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1. WO2020161352 - MOYENS ET PROCÉDÉS POUR AMÉLIORER LA CROISSANCE ET LE RENDEMENT DE PLANTES


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INTERNATIONAL SEARCH REPORT (ISR)
Part 1:  1  2  3  4  5  6          Part 2:  A  B  C  D  E 
International application No. Applicant's or agent's file reference
PCT/EP2020/053286 APHE-008-EP-WO
International filing date (day/month/year) (Earliest) Priority Date (day/month/year)
10 February 2020 08 February 2019
Applicant
APHEA.BIO NV
FOR FURTHER ACTION: See Form PCT/ISA/220 as well as, where applicable, item 5 below.
This international search report has been prepared by this International Searching Authority and is transmitted to the applicant according to Article 18. A copy is being transmitted to the International Bureau.
It is also accompanied by a copy of each prior art document cited in this report.
1. Basis of the report
a. With regard to the language, the international search was carried out on the basis of:
the international application in the language in which it was filed.
a translation of the international application into                                          which is the language of a translation furnished for the purposes of international search (Rules 12.3(a) and 23.1(b)).
b.
This international search report has been established taking into account the rectification of an obvious mistake authorized by or notified to this Authority under Rule 91 (Rule 43.6bis(a)).
c.
With regard to any nucleotide and/or amino acid sequence disclosed in the international application, the international search was carried out on the basis of a sequence listing:
2. Certain claims were found unsearchable
3. Unity of invention is lacking
This International Searching Authority found multiple inventions in this international application, as follows:
1. claims: 1-18(partially)
A purified bacterial endophyte for improving plant growth and/or yield, characterized in that, said endophyte comprises at least one 16S nucleotide sequence at least 95% identical to SEQ ID NO:5. Purified bacterial endophyte according to claim 1, characterized in that, said endophyte is deposited with the Polish Collection of Microorganisms, under the terms of the Budapest Treaty, as Deposit ID: B/00196.
2. claims: 1-18(partially)
A purified bacterial endophyte for improving plant growth and/or yield, characterized in that, said endophyte comprises at least one 16S nucleotide sequence at least 95% identical to SEQ ID NO: 1. Purified bacterial endophyte according to claim 1, characterized in that, said endophyte is deposited with the Polish Collection of Microorganisms, under the terms of the Budapest Treaty, as Deposit ID: B/00182.
3. claims: 1-18(partially)
A purified bacterial endophyte for improving plant growth and/or yield, characterized in that, said endophyte comprises at least one 16S nucleotide sequence at least 95% identical to SEQ ID NO: 3. Purified bacterial endophyte according to claim 1, characterized in that, said endophyte is deposited with the Polish Collection of Microorganisms, under the terms of the Budapest Treaty, as Deposit ID: B/00184.
4. claims: 1-18(partially)
A purified bacterial endophyte for improving plant growth and/or yield, characterized in that, said endophyte comprises at least one 16S nucleotide sequence at least 95% identical to SEQ ID NO:4. Purified bacterial endophyte according to claim 1, characterized in that, said endophyte is deposited with the Polish Collection of Microorganisms, under the terms of the Budapest Treaty, as Deposit ID: B/00195.
5. claims: 1-18(partially)
A purified bacterial endophyte for improving plant growth and/or yield, characterized in that, said endophyte comprises at least one 16S nucleotide sequence at least 95% identical to SEQ ID NO:6. Purified bacterial endophyte according to claim 1, characterized in that, said endophyte is deposited with the Polish Collection of Microorganisms, under the terms of the Budapest Treaty, as Deposit ID: B/00197.
6. claims: 1-18(partially)
A purified bacterial endophyte for improving plant growth and/or yield, characterized in that, said endophyte comprises at least one 16S nucleotide sequence at least 95% identical to SEQ ID NO:10. Purified bacterial endophyte according to claim 1, characterized in that, said endophyte is deposited with the Polish Collection of Microorganisms, under the terms of the Budapest Treaty, as Deposit ID: B/00202.
7. claims: 1-18(partially)
