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1. WO2016094547 - FACTEURS DE TRANSCRIPTION ET PROCÉDÉ POUR AUGMENTER LA LONGUEUR DE FIBRE DE COTON

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

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Claims:

1. A seed of cotton cultivar recombinantly modified that overexpresses at least one of AtRAVl or MRAV2 or orthologs thereof to confer longer fibers to transgenic cotton plants grown under drought conditions.

2. The seed of claim 1, wherein the parent cotton cultivar is Coker 312, UA-48, Acala SJ2, Acala SJ4, Acala SJ5, Acala SJ-C1, Acala B1644, Acala B 1654-26, Acala B1654-43, Acala B3991, Acala GC356, Acala GC510, Acala GAM1, Acala CI, Acala Royale, Acala Maxxa, Acala Prema, Acala B638, Acala B1810, Acala B2724, Acala B4894, Acala B5002, non Acala picker Siokra, stripper variety FC2017, Coker 315, STONEVILLE 506, STONEVILLE 825, DP50, DP61, DP90, DP77, DES119, McN235, HBX87, HBX191, HBX107, FC 3027,

CHEMBRED Al, CHEMBRED A2, CHEMBRED A3, CHEMBRED A4, CHEMBRED Bl, CHEMBRED B2, CHEMBRED B3, CHEMBRED CI, CHEMBRED C2, CHEMBRED C3, CHEMBRED C4, PAYMASTER 145, HS26, HS46, SICALA, PIMA S6, ORO BLANCO PIMA, AXTE1, NM2302, C6TE, NM B3080, C6TE, NM B3080, AXTE 1-57, TEX E364, S196, 1900-1, 12302-4, C6TE, B7378, ATE-11, NM49-2, C6TE or NM B3080.

3. The seed of claim 1, wherein the plant further overexpresses AtABI5.

4. The seed of claim 1, wherein the modifications further comprise delayed flowering.

5. The seed of claim 1, wherein the AtRAVl or AXRAV2 orthologs are from dicotyledonous plant selected from the group consisting of soybean, cotton, canola, and potato.

6. The seed of claim 1, wherein the AtRAVl or AXRAV2 orthologs have a homology of 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99% or greater sequence identity with the AtRAVl and/or AtRAV2 genes of cotton plants.

7. A cotton plant, or a part thereof, produced by growing the seed of claim 1.

8. A tissue culture of cells produced from the plant of claim 7, wherein said cells of the tissue culture are produced from a plant part selected from the group consisting of leaves, pollen, embryos, cotyledons, hypocotyl, meristematic cells, roots, root tips, pistils, anthers, flowers, and stems.

9. A protoplast produced from the plant of claim 7.

10. A protoplast produced from the tissue culture of claim 8.

11. A cotton plant regenerated from the tissue culture of claim 8, wherein the plant has all of the morphological and physiological characteristics of conferring longer fibers to transgenic cotton plants with a yield equivalent to cotton cultivar UA-48.

12. A method for producing an Fi hybrid cotton seed, wherein the method comprises crossing the plant of claim 7 with a different cotton plant and harvesting the resultant Fi hybrid cotton seed.

13. A hybrid cotton seed produced by the method of claim 12.

14. A hybrid cotton plant, or a part thereof, produced by growing said hybrid seed of claim 13.

15. A method of producing an insect resistant cotton plant, wherein the method comprises transforming the cotton plant of claim 7 with a transgene that confers insect resistance.

16. An insect resistant cotton plant produced by the method of claim 15.

17. The cotton plant of claim 16, wherein the transgene encodes a Bacillus thuringiensis endotoxin.

18. A method of producing a disease resistant cotton plant, wherein the method comprises transforming the cotton plant of claim 7 with a transgene that confers disease resistance.

19. A disease resistant cotton plant produced by the method of claim 18.

20. A method of producing a cotton plant with longer fibers and delayed flowering to transgenic cotton plants under drought conditions, wherein the method comprises transforming the cotton plant of claim 7 with a transgene that overexpresses AtRAVl and AtRAV2 or orthologs thereof, wherein the transgenic cotton ovules produce longer lint of higher quality without significant reductions in yield.

21. A cotton plant overexpresses at least one of AtRAVl or AtRAV2 or orthologs thereof produced by the method of claim 20.

22. The cotton plant of claim 21, wherein a fiber obtained from the plant is stronger and more uniform that from the parent cultivar when both are grown under drought conditions.

23. A method of introducing a desired trait into a cotton cultivar, wherein the method comprises:

(a) crossing a first cultivar with a plant of another cotton cultivar that overexpresses at least one of AtRAVl or AtRAV2 or orthologs thereof to produce a desired trait to produce progeny plants wherein the desired trait is transgenic cotton ovules that produce longer lint of higher quality without significant reductions in yield;

(b) selecting one or more progeny plants that have the desired trait;

(c) backcrossing the selected progeny plants with the first cultivar to produce backcross progeny plants;

(d) selecting for backcross progeny plants that have the desired trait; and

(e) repeating steps (c) and (d) two or more times in succession to produce selected third or higher backcross progeny plants that comprise the desired trait and all of the physiological and morphological characteristics of the first cultivar that produce longer lint of higher quality without significant reductions in yield.

24. The cotton plant of claim 23, wherein the plant further overexpresses AtABI5.