G9-209-2M was thermally stable with a melting temperature of approximately 60 oC (data not shown). These results are similar to the thermal denaturation curves for HLA-A2 complexed with influenza virus peptides as was monitored by the change in the CD signal at 218 nm Thus it is concluded that these secondary structure features are characteristic of correct folded MHC-peptide complexes [31].
The refolded scMHC-peptide complexes were also assayed using conformation-specific antibodies, which recognize MHC-peptide complexes only when correctly folded. As shown in Figure 5a, the anti-HLA-A2 specific mAb fragment BB7.2 and the mAb fragment W6/32 specific for a fully assembled, peptide-containing HLA-A2 detected soluble scMHC-peptide complex. The W6/32 antibody specifically recognized, in a concentration dependent manner, the refolded and purified scMHC complexes containing any of the two gpl00-derived peptides indicating that the complexes are correctly folded, include the peptide, and are structurally functional.
The specific recognition of the scMHC-BirA-pcptide complex by the W6/32 antibody was also effected when tested on biotinylated complexes that were immobilized to streptavidin-coated magnetic beads (Figure 5b). The ELISA signal from beads coated with biotinylated scMHC-BirA-peptide complexes was 5-6 fold higher than scMHC-BirA-peptide complex that was not subjected to biotinylation by the BirA enzyme (Figure
Mass spectrometry analysis was performed in order to clearly demonstrate that the scMHC complex is folded correctly and includes the peptide. The refolded and purified complex was concentrated to 6 mg/ml and a sample was subjected to analysis by electron spray mass spectrometry. The complex sample was first resolved by HPLC on a C-8 column and was subsequently eluted with a linear gradient of Acetonitrile in TFA, leading to separate sequential clution of the peptide and the scMHC protein from the column. The eluted samples were microsprayed directly from the HPLC column into an electrospray ion trap (ESI) mass spectrometer and analyzed in the positive ion mode. As shown in Figure 6a, the eluted peptide has an expected mass of 1035 dalton which corresponds to the mass of the G9-209-2M peptide used for refolding the scMHC-peptide complex. This was the only peptide detected indicating that the refolded complex is a homogenous population of molecules containing a single specific peptide. A minor peak with a mass of 1057 daltons was also observed, which peak corresponds to the G9-209-2M peptide including a sodium ion which probably originated from the PBS buffer in which the complex was purified and concentrated prior to analysis.
The mass estimation of the scMHC complex was done using a deconvolution algorithm, which transforms an ESI mass spectral plot of relative abundance versus mass-to-charge ratios into a plot of relative abundance versus mass (Figures 6b-c). Each sequence of multiply charged ion peaks in the acquired mass spectrum (Figure 6b) which corresponds to one sample component, is converted into a single peak positioned at the molecular mass in the deconvoluted spectrum (Figure 6c). As shown in Figure 6c, the deconvoluted spectrum revealed a single peak with a mass of 44.6 kDa corresponding to the expected molecular weight of the scMHC protein. As shown above for the peptide, this was the only identified protein peak in the analyzed spectrum indicating that the protein consists of a very homogenous population of folded complexes. Stoichiometric estimation of the eluted peptide performed according to the mass spectrometry data, revealed that all refolded and purified scMHC molecules are complexed with the peptide.
Biological activity of single-chain MHC-peptide complex:
A CTL stimulation assay using cloned CTLs specific for the G9-209-2M peptide was utilized to test the biological activity of the scMHC-peptide complex.
The recombinant purified scMHC complexes that were produced with the 3 different peptides (G9-209-2M, G9-280-9V, and TAX) were immobilized on a microtiter plate and tested for the ability to induce CTL activation. As shown in Figure 7a, the single-chain MHC molecule bound to the g9-209-2M peptide induced specific activation of the g209-specific CTL clone R6C12 as determined by interferon-g levels. On the other hand, the complexes bound to the g280 and TAX peptides did not induce specific activation.
