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1. WO1988003947 - PROTEASE ALCALINE ACTIVE A BASSE TEMPERATURE OBTENUE A PARTIR DU $i(NOCARDIOPSIS DASSONVILLEI) ET PREPARATION DE LADITE PROTEASE

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[ EN ]
CLAIMS

1. A protease-producing strain of Nocardiopsis,
characterized by having optimal pH for growth at about 9, by essentially no growth below pH 8, by having optimal
temperature for growth at 20-30°C, by essentially no growth above 35°C, and by belonging to N. dassonvillei or to the novel species defined by the strain 10R.

2. The strain of Claim 1, being N. dassonvillei M58-1 or Nocardiopsi s sp . 10R , or a mutant or variant thereof.

3. A process for preparing alkaline protease,
characterized by comprising cultivating a Nocardiopsis strain aerobically under submerged conditions containing suitable carbon and nitrogen sources at an alkaline pH, preferably pH 8-10, wherein cultivation is carried out at temperatures in the range of 20°C-30°C, thereafter recovering the enzyme from the culture broth.

4. The process of Claim 3, using the strain of Claims 1 -2.

5. A process for preparing alkaline protease,
characterized by comprising cultivation of a microorganism containing a gene encoding for and expressing a protease derived from the strain of Claims 1 - 2, thereafter
recovering the protease from the culture broth.

6. The process of Claim 5, wherein the microorganism to be cultivated is the strain of Claims 1 - 2.

7. The process of Claim 5, wherein the microorganism to be cultivated is a transformed host organism, wherein the gene has been inserted.

8. The process of Claim 7, wherein the host organism is a Nocardiopsis strain, a Streptomyces strain, a yeast or an
Aspergillus strain, preferably A. oryzae.

9. The alkaline protease preparation produced by the method of Claims 3 - 8.

10. An alkaline protease preparation derived from
Nocardiopsis, characterized by having at least 60% of its maximum activity in the pH range 7-11, measured with casein as substrate.

11. The protease preparation of Claim 10, characterized by being derived from the strain of Claims 1 - 2.

12. A detergent additive comprising alkaline protease, characterized in that the protease is produced by cultivation of a microbial strain containing a gene encoding for and expressing a protease derived from a strain of Nocardiopsis.

13. The additive of Claim 12, wherein the Nocardiopsis strain is the strain of Claims 1 - 2 or is a protease-producing strain of N. dassonvillei.

14. The additive of Claims 12 - 13, characterized by having at least 60% of its maximum activity in the pH range 7-11, measured with casein as substrate.

15. The additive of Claims 12 - 14, provided as a nondusting granulate or as a stabilized liquid.

16. The additive of Claims 12 - 15, having proteolytic activity of 0.5 - 10 CPU/g.

17. The additive of Claims 12 - 16, further comprising an alkaline Bacillus protease.

18. An enzymatic detergent composition characterized by comprising the proteolytic additive of Claims 12 - 17.

19. The composition of Claim 18, having a proteolytic activity of 0.001 - 0.5 CPU/g.