A purified bacterial endophyte for improving plant growth and/or yield, characterized in that, said endophyte comprises at least one 16S nucleotide sequence at least 95% identical to SEQ ID NO:11 or 19 (Acidovorax spp.). Purified bacterial endophyte according to claim 1, characterized in that, said endophyte is deposited with the Polish Collection of Microorganisms, under the terms of the Budapest Treaty, as Deposit ID: B/00203 or B/00207.
8. claims: 1-18(partially)
A purified bacterial endophyte for improving plant growth and/or yield, characterized in that, said endophyte comprises at least one 16S nucleotide sequence at least 95% identical to SEQ ID NO:13. Purified bacterial endophyte according to claim 1, characterized in that, said endophyte is deposited with the Polish Collection of Microorganisms, under the terms of the Budapest Treaty, as Deposit ID: B/00205.
9. claims: 1-18(partially)
A purified bacterial endophyte for improving plant growth and/or yield, characterized in that, said endophyte comprises at least one 16S nucleotide sequence at least 95% identical to SEQ ID NO:14. Purified bacterial endophyte according to claim 1, characterized in that, said endophyte is deposited with the Polish Collection of Microorganisms, under the terms of the Budapest Treaty, as Deposit ID: B/00206.
10. claims: 1-18(partially)
A purified bacterial endophyte for improving plant growth and/or yield, characterized in that, said endophyte comprises at least one 16S nucleotide sequence at least 95% identical to SEQ ID NO:15. Purified bacterial endophyte according to claim 1, characterized in that, said endophyte is deposited with the Polish Collection of Microorganisms, under the terms of the Budapest Treaty, as Deposit ID: B/00213.
11. claims: 1-18(partially)
A purified bacterial endophyte for improving plant growth and/or yield, characterized in that, said endophyte comprises at least one 16S nucleotide sequence at least 95% identical to SEQ ID NO:21. Purified bacterial endophyte according to claim 1, characterized in that, said endophyte is deposited with the Polish Collection of Microorganisms, under the terms of the Budapest Treaty, as Deposit ID: B/00192.
12. claims: 1-18(partially)
A purified bacterial endophyte for improving plant growth and/or yield, characterized in that, said endophyte comprises at least one 16S nucleotide sequence at least 95% identical to SEQ ID NO: 29. Purified bacterial endophyte according to claim 1, characterized in that, said endophyte is deposited with the Polish Collection of Microorganisms, under the terms of the Budapest Treaty, as Deposit ID: B/00178.
1.
As all required additional search fees were timely paid by the applicant, this international search report covers all searchable claims.
2.
As all searchable claims could be searched without effort justifying additional fees, this Authority did not invite payment of additional fees.
3.
As only some of the required additional search fees were timely paid by the applicant, this international search report covers only those claims for which fees were paid, specifically claims Nos.: 1-18(partially)
4.
No required additional search fees were timely paid by the applicant. Consequently, this international search report is restricted to the invention first mentioned in the claims; it is covered by claims Nos.:
Remark on Protest
The additional search fees were accompanied by the applicant’s protest and, where applicable, the payment of a protest fee.
The additional search fees were accompanied by the applicant’s protest but the applicable protest fee was not paid within the time limit specified in the invitation.
No protest accompanied the payment of additional search fees.
4. Title of the invention
The text is approved as submitted by the applicant.
The text has been established by this Authority to read as follows:
5. Abstract
The text is approved as submitted by the applicant.
The text has been established, according to Rule 38.2, by this Authority as it appears in Box No. IV. The applicant may, within one month from the date of mailing of this international search report, submit comments to this Authority.
6. Drawings
a.
The figure of the drawings to be published with the abstract is Figure No. ____________
as suggested by the applicant.
as selected by this Authority, because the applicant failed to suggest a figure.
as selected by this Authority, because this figure better characterizes the invention.
b.
none of the figures is to be published with the abstract.

