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1. WO2011059385 - COMPOSITION D'IMMUNISATION POUR RÉDUIRE DES INFECTIONS STREPTOCOCCIQUES

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Claims

1. An antigenic composition comprising at least one antigen, wherein said at least one antigen comprises at least part of a protein selected from EndoSe of Streptococcus equi subsp. equi and EndoSz of Streptococcus equi subsp. zooepidemicus, and wherein said at least part of said protein comprises at least one antigenic epitope.

2. The antigenic composition of claim 1, wherein the at least one antigen is selected from EndoSe comprising the amino acid sequence of SEQ ID NO: 2; EndoSe comprising the amino acid sequence of SEQ ID NO: 4; fragment A of EndoSe comprising the amino acid sequence of SEQ ID NO: 6; fragment C of EndoSe comprising the amino acid sequence of SEQ ID NO: 8; EndoSz comprising the amino acid sequence of SEQ ID NO: 11; and EndoSz comprising the amino acid sequence of SEQ ID NO: 13.

3. An antigenic composition comprising at least one antigen, wherein said at least one antigen comprises at least part of a protein selected from EndoSe of Streptococcus equi subsp. equi, EndoSz of Streptococcus equi subsp. zooepidemicus or EndoS of

Streptococcus pyogenes, and wherein said at least part of said protein comprises at least one antigenic epitope; or an analog thereof; and at least one further antigen selected from extracellular proteins, or fragments thereof, expressed extracellularly in pathogenic streptococci.

4. The antigenic composition of claim 3, wherein the extracellular proteins, or fragments thereof, comprise ONE, FNZ, ScIC, SFS, EAG, IdeE, IdeE2, Eq5, Eq8, IdeZ2, Eqz5, Eqz8 and antigenic fragments thereof.

5. The antigenic composition of claim 3 or 4, wherein the at least one antigen is selected from EndoSe comprising the amino acid sequence of SEQ ID NO: 2; EndoSe comprising the amino acid sequence of SEQ ID NO: 4; fragment A of EndoSe comprising the amino acid sequence of SEQ ID NO 6; fragment C of EndoSe comprising the amino acid sequence of SEQ ID NO: 8; EndoSz comprising the amino acid sequence of SEQ ID NO: 11; EndoSz comprising the amino acid sequence of SEQ ID NO: 13; EndoS comprising the amino acid sequence of SEQ ID NO: 15; and EndoS comprising the amino acid sequence of SEQ ID NO: 17.

6. The antigenic composition of any one of claims 1 to 5, wherein the antigenic composition is immunogenic.

7. The antigenic composition of any one of claims 1-6, wherein at least one antigen is an isolated or purified antigen.

8. The antigenic composition of any one of claims 1-7, wherein at least one antigen is recombinantly produced.

9. A vaccine composition for protecting a mammal against infection with Streptococcus equi subsp. equi and/or subsp. zooepidemicus or Streptococcus pyogenes, which comprises as immunizing component an antigenic composition comprising at least one antigen which comprises at least part of a protein selected from EndoSe of Streptococcus equi subsp. equi, EndoSz of Streptococcus equi subsp. zooepidemicus or EndoS of Streptococcus pyogenes, and wherein said at least part of said protein comprises at least one antigenic epitope; or an analog thereof; and a pharmaceutically acceptable carrier.

10. The vaccine composition of claim 9, which comprises as immunizing component an antigenic composition of any one of claims 1 to 8.

11. The vaccine composition of claim 9 or 10, which further comprises an adjuvant.

12. A vaccine composition for protecting a mammal against infection with Streptococcus equi subsp. equi and/or subsp. zooepidemicus or Streptococcus pyogenes, which comprises at least one recombinant vector and at least one polynucleotide inserted therein that encodes at least one antigen which comprises at least part of a protein selected from EndoSe of Streptococcus equi subsp. equi, EndoSz of Streptococcus equi subsp. zooepidemicus or EndoS of Streptococcus pyogenes, and wherein said at least part of said protein comprises at least one antigenic epitope, or an analog thereof, and which vector is capable of expressing said at least one antigen in vivo in a mammal susceptible to infection with

Streptococcus equi subsp. equi, and/or subsp. zooepidemicus or Streptococcus pyogenes.

13. The vaccine composition of claim 12, wherein the vector is an expression vector in the form of a plasmid or a viral vector.

14. The vaccine composition of claim 12 or 13, wherein the at least one polynucleotide is selected from polynucleotides comprising the sequences of SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 5, SEQ ID NO: 7, SEQ ID NO: 10, SEQ ID NO: 12, SEQ ID NO: 14, or SEQ ID NO: 16.

15. The vaccine composition of any one of claims 9 to 14, which is provided in a physiologically administrable form, preferably a form that is administrable by intramuscular, intradermal, subcutaneous or intranasal inoculation.

16. The vaccine composition of any one of claims 9 to 15, wherein the vaccine is capable of protecting susceptible mammals against infection with Streptococcus equi or Streptococcus pyogenes, especially horses against strangles caused by Streptococcus equi subsp. equi.

17. The vaccine composition of claim 16, which is capable of stimulating serum, mucosal and/or bronchial antibody responses directed against Streptococcus equi or pyogenes antigens in mammals susceptible to Streptococcus equi or pyogenes, especially horses.