The ability of soluble scMHC-peptide complexes to induce T cell activation in solution was also tested by generating scMHC-peptide tetramers composed of the scMHC-peptide molecule containing the sequence tag for site-specific biotinylation. Refolded and purified peptide containing scMHC-BirA complexes were biotinylated using the BirA enzyme. Following purification of the scMHC-BirA complexes tetramers were formed by incubation with streptavidin. The biological activity of the scMHC-BirA-peptide tetramers was tested by their ability to activate the corresponding CTL clone. As shown in Figure 7b, the tetramers containing the G9-209-2M peptide activated the 209-specific CTL clone R6C12 but not the Mart-I specific CTL clone JB2F4, indicating that the refolded scMHC-peptide complexes are functional and specific. As shown in Figure 7c, the ability of the single-chain MHC-BirA-peptide complex to activate the 209-specific CTL clone was tested with or without biotinylation of the scMHC protein and subsequent incubation with streptavidin. As is evident from this Figure, only the biotinylated MHC-BirA-peptide which can form tetrameric complexes activates the 209-specific CTL clone in solution thus T cell activation in solution requires the multimerization of MHC complexes in the form of tetramers which increase the avidity of MHC-TCR interactions.
The tetramer-induced activation of the specific G9-209-2M CTL clone was more efficient compared to the activation induced by high density coating of microtiter plates since tetramers were still able to induce specific CTL activation at ng/ml concentrations (Figure 7a and 7c).
Microscopic observation of the activated CTL clone incubated with the 209-containing MHC tetramers revealed that the cells were agglutinated (Figure 8b). This is due to the significant increase in TCR-MHC avidity and enhanced cell to cell contacts effected by multipoint binding and cross-linking which is caused by the high concentration of MHC tetramers. These morphological effects were peptide specific and dependent on the concentration of the scMHC-BirA-peptide tetramer (Figure 8b). As the concentration of tetramers increased, increased cell agglutination (rosette formation) was observed (Figure 8c-e).
CTLs incubated with unbiotinylated scMHC-BirA-209 complexes and streptavidin which served as a control did not exhibit any morphological appearance indicating of cell agglutination (Figure 8a). In addition, no agglutinations were observed when the Mart-I specific CTL clone was incubated with the same concentrations of the g209-specific scMHC tetramer (data not shown).
The scMHC tetramers also specifically stained CTL clones which recognize the MART-1 specific peptide 27-35. As shown in Figure 9a, the scMHC/Mart tetramers specifically recognized a specific CTL clone which was not recognized by the scMHC/TAX tetramers (Figure 9b). ScMHC tetramers were also used to stain a heterogeneous population of T cells from the spleen of transgenic mice (expressing HLA-A2) which were immunized with the TAX peptide. As shown in Figures O1a-b the number of specific high avidity CD8+ T cells increased by 2-fold when non-immunized and immunized mice were compared. Thus, as clearly demonstrated above, the scMHC tetramers generated according to the teachings of the present invention can be utilized to detect and isolate antigen-specific subpopulations of T cells.
The results presented herein suggest that the recombinant scMHC-peptide complexes generated according to the teachings of the present invention are functional, in both the monomeric and the complexcd tetrameric forms.
Recombinant MHC-peptide tetramers are a very important tool for the molecular characterization of immune responses.
Fluorogenic tetramers have been widely used for the characterization of CTL responses and have afforded many advantages over previous techniques, particularly the ability to directly quantify and phenotype Ag-specific CTLs ex-vivo [32-35,38-53].
It has also been suggested that the enhanced avidity afforded by multimerization of peptide-MHC may allow binding to TCRs with affinities too low to ever generate ligand-induced physiological responses. MHC-peptide tetramers can be utilized in the field of viral infections for direct visualization of antigen-specific CD8+ cells in HIV and EBV infection [33,40,41,45,52], animal models of viral infection [41-43] and direct analysis of viral-specific CD8+ cells in patients with human T cell lymphotropic virus-associated myelopathy [53].
MHC-peptide tetramers might also revolutionize the field of anti cancer immunity and immunotherapy since they can be used to identify low frequency anti-tumor CD8+ immune responses. This has already been demonstrated for melanoma where there is now considerable evidence that human tumors often express antigens that make them susceptible to lysis by CTLs [32,36,37,48,50]. Recently the use of tetrameric soluble class I MHC-peptide complexes for characterizing melanoma-specific CTL ex vivo has been reported An important advance in the field of immunotherapy will be the ability to use this technology for adoptive immunotherapy. The use of MHC-peptide tetramers will allow the isolation of CTLs specific for one melanoma epitope from a mixed CTL population using tetramer-driven cell sorting Polyclonal CTL lines can be utilized to efficiently lyse autologous tumor cells by generating monoclonal CTL lines against different melanoma epitopes using direct tetramer-driven CTL cloning [24,36]. The ability to efficiently generate recombinant MHC-peptide complexes, with a large variety of antigenic peptides, all related to one disease (for example, melanoma) may lead to improved modes of immunotherapy. Thus, it will enable the simultaneous isolation and cloning of tumor-specific CTL clones specific for the various peptides ex-vivo, accompanied with specific PCR typing of tumor associated peptides expressed by individual patients. The specific clones an then be expanded for adoptive transfer according to the tumor associated antigen (peptide) expression profile of the patient.
