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1. WO2000031260 - GLYCOPROTEINE ZPