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1. WO2020159538 - SYSTÈMES D'ANALYSE CELLULAIRE

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

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CLAIMS

What is claimed is:

1. A cell analysis system, comprising:

at least one cell analysis device, each cell analysis device comprising: a channel to serially feed individual cells from a volume of cells into a lysing chamber;

at least one feedback-controlled lysing element in the lysing chamber to agitate a cell;

a controller to analyze the cell, the controller comprising:

a lysate analyzer to analyze properties of a lysate of the cell; and a rupture analyzer to analyze parameters of an agitation when a cell membrane ruptures.

2. The cell analysis system of claim 1 , wherein the lysing chamber further comprises:

a sensor to determine whether the cell membrane was ruptured; and a return pump to re-direct a cell to the lysing chamber when the cell membrane has not ruptured.

3. The cell analysis system of claim 1 , wherein each cell analysis device further comprises an encapsulator to encapsulate the lysate.

4. The cell analysis system of claim 1 , further comprising:

a cell reservoir fluidically coupled upstream of each cell analysis device to hold the volume of cells; and

a waste reservoir fluidically coupled downstream of each cell analysis device to collect waste fluid.

5. The cell analysis system of claim 1 , wherein each cell analysis device further comprises an ejector to eject the lysate.

6. The cell analysis system of claim 5, wherein each cell analysis device further comprises a lysate sensor to:

detect a presence of the lysate; and

activate the ejector based on a detected presence of the lysate.

7. The cell analysis system of claim 1 , wherein each cell analysis device further comprises a cell presence sensor to:

detect a presence of a cell to be lysed in the cell analysis device; and activate the at least one feedback-controlled lysing element based on a detected presence of the cell to be lysed.

8. A cell analysis system, comprising:

a cell reservoir to hold a volume of cells to be analyzed;

a microfluidic cell analysis die comprising:

at least one cell analysis device formed in a substrate, each cell analysis device comprising:

a channel to serially feed individual cells from a volume of cells into a lysing chamber;

at least one feedback-controlled lysing element in the lysing chamber to agitate a cell;

an ejector to, responsive to a determination that a cell membrane has ruptured, eject a lysate of the cell; and a controller to analyze the cell, the controller comprising:

a lysate analyzer to analyze properties of the lysate; and a rupture analyzer to analyze parameters of an agitation when a cell membrane ruptures;

a pump to move the cells through the at least one cell analysis device; and

a waste reservoir to collect waste fluid.

9. The cell analysis system of claim 8, wherein the microfluidic cell analysis die is disposed under the cell reservoir and the waste reservoir.

10. The cell analysis system of claim 8, wherein at least one of the waste reservoir and the pump are formed in the microfluidic cell analysis die.

1 1. The cell analysis system of claim 8, further comprising a sorter to separate the cells to be analyzed from a carrier fluid.

12. The cell analysis system of claim 8, further comprising a detector of a marker of the cells to be analyzed, wherein:

the marker is formed on the cells to be analyzed; and

an output of the detector selectively activates the feedback-controlled lysing element.

13. A method, comprising:

passing, a quantity of cells from a cell reservoir to at least one cell analysis device of an underlying microfluidic cell analysis die;

for each cell analysis device, activating a feedback-controlled lysing element in a lysing chamber of the cell analysis device, wherein the feedback-controlled lysing element is to agitate the cell;

responsive to a determination that a cell membrane has ruptured: passing lysate information to a lysate analyzer; and passing parameters of an agitation when a cell membrane ruptures to a rupture analyzer; and

analyzing the cell based on output of both the rupture analyzer and the lysate analyzer.

14. The method of claim 13, further comprising incrementally adjusting agitation intensity until the cell membrane ruptures.

15. The method of claim 13, wherein:

the quantity of cells are genetic cells;

the lysate is a nucleic acid; and

the method is to sequence the genetic cells.