18. A method for producing an antigen of an antigenic composition of any one of claims 1 to 8, which method comprises

(a) providing a DNA fragment encoding said antigen and introducing said fragment into an expression vector;

(b) introducing said vector, which contains said DNA fragment, into a compatible host cell;

(c) culturing said host cell provided in step (b) under conditions required for expression of the product encoded by said DNA fragment; and

(d) isolating the expressed product from the cultured host cell, and, optionally,

(e) purifying the isolated product from step (d) by a chromatographic method.

19. A method for the preparation of a vaccine composition according to any one of claims 1 to 8, which vaccine composition comprises as immunizing component an antigenic composition comprising at least one antigen which comprises at least part of a protein selected from EndoSe of Streptococcus equi subsp. equi, EndoSz of Streptococcus equi subsp. zooepidemicus and EndoS of Streptococcus pyogenes, and wherein said at least part of said protein comprises at least one antigenic epitope; or an analog thereof, especially the antigenic composition of any one of claims 1 to 8, wherein the method comprises mixing the antigenic composition with a pharmaceutically acceptable carrier.

20. Use of an antigenic composition comprising at least one antigen, wherein said at least one antigen comprises at least part of a protein selected from EndoSe of Streptococcus equi subsp. equi, EndoSz of Streptococcus equi subsp. zooepidemicus and EndoS of Streptococcus pyogenes, and wherein said at least part of said protein comprises at least one antigenic epitope; or an analog thereof, especially the antigenic composition of any one of claims 1 to 8, in the preparation of a vaccine protecting against infection with

Streptococcus equi or Streptococcus pyogenes, especially against strangles caused by subsp. equi infection in horses.

21. An antigenic composition comprising at least one antigen selected from EndoSe of Streptococcus equi subsp. equi, EndoSz of Streptococcus equi subsp. zooepidemicus and EndoS of Streptococcus pyogenes, for use in prophylactic or therapeutic treatment of infections caused by Streptococcus equi subsp. equi, Streptococcus equi subsp.

zooepidemicus or Streptococcus pyogenes.

22. A method for the production of an antiserum, which method comprises administering an antigenic composition comprising at least one antigen which comprises at least part of a protein selected from EndoSe of Streptococcus equi subsp. equi, EndoSz of Streptococcus equi subsp. zooepidemicus and EndoS of Streptococcus pyogenes, and wherein said at least part of said protein comprises at least one antigenic epitope; or an analog thereof, especially the antigenic composition of any one of claims 1 to 8, to a mammal (preferably non-human) host to produce antibodies in said host and recovering antiserum containing said antibodies produced in said host.

23. A method of prophylactic or therapeutic treatment of Streptococcus equi or

Streptococcus pyogenes infection in a mammal, especially a horse, comprising administering to said mammal an immunologically effective amount of a vaccine composition of any one of claims 9 to 17, or of an antiserum produced according to claim 22.

24. A method of protecting horses against Streptococcus equi infection, which comprises inoculating a horse subcutaneously, intradermal^, intramuscularly or intranasally with a vaccine composition of any one of claims 9 to 17 to induce an immune response against Streptococcus equi in said horse.

25. The method of claim 24, wherein an immune response in the form of IgG and/or IgA and/or IgM antibodies in serum and/or the nasopharyngeal mucus is induced in said horse.

26. An antibody preparation comprising at least one, and preferably at least two monoclonal or polyclonal antibodies, or antibody fragments, specific for an antigen of an antigenic composition comprising at least one antigen which comprises at least part of a protein selected from EndoSe of Streptococcus equi subsp. equi, EndoSz of Streptococcus equi subsp. zooepidemicus and EndoS of Streptococcus pyogenes, and wherein said at least part of said protein comprises at least one antigenic epitope; or an analog thereof, especially the antigenic composition of any one of claims 1 to 8.

27. The antibody preparation of claim 26 which is to be used prophylactically or therapeutically to provide passive immunization when administered to a mammal susceptible to infection by Streptococcus equi or Streptococcus pyogenes, or infected by Streptococcus equi or Streptococcus pyogenes.

28. A method of passive immunization which comprises administering to a mammal an antibody preparation of claim 26 or 27.

29. Use of an antigen selected from EndoSe of Streptococcus equi subsp. equi, EndoSz of Streptococcus equi subsp. zooepidemicus and EndoS of Streptococcus pyogenes, antigenic fragments thereof, analogs thereof and antigenic analog fragments, as enhancer in a vaccine composition, especially a vaccine composition against infection by Streptococcus equi or Streptococcus pyogenes.

30. Use of an antigen selected from EndoSe of Streptococcus equi subsp. equi, EndoSz of Streptococcus equi subsp. zooepidemicus and EndoS of Streptococcus pyogenes, antigenic fragments thereof, analogs thereof and antigenic analog fragments, as a diagnostic tool to determine antibody titers against the antigens in sera from mammals.

31. Use of an antigen selected from EndoSe of Streptococcus equi subsp. equi, EndoSz of Streptococcus equi subsp. zooepidemicus and EndoS of Streptococcus pyogenes, antigenic fragments thereof, analogs thereof and antigenic analog fragments, as a diagnostic tool to determine the enzymatically inhibitory activity of antibodies in sera from mammals.