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1. WO2011059684 - VARIANTES DE GLYCOSYLATION DES ANTICORPS

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WHAT IS CLAIMED:

1. An Fc-containing molecule with increased resistance to protease comprising an antibody Fc domain with N-glycosylation sites at the ends of loop structures.

2. The Fc-containing molecule of claim 1 , wherein Fc domain is IgG4 and the N- glycosylation sites are in the CH3 domain.

3. The Fc-containing molecule of claim 1, wherein the N-glycosylation sites are distal from proteolytic cleavage sites.

4. The Fc-containing molecule of claim 1, wherein the Fc domain is from any of IgGl, IgG2, IgG3, and IgG4 molecule.

5. The Fc-containing molecule of claim 1, wherein the Fc-containing molecule is an

antibody or Fc fusion protein.

6. The Fc-containing molecule of claim 1 , wherein the protease is selected from the group consisting of pepsin, plasmin, trypsin, chymotrypsin, a matrix metalloproteinase, a serine endopeptidase, and a cysteine protease.

7. The Fc-containing molecule of claim 6, wherein the protease is a matrix

metalloproteinase selected from the group consisting of gelatinase A (MMP2), gelatinase B (MMP-9), matrix metalloproteinase-7 (MMP-7), stromelysin (MMP-3), and macrophage elastase (MMP-12).

8. The Fc-containing molecule of claim 1 , wherein the Fc domain exhibits N-glycosylation sites correlative to EU numbering at at least one of residues 359, 382, and 419.

9. The Fc-containing molecule of claim 8, wherein the Fc domain exhibits N-glycosylation sites correlative to EU numbering at residues 359, 382, and 419 of the Fc domain.

10. The Fc-containing molecule of claim 8, wherein the Fc domain exhibits N-glycosylation sites correlative to EU numbering at residues 359, 382, and 419 of the Fc domain, and an N-glycosylation site at residue 297 of the Fc domain is removed.

11. The Fc-containing molecule of claim 10, wherein residue 299 is changed from Thr to Asn, residue 359 is changed from Thr to Asn, residue 361 is changed from Asn to Thr, residue 419 is changed from Thr to Asn, and residue 421 is changed from Asn to Thr.

12. The Fc-containing molecule of claim 8, wherein the Fc domain has a change from wild- type at least one of residue 359, 361, 419, and 421.

13. The Fc-containing molecule of claim 12, wherein residue 359 is changed from Thr to Asn and residue 361 is changed from Asn to Thr, and/or residue 419 is changed from Thr to Asn and residue 421 is changed from Asn to Thr.

14. The Fc-containing molecule of claim 12, wherein the Fc domain has a change from wild- type at residues 359, 361, 419, and 421.

15. The Fc-containing molecule of claim 14, wherein residue 359 is changed from Thr to Asn, residue 361 is changed from Asn to Thr, residue 419 is changed from Thr to Asn, and residue 421 is changed from Asn to Thr.

16. The Fc-containing molecule of claim 1, wherein correlative to EU numbering at least one of residues 228, 234, and 235 in the hinge region is altered.

17. An Fc-containing molecule with increased resistance to protease comprising an antibody Fc domain with N-glycosylation sites correlative to EU numbering at residues 359, 382, and 419 of the Fc domain and an N-glycosylation site at residue 297 of the Fc domain is removed, wherein the Fc domain has a change from wild-type at residues 299, 359, 361, 419, and 421.

18. The Fc-containing molecule of claim 17, wherein correlative to EU numbering at least one of residues 228, 234, and 235 in the hinge region is altered.

19. A method for treating a disease characterized by the release of a protease, comprising administering to a subject or patient a glycosylated Fc-containing protein preparation, wherein the antibody preparation has residues altered from wild-type, the residues being distant from the FcyR binding site in the lower hinge or distant from the FcRn binding site at the CH2-CH3 junction region.

20. A method of increasing resistance of an Fc-containing protein to cleavage by a protease, comprising adding N-glycosylation sites to the Fc-containing protein correlative to the EU numbering at at least one of positions 359, 382, and 419.

21. The method of claim 20, comprising adding N-glycosylation sites to the Fc-containing protein correlative to the EU numbering at positions 359, 382, and 419.

22. The method of claim 21 , further comprising removing the N-glycosylation site correlative to the EU numbering at position 297.

23. A method of changing the susceptibility of an Fc-containing protein to cleavage by a protease, comprising altering N-glycosylation sites of the sequence of the Fc-containing protein correlative to the EU numbering at residues 359, 382, and 419.

24. The method of claim 23, comprising adding N-glycosylation sites to the Fc-containing protein correlative to the EU numbering at positions 359, 382, and 419.

25. The method of claim 24, further comprising removing the N-glycosylation site correlative to the EU numbering at position 297.

26. The method of claim 23, further comprising altering from wild type correlative to EU numbering at least one of residues 228, 234, and 235 in the hinge region.

27. Any invention described herein.