A. CLASSIFICATION OF SUBJECT MATTER

     A01N 63/20 (2020.01)i; A01P 21/00 (2006.01)i; C12N 1/20 (2006.01)i
According to International Patent Classification (IPC) or to both national classification and IPC

B. FIELDS SEARCHED

Minimum documentation searched (classification system followed by classification symbols):
     A01N; C12R; C12N
Documentation searched other than minimum documentation to the extent that such documents are included in the fields searched:
Electronic data base consulted during the international search (name of data base and, where practicable, search terms used):
EPO-Internal, BIOSIS, CHEM ABS Data, EMBASE, WPI Data

C. DOCUMENTS CONSIDERED TO BE RELEVANT

Category* Citation of document, with indication, where appropriate, of the relevant passages Relevant to claim No.
(1)
A
WO 2017019633 A2 (BIOCONSORTIA INC [US]) 02 February 2017 (2017-02-02)
1-18
figures 5-16; examples
claims
example 2
claim 43
claim 15
(2)
X
ANGEL VALVERDE ET AL, "Evidence of an American Origin for Symbiosis-Related Genes in Rhizobium lusitanum", APPLIED AND ENVIRONMENTAL MICROBIOLOGY,Vol. 77, No. 16, 24 June 2011 (2011-06-24), page 5665-5670,
XP055688204
1,2,4
Y
the whole document
1-18
table 2
Rhizobium lusitanum strains P3-30 (EMBL database HM852116), P2-2 (EMBL database HM852117), and P5-6 (EMBL database HM852118)
(3)
X
A. VALVERDE ET AL, "Rhizobium lusitanum sp. nov. a bacterium that nodulates Phaseolus vulgaris", INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY,Vol. 56, No. 11, 01 November 2006 (2006-11-01), page 2631-2637,
XP055688212
1,2,4
Y
abstract
1-18
table 1
R. lusitanum strain P1-7 (EMBL database AY738130)
(4)
X
LI JUAN WU ET AL, "Genetic diversity of nodulating and non-nodulating rhizobia associated with wild soybean (Glycine soja Sieb. & Zucc.) in different ecoregions of China : The comparison between G. soja and G. max rhizobia", FEMS MICROBIOLOGY ECOLOGY.,Vol. 76, No. 3, 01 June 2011 (2011-06-01), page 439-450,
XP055688210
1,2,4
Y
table 1
1-18
R. lusitanum strain CCBAU 15087 (EMBL database GU552881)
(5)
X
XUE RUI YAN ET AL, "Mesorhizobium spp. are the main microsymbionts of Caragana spp. grown in Liaoning Province of China", FEMS MICROBIOLOGY LETTERS,Vol. 271, No. 2, 01 June 2007 (2007-06-01), page 265-273,
XP055688321
1,2,4
Y
table 1
1-18
Rhizobium strain CCBAU 11182 (EMBL database EF149005)
(6)
X
CN 109055274 A (INST AGRI RESOURCES CAAS) 21 December 2018 (2018-12-21)
1,4,6-18
Y
R. calliandrae;example 1; sequence 1
1-18
(7)
X
CN 108841761 A (INST AGRI RESOURCES CAAS) 20 November 2018 (2018-11-20)
1,4,6-18
Y
R. trifolii strain 92-29;example 1; sequence 1
1-18
(8)
X
ERKO STACKEBRANDT ET AL, "Gene Sequence Phylogenies of the Family Microbacteriaceae", CURRENT MICROBIOLOGY, SPRINGER-VERLAG, NE,Vol. 55, No. 1, 05 June 2007 (2007-06-05), page 42-46,
XP019514841
1,2,4
Y
abstract
1-18
page 45, right-hand column
Leifsonia poae strain DSM 15202
Leifsonia strain BF55
Agreia bicolorata DSM 14575, Okibacterium fritillariae DSM 15271, Agromyces cerinus DSM 8595, and Agrococcus jenensis DSM 9580
(9)
X
AL-SADI A M ET AL, "Analysis of bacterial communities associated with potting media", SPRINGERPLUS, BIOMED CENTRAL LTD, LONDON, UK,Vol. 5, No. 1, 26 January 2016 (2016-01-26), page 1-5,
XP021231419
1,2,4
Y
abstract
1-18
Leifsonia lichenia strain SQU P002, Genbank KU220847
table 1
(10)
X
