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1. US20110173720 - Method for Modifying Sugar Chain Structure in Plant, and Plant Produced by the Method

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

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Claims

1. A method for modifying a sugar chain structure characterized either by introducing a plant transformation vector having inserted therein a gene fragment inhibiting expression of gene coding for an enzyme involved in synthesis of GDP-fucose, which is a species of sugar nucleotide, into a plant or a plant cell to carry out transformation of the plant, or, infecting a plant or a plant cell with a plant virus vector having inserted therein a gene fragment inhibiting expression of gene coding for an enzyme involved in synthesis of GDP-fucose, thereby inhibiting expression of GDP-fucose synthetase gene to reduce or inhibit fucose modification to a plant-type N-linked sugar chain in the plant,
wherein a DNA sequence of the gene coding for the enzyme, a DNA sequence corresponding to a mutant, an allele, a variant or a homolog of the DNA sequence, or a DNA sequence having at least 50% homology to the gene is used as the gene fragment.
2. The method according to claim 1, wherein the reduction or inhibition of fucose modification to the plant-type N-linked sugar chain is a reduction or an inhibition of fucose modification to a sugar chain, including a glycoprotein sugar chain, a glycolipid sugar chain, an oligosaccharide or a polysaccharide of a plant.
3. The method according to claim 1, wherein the expression of the gene coding for the enzyme involved in the synthesis of GDP-fucose, which is a species of sugar nucleotide, transcription or translation of the gene is blocked by transcriptional gene silencing (TGS), post-transcriptional gene silencing (PTGS) or virus-induced gene silencing (VIGS), thereby blocking intracellular synthesis of GDP-fucose and reducing or inhibiting fucose modification to the sugar chain.
4. The method according to claim 1, wherein the gene coding for the enzyme involved in the synthesis of GDP-fucose is a GDP-D-mannose-4,6-dehydratase (GMD) gene or a GDP-keto-6-deoxymannose-3,5-epimerase/4-reductase (GER) gene.
5. The method according to claim 1, wherein a plant virus vector is used as the vector for inducing the inhibition of the gene.
6. The method according to claim 1, wherein a plant transformation vector is used as the vector for inducing the inhibition of the gene.
7. A transgenic plant, a plant cell or a plant obtained by the method according to claim 1, wherein GDP-fucose synthetase gene expression has been inhibited and fucose modification to a sugar chain has been reduced or inhibited.
8. The transgenic plant, the plant cell or the plant according to claim 7, wherein the transgenic plant, the plant cell or the plant is a plant cell, a plant or a progeny thereof.
9. A glycoprotein, a glycopeptide, a glycolipid or a sugar chain obtained from the plant cell, the plant or the progeny thereof defined in claim 8.
10. A method for synthesizing glycoprotein in which fucose modification to a sugar chain has been reduced or inhibited characterized by using the transgenic plant defined in claim 7 as a host.