The availability of recombinant MHC-peptide complexes is of interest not only for the generation of MHC tetramers but also for rapid, sensitive, and reliable MHC-peptide binding assay to identify high affinity MHC binding T cell epitopes. These complexes can be used also in in-vitro primary CTL induction studies to define, among the various MHC binders, those peptides that are immunogenic.
They may be used for CTL in vitro induction as well as for the generation of MHC-peptide tetramers and analysis of specific immune responses or direct selection of CTLs from heterogeneous lymphocyte populations by tetramer-driven cell sorting and analysis.
Recombinant scM-IC-peptide complexes can also be used to generate specific antibodies by phage display technology Such antibodies with TCR-like specificity can be a valuable tool for studying antigen presentation by tumor cells as well as to develop novel targeting agents for immunotherapy.
To date, studies utilizing MHC complexes, some of which are described above, are limited by MHC purification methods which are incapable of producing the required amounts of soluble and functional monomeric or multimeric MHC-peptide complexes.
Thus, the expression method of the present invention provides an invaluable tool for advancing studies with MHC complexes by providing an easy and rapid method for producing large amounts of highly pure and functional monomeric or multimeric scMHC-peptide complexes without the need for arduous and time consuming purification steps such as peptide exchange employed by prior art methods. In addition, the present invention provides a novel human single chain MHC class I polypeptide which is functional in activating CTL clones when utilized as either a monomer or a multimer.
EXAMPLE 2
Co-expression of the scMHC complex and peptide It will be appreciated that the various scMHC constructs described above and any mammalian scMHC class polypeptides can also be coexpressed in E. coli or any other prokaryotic or eukaryotic expression systems along with the respective binding peptides thereof, thus negating the need for separately providing the binding peptide. Thus according to this method of the present invention, a single construct can be configured so as to express both the scMHC class I polypeptide and respective binding peptide coding sequences in the same cell by utilizing for example two separate promoters or an internal ribosome entry site. Alternatively the cells of the prokaryotic or eukaryotic expression system be co-transformed with two expression constructs each expressing a single coding sequence and each having a specific selection marker.
Suitable eukaryotic expression systems include, but are not limited to mammalian or insect cell cultures, plant protoplasts, plant cell cultures, whole plants, yeast cells or protozoan cells. It w\ill be appreciated that when expressed in eukaryotic expression systems the produced scMHC class I polypeptide preferably includes a transit peptide (signal sequence) such that the expressed polypeptide is secreted outside the cell (into the apoplast in plant cell cultures or whole plants) so as to avoid interaction with endogenous peptides.
It will be appreciated that the construct or constructs must be configured such that the levels of expression of both the scMHC class I polypcptide and the binding peptide thereof are stoichiometrically correct.
Thus, a cell which expresses both coding sequences can produce a fully functional scMHC-peptide complex which can be purified via suitable isolation protocols well known in the art. It will be appreciated that a bacterial expression system is especially advantageous in this case since the scMHC-peptide complex expressed thereby can form substantially pure inclusion bodies when suitable expression conditions are employed. Thus, even if the scMHC-peptide complex disassembles under denaturing conditions utilized for solubilizing the inclusion bodies, suitable renaturation conditions can be used so as to reform a functional scMHC-peptide complex.
Although the invention has been described in conjunction with specific embodiments thereof, it is evident that many alternatives, modifications and variations will be apparent to those skilled in the art. Accordingly, it is intended to embrace all such alternatives, modifications and variations that fall within the spirit and broad scope of the appended claims. All publications cited herein are incorporated by reference in their entirety. Citation or identification of any reference in this application shall not be construed as an admission that such reference is available as prior art to the present invention.
The foregoing embodiments are illustrative only of the principles of the invention, and various modifications and changes will readily occur to those skilled in the art. The invention is capable of being practiced and carried out in various ways and in other embodiments. It is also to be understood that the terminology employed herein is for the purpose of description and should not be regarded as limiting.