SUN-YOUNG AN ET AL, "Leifsonia lichenia sp. nov., isolated from lichen in Japan", JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY, THE,Vol. 55, No. 5, 01 January 2009 (2009-01-01), page 339-343,
XP055706013
1,2,4
abstract
Leifsonia lichenia strain 2Sb
(11)
X
R. ANANDHAM ET AL, "Potential plant growth promoting traits and bioacidulation of rock phosphate by thiosulfate oxidizing bacteria isolated from crop plants", JOURNAL OF BASIC MICROBIOLOGY,Vol. 48, No. 6, 01 December 2008 (2008-12-01), page 439-447,
XP055232906
1,4,6-18
Y
abstract
1-18
table 1
Leifsonia shinshuensis strain ATSB20
(12)
X
KRISTEN M. DEANGELIS ET AL, "Bacterial quorum sensing and nitrogen cycling in rhizosphere soil : Bacterial QS and rhizosphere nitrogen cycling", FEMS MICROBIOLOGY ECOLOGY.,Vol. 66, No. 2, 01 November 2008 (2008-11-01), page 197-207,
XP055707602
1,2,4
abstract
strains kmd_072 and kmd_360
(13)
X
WO 2018073454 A1 (DCM DE CEUSTER MESTSTOFFEN NV [BE] ET AL.) 26 April 2018 (2018-04-26)
1,2,4
Y
sequence 50
1-18
claims
examples
*
Special categories of cited documents:
"A"
document defining the general state of the art which is not considered to be of particular relevance
"D"
document cited by the applicant in the international application
"E"
earlier application or patent but published on or after the international filing date
"L"
document which may throw doubts on priority claim(s) or which is cited to establish the publication date of another citation or other special reason (as specified)
"O"
document referring to an oral disclosure, use, exhibition or other means
"P"
document published prior to the international filing date but later than the priority date claimed
"T"
later document published after the international filing date or priority date and not in conflict with the application but cited to understand the principle or theory underlying the invention
"X"
document of particular relevance; the claimed invention cannot be considered novel or cannot be considered to involve an inventive step when the document is taken alone
"Y"
document of particular relevance; the claimed invention cannot be considered to involve an inventive step when the document is combined with one or more other such documents, such combination being obvious to a person skilled in the art
"&"
document member of the same patent family

D. INFORMATION ON PATENT FAMILY MEMBERS

Patent document cited in search report Publication date
(day/month/year)
Patent family member(s) Publication date
(day/month/year)
WO 2017019633 A2
02 February 2017
AR 105466 A1
AU 2016297929 A1
BR 112018001522 A2
CA 2993188 A1
CN 109072168 A
EP 3325604 A2
US 2019382714 A1
WO 2017019633 A2
04 October 2017
22 March 2018
09 October 2018
02 February 2017
21 December 2018
30 May 2018
19 December 2019
02 February 2017
CN 109055274 A
21 December 2018
NONE
CN 108841761 A
20 November 2018
NONE
WO 2018073454 A1
26 April 2018
CA 3041048 A1
EP 3311669 A1
US 2020045978 A1
WO 2018073454 A1
26 April 2018
25 April 2018
13 February 2020
26 April 2018
Name and mailing address of the ISA/:
European Patent Office
P.B. 5818, Patentlaan 2, 2280 HV Rijswijk,
Netherlands
Telephone No. (+31-70)340-2040
Facsimile No. (+31-70)340-3016
Date of the actual completion of the international search:
23 June 2020
Date of mailing of the international search report:
06 July 2020
Authorized officer:
Galley, Carl
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