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1. WO2020159956 - CAPTEURS D’ANALYTE ET PROCÉDÉS DE DÉTECTION PERMETTANT LA DOUBLE DÉTECTION DU GLUCOSE ET DES CÉTONES

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

[ EN ]

CLAIMS

What is claimed is the following:

1. An analyte sensor comprising :

a sensor tail comprising at least a first working electrode and a second working electrode;

a ketones-responsive active area disposed upon a surface of the first working electrode, the ketones-responsive active area comprising an enzyme system comprising at least two enzymes that are capable of acting in concert to facilitate detection of ketones;

a glucose-responsive active area comprising a glucose-responsive enzyme disposed upon a surface of the second working electrode; and

a membrane having a first portion overcoating the ketones-responsive active area and a second portion overcoating the glucose-responsive active area;

wherein the first portion and the second portion have different compositions.

2. The analyte sensor of claim 1, wherein the first portion is multi-component and comprises at least a first membrane polymer and a second membrane polymer that differ from one another.

3. The analyte sensor of claim 2, wherein the second portion is homogenous and comprises one of the first membrane polymer and the second membrane polymer.

4. The analyte sensor of claim 2, wherein the first portion comprises at least two membrane layers.

5. The analyte sensor of claim 4, wherein the first membrane polymer is

disposed directly upon the ketones-responsive active area, and the second membrane polymer is disposed upon the first membrane polymer and upon the glucose-responsive active area.

6. The analyte sensor of claim 4, wherein the first portion of the membrane is thicker than the second portion of the membrane.

7. The analyte sensor of claim 4, wherein the first portion of the membrane comprises polyvinylpyridine and polyvinylpyridine-co-styrene, and the second portion of the membrane comprises polyvinylpyridine-co-styrene.

8. The analyte sensor of claim 2, wherein the first portion of the membrane comprises an admixture of the first membrane polymer and the second membrane polymer.

9. The analyte sensor of claim 1, wherein the first portion and the second

portion define a continuous membrane overcoating the ketones-responsive active area and the glucose-responsive active area.

10. The analyte sensor of claim 1, wherein the sensor tail is configured for

insertion into a tissue.

11. The analyte sensor of claim 1, wherein the glucose-responsive active area and the ketones-responsive active area each comprise an electron-transfer agent that is covalently bonded to a polymer in each of the glucose- responsive active area and the ketones-responsive active area.

12. The analyte sensor of claim 11, wherein the glucose-responsive enzyme is covalently bonded to the polymer in the glucose-responsive active area and one or more of the at least two enzymes in the enzyme system are

covalently bonded to the polymer in the ketones-responsive active area.

13. The analyte sensor of claim 1, further comprising :

a control module in communication with the analyte sensor and an electrical system of a vehicle, the control module including a computer system programmed to receive and process data provided by the analyte sensor,

wherein operation of the vehicle is controlled or disabled by the computer system when a real-time measured analyte level of the operator crosses a predetermined safe threshold.

14. A method comprising:

exposing an analyte sensor to a fluid comprising at least one of glucose and ketones;

wherein the analyte sensor comprises a sensor tail comprising at least a first working electrode and second working electrode, a ketones- responsive active area disposed upon a surface of the first working electrode, a glucose-responsive active area disposed upon a surface of the second working electrode, and a membrane having a first portion overcoating the ketones-responsive active area and a second portion overcoating the glucose-responsive active area;

wherein the first portion and the second portion have different compositions;

wherein the glucose-responsive active area comprises a glucose-responsive enzyme, and the ketones-responsive active area comprises an enzyme system comprising at least two enzymes that are capable of acting in concert to facilitate detection of ketones;

applying a potential to the first working electrode and the second working electrode;

obtaining a first signal at or above an oxidation-reduction potential of the glucose-responsive active area, the first signal being proportional to a concentration of glucose in the fluid;

obtaining a second signal at or above an oxidation-reduction potential of the ketones-responsive active area, the second signal being proportional to a concentration of ketones in the fluid; and

correlating the first signal to the concentration of glucose in the fluid and the second signal to the concentration of ketones in the fluid.

15. The method of claim 14, wherein the first portion is multi-component and comprises at least a first membrane polymer and a second membrane polymer that differ from one another.

16. The method of claim 15, wherein the second portion is homogeneous and comprises one of the first membrane polymer and the second membrane polymer.

17. The method of claim 15, wherein the first portion comprises at least two

membrane layers.

18. The method of claim 17, wherein the first membrane polymer is disposed directly upon the ketones-responsive active area, and the second membrane polymer is disposed upon the first membrane polymer and upon the glucose- responsive active area.

19. The method of claim 17, wherein the first portion of the membrane is thicker than the second portion of the membrane.

20. The method of claim 17, wherein the first portion of the membrane

comprises polyvinylpyridine and polyvinylpyridine-co-styrene, and the second portion of the membrane comprises polyvinylpyridine-co-styrene.

21. The method of claim 15, wherein the first portion of the membrane

comprises an admixture of the first membrane polymer and the second membrane polymer.

22. The method of claim 14, wherein the glucose-responsive active area and the ketones-responsive active area each comprise an electron-transfer agent that is covalently bonded to a polymer in each of the glucose-responsive active area and the ketones-responsive active area.

23. The method of claim 22, wherein the glucose-responsive enzyme is covalently bonded to the polymer in the glucose-responsive active area and one or more of the at least two enzymes in the enzyme system are covalently bonded to the polymer in the ketones-responsive active area.

24. The method of claim 14, wherein the fluid is a biological fluid and the analyte sensor is exposed to the biological fluid in vivo.

25. The method of claim 14, wherein the first signal and the second signal are measured at different times.

26. The method of claim 14, wherein the first signal and the second signal are obtained simultaneously via a first channel and a second channel.