The term "comprise" and variants of the term such as "comprises" or "comprising" are used herein to denote the inclusion of a stated integer or stated integers but not to exclude any other integer or any other integers, unless in the context or usage an exclusive interpretation of the term is required.
Any reference to publications cited in this specification is not an S: 15 admission that the disclosures constitute common general knowledge in S. Australia.
e
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EDITORIAL NOTE:
listingj ragjes numbered 75 of 8 1 to 5 of b1.
SEQUENCE LISTING
<110> Reiter, Yoramn
<120> SINGLE CHAIN
SAM4E AND METHODS CLASS
OF GENERAT IIIMnG-AJrR HISTO-CONPATIBILITY
SAIA.E COMPLEXES, CC-:ZSTRUCTS ENCODING <130> 01/21870
<150> US 09/534,966
<151> 2000-03-27
<160>
<170> Patentln version
<210> 1
<211> 9
<212> PRT
<213> Artificial
<220>
<223> synthetic peptide
<400> 1
Ile Met
1 Asp Gin Val Pro Phe Ser Val
<210> 2
<211> 9
<212> PRT
<213> Artificial
<220>
<223> synthetic peptide
<400> 2
Tyr Leu
1 Glu Pro Gly Pro Val Thr Val
<210> 3
<211> 9
<212> PRT
<213> Artificial
<220>
<223> synthetic peptide
<400> 3
Leu Leu Phe Gly
1                            Tyr Pro Val Tyr Val
<210> 4
<211> 1048
<212> DNA
<213>  Homo sapiens  WO 01/72768 PCT/I LOI /00260
<400> 4
atgatccagc gtactccaaa gattcaggtt tactcacgtc atccagcaga gaatggaaag
tcaaatttcc tgaattgcta tgtgtctggg tttcatccat ccgacattga agttgactta 120 ctgaagaatg gagagagaat tgaaaaagtg gagcattcag acttgtcttt cagcaaggac 180 tggtctttct atctcttgta ttatactgag ttcaccccca ctgaaaaaga tgagtatgcc 240 tgccgtgtga accacgtgac tttgtcacag cccaagatag ttaagtggga tcgagacatg 300 ggtggcggtg gaagcggcgg tggaggctct ggtggaggtg gcagcggctc tcactccatg 360 aggtatttct tcacatccgt gtcccggccc ggccgcgggg agccccgctt catcgcagtg 420 ggctacgtgg acgacacgca gttcgtgcgg ttcgacagcg acgccgcgag ccagaggatg 480 gagccgcggg cgccgtggat agagcaggag ggtccggagt attgggacgg ggagacacgg 540 aaagtgaagg cccactcaca gactcaccga gtggacctgg ggaccctgcg cggctactac 600 aaccagagcg aggccggttc tcacaccgtc cagaggatgt atggctgcga cgtggggtcg 660 gactggcgct tcctccgccg gtaccaccag tacgcctacg acggcaagga ttacatcgcc 720 ctgaaagagg acctgcgctc ttggaccgcg gcggacatgg cagctcagac caccaagcac 780 aagtgggagg cggcccatgt ggcggagcag ttgagagcct acctggaggg cacgtgcgtq 840 gagtggctcc gcagatacct ggagaacggg aaggagacgc tgcagcgcac ggacgccccc 900 aaaacgcaca tgactcacca cgctgtctct gaccatgaag ccaccctgag gtgctgggcc 960 ctgagcttct accctgcgga gatcacactg acctggcagc qgacttggag gaatctttga 1020 ggcaatgaag atggagctgc gggactga 1048
<210>
<211> 415
<212> PRT
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<223> human beta2 microglobulin linked to MHC class I heavy chain
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M1et Ile Gin Arg Thr Pro Lys Ile Gin Val Tyr Ser Arg His Pro Ala
Glu Asn Gly Lys Ser Asn Phe Leu Asn Cys
25 Tyr Val Ser Gly Phe His
Pro Ser Asp Ile Glu Val Asp Leu Leu Lys Asn Gly Glu Arg
40 Ile Glu
Lys Val Glu His Ser Asp Leu Ser Phe Ser Lys Asp Trp Ser Phe Tyr
55
Leu Leu Tyr Tyr Thr Glu Phe Thr Pro Thr Glu Lys Asp Glu
70 Tyr Ala
75
Cys Arg Val Asn His Val Thr Leu Ser Gln Pro Lys Ile Val Lys Trp
Asp Arg Asp Met Gly
100 Gly Gly Gly Gly Gly Gly Gly S 11e0r Gly Gly
Gly  Gly Ser
115 Gly Ser His Ser Met Tyr Phe Phe Thr
120 Ser Val Ser
125 Arg Pro Gly Arg Gly Glu Pro Arg Phe Ile Ala Val Gly Tyr Val Asp 130 135 140
Asp Thr Gin Phe Val Arg Phe Asp Ser Asp Ala
145 Ala Ser Gin Arg Met
150 155 160 Glu Pro Arg Ala Pro Trp Ile Glu Gin Glu Giy Prc Glu Tyr Trp Asp
165 170 175 Gly Glu Thr Arg Lys Val Lys Ala HIS Ser Gin Thr His Arg Val Asp
180 185 190
Leu Gly Thr Leu Arg Gly Tyr Tyr Asn Gin Ser Glu Ala Gly Ser His
195 200 205
Thr Val Gin Arg Met Tyr Gly Cys Asp Val Gly
210 Ser Asp Trp Arg Phe
215 220
Leu Arg Gly Tyr His Gin Tyr Ala Tyr Asp Gly Lys
225                                       Asp Tyr Ile Ala
230 235 240 Leu Lys Glu Asp Leu Arg Ser Trp Thr Ala Ala Asp Met Ala Ala Gin
245 250 255 Thr Thr Lys His Lys Trp Glu Ala Ala His Val Ala Glu Gln Leu Arg
260 265 270
Ala Tyr Leu Glu Gly Thr Cys Val Glu Trp Leu Arc Arg Tyr Leu Glu
275 280 285
Asn Gly Lys Glu Thr Leu Gin Arg Tnr Asp Ala Pro
290 Lys Thr His Met
295 300
Thr His His Ala Val Ser Asp His Glu
05 Ala Thr Leu Arg Cys Trp Ala 3 310 315 320 Leu Ser Phe Tyr Pro Ala Glu lie Thr Leu Thr Trp Gin Arg Asp Gly
325 330 335 Glu Asp Gin Thr Gin Asp Thr Glu Leu Val Glu Thr Arg Pro Ala Gly
340 345 350
Asp Gly Thr Phe Gin Lys Trp Ala Ala Val Val Val Pro Ser Gly Gin
355 360 365
Glu Gin Arg Tyr Thr Cys His Val Gin His Glu Gly Leu Pro Lys
370 375 380
Leu Thr Leu Arg Trp Glu Gin Ser Thr Arg
385                                Gly Gly Ala Ser Gly
390 395
Gly Leu Gly Gly Ile Phe Glu Ala Met Lys Met Glu Leu Arg Asp
405 410 415 <210> 6
<211> 280
<212> PRT
<213> Homo sapiens
<400> 6
Gly Ser His
1             Met Arg Tyr Phe Phe Thr Ser Val Ser Arg Pro Gly
5 10
Arg Gly Glu Arg Phe Ile Ala Val Gly Tyr Val Asp Asp Thr Gin
Phe Val Arg Asp Ser Asp Ala Ala Ser Gin Arg Met Glu Pro Arg
40
Ala Pro Trp Glu Gin Glu Gly Pro Glu Tyr Trp Asp Gly Glu Thr
55
Arg Lys Val Ala His Ser Gln Thr His Arg Val Asp Leu Gly Thr
70               75  Leu Arg Gly Tyr Tyr Asn Gin Ser Glu Ala Gly Ser His Thr Val Gin
90
Arg Met Tyr Gly Cys Asp Val Gly Ser
100 Asp Trp Arg Phe
105 Leu Arg
110 Gly Tyr His Gin Tyr Ala Tyr Asp Gly Lys Asp Tyr lle
115 120 Ala Leu Lys Glu
125
Asp Leu Arg Ser Trp Thr Ala Ala
130              135    Asp  Met Ala A
1l
40a Gin Thr Thr Lys His Lys Trp Glu
145 Ala Ala His Val Ala Glu Gin Leu Arg Ala Tyr Leu
150 155 160 Glu Gly Thr Cys Val Glu Trp Leu
165 Arg Arg Tyr Leu
170       Glu  Asn Gly
175 Lys Glu Thr Leu Gin Arg Thr Asp Ala Pro Lys Thr His Met Thr His His
180 185 190
Ala Val Ser Asp His Glu Ala Thr Leu
195 Arg Cys Trp
200 Ala Leu Ser Phe
