Traitement en cours

Veuillez attendre...

PATENTSCOPE sera indisponible durant quelques heures pour des raisons de maintenance le mardi 26.10.2021 à 12:00 PM CEST
Paramétrages

Paramétrages

Aller à Demande

1. WO2020159542 - DISPOSITIFS DE TRI DE CELLULES

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

[ EN ]

CLAIMS

What is claimed is:

1. A cell sorting device, comprising:

a microfluidic channel to serially transport individual cells from a volume of cells along a flow path;

a sensor disposed in the microfluidic channel to distinguish between a cell to be analyzed and waste fluid; and

at least two fluid transport devices disposed within the microfluidic channel, the at least two fluid transport devices comprising:

an ejector to, responsive to detection of a cell to be analyzed, eject the cell to be analyzed from the cell sorting device; and

a waste transport device to direct the waste fluid to a waste reservoir.

2. The cell sorting device of claim 1 , wherein the waste transport device comprises at least one waste ejector to eject, through a waste orifice, the waste fluid from the cell sorting device.

3. The cell sorting device of claim 2, wherein the waste ejector operates independently of the sensor.

4. The cell sorting device of claim 2, wherein responsive to detection of a cell to be analyzed, the waste ejector is deactivated.

5. The cell sorting device of claim 2, wherein the at least one waste ejector comprises multiple waste ejectors.

6. The cell sorting device of claim 1 , wherein the waste transport device is an integrated pump disposed in the microfluidic channel to move fluid through the cell sorting device towards a waste reservoir.

7. The cell sorting device of claim 6, wherein the waste reservoir is on the same substrate as a cell reservoir.

8. The cell sorting device of claim 1 , wherein the sensor is selected from: an impedance sensor;

an optical sensor;

a flow sensor;

a thermal sensor;

a pressure sensor;

a magnetic sensor; and

a sensor to detect a marker placed on the cell to be analyzed.

9. The cell sorting device of claim 1 , wherein the sensor is disposed in a region of the microfluidic channel that has a reduced cross-sectional area.

10. A method, comprising:

passing, in serial fashion, a quantity of cells from a cell reservoir to at least one cell sorting device of a microfluidic cell analysis system; and

for each cell sorting device:

detecting cells to be analyzed;

activating a cell ejector, when a cell to be analyzed is detected, to eject the cell to be analyzed; and

directing a waste fluid to a waste reservoir.

1 1. The method of claim 10, wherein cells to be analyzed are distinguished from other cells based on at least one of:

a cell size;

a cell impedance;

a cell color;

a cell fluorescence; and

a cell light scattering response.

12. A cell sorting system, comprising:

a cell reservoir to contain a volume of cells;

multiple cell sorting devices, each cell sorting device comprising:

a microfluidic channel to serially transport individual cells from the cell reservoir along a flow path;

a sensor to distinguish between a cell to be analyzed and waste fluid;

at least two fluid transport devices disposed within the microfluidic channel, the at least two fluid transport devices comprising:

at least one cell thermal inkjet resistor to, responsive to detection by the sensor of a cell to be analyzed; activate to eject the cell to be analyzed; and

a waste thermal inkjet resistor to eject waste fluid through an orifice to a waste reservoir; and

a component controller to selectively activate the cell thermal inkjet resistor and the waste thermal inkjet resistor based on an output of the sensor.

13. The cell sorting system of claim 12, wherein the multiple sorting devices are disposed underneath the cell reservoir.

14. The cell sorting system of claim 12 wherein:

the at least one cell thermal inkjet resistor comprises multiple inkjet resistors;

each cell sorting device comprises a sensor per cell thermal inkjet resistor; and

each cell thermal inkjet resistor is individually controllable.

15. The cell sorting system of claim 14, wherein each sensor per cell thermal inkjet resistor is to determine a presence of a different type of cell.