205
Tyr Pro Ala Glu Ile Thr Leu
210 Thr rrp
215       Gin  Arg A
22s
0p Gly Glu Asp Gin Thr Gin Asp Thr
225 Glu Leu Val Glu rhr
230 Arg Pro Ala Gly
235       Asp  Gly Thr
240 Phe Gin Lys Trp Ala Ala Val Val /al
245 Pro Ser Gly Gln
250 Glu Gin Arg
255 Tyr Thr Cys His Val Gin His Glu
260 Gly Leu Pro Lys Pro Leu Thr
165 Leu
270
Arg Trp Glu Gin Ser Thr Arg Gly
275 280
<210> 7
<211> 100
<212> PRT
<213> Homo sapiens
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Met Ile Gin Arg Thr Pro
1 Lys Ile Gin
5 Val Tyr Ser Arg His
Glu  Asn Gly Lys Ser Asn Phe Leu Asn
25 Cys Tyr Val Ser Gly
Pro Ser Asp Ile Glu Val Asp Leu Leu
40 Lys Asn Gly Glu Arg
Lys Val Glu His Ser Asp Leu Ser Phe Ser
55 Lys Asp Trp Ser
Leu Leu Tyr Tyr Thr Glu Phe Thr Pro
70            Thr Glu
75 Lys Asp Glu
Cys Arg Val Asn His Val Thr Leu Ser Gin Pro Lys Ile Val
Asp  Arg Asp Met
100
<210> 8
<211> 36
<212> DNA
<213>  Artificial  -c220>
<223> synthetic oligonucleotide
<400> 8
aggagatata catatgggct ctcactccat gaggta
<210> 9
<211> 43
<212> DNA
<213> Artificial
<220>
<223> synthetic oligonucleotide
<400> 9
cgggctttgt tagcaccgat tcataggt-ja ggggcttggg caa ;3
<210>
<211>
<212> PRT
<213> Artificial
<220>
<223> linker peptide
<400>
Gly Gly Gly Gly Ser Gly Gly Gl-Y Gly Ser Gly Gly Gly
1                                             Gly Ser
5 10
<210> 11
<211>
<212> DNA
<213>. Artificial
<220>
<223> synthetic oligonucleotide
<400> 11
ggagatatac atatgatcca gcgtactcca aagat
<210> 12
<211> 49
<212> DNA
<213> Artificial
<220>
<223> synthetic oligonucleotide
< c4g0g0g>ctt 1tg2t tagcagccga attcattaca tgtctcgatc ccacttaac 49
6
<210> 13
<211> 41
<212> DNA
<213> Artificial
<220>
<223> synthetic oligonucleotide
<400> 13
ggaaggcgtt ggcgcatatg atccagcgta ctccaaagat t 41 <210> 14
<211>
<212> DNA
<213> Artificial
<220>
<223> synthetic oligonucleotide
<400> 14
ggaagcggcg gtggaggctc tggtggaggt ggcag-cggct ctcactccat
<210>
<211>
<212> DNA
<213> Artificial
<220>
<223> synthetic oligonucleotide
<400>
ggaagcggcg gtggaggctc tggtggaggt ggcagcggct ctcactccat
<210> 16
<211> 43
<212> DNA
<213> Artificial
<220>
<223> synthetic oligonucleotide
<400> 16
gggagaattc ttactcccat ctcagggtga ggggcttggg caa 43 <210> 17
<211> 14
<212> PRT
<213>  Artificial 
7
<220>
<223> specific biotinylation peptide sequence
<400> 17
Leu Gly Gly lie Phe Glu
1 Ala Met Lys Met
5 Glu Leu Arg Asp <210> 13
<211> 11
<212> PRT
<213> Artificial
<220>
<223> linker
<400> 18
Gin Ser Thr Arq Gly
1 Gly Ala Ser Gly
5 Gly Gly
<210> 19
<211> 100
<212> DNA
<213> Artificial
<220>
<223> synthetic oligonucleotide
<400>
cagtaaaa1
q9
c tttttatcag cctccgaact gtggatgcct ccacgccgaa cctccaccag aaccacctcc ggacccgcca cctccctccc atctcagggt 100 <210>
<211>- 39
<212> DNA
<213> Artificial
<220>
<223> BirA recognition tag sequence
<400>
ggaatctttg aggcaatgaa gatggagctg cgggactga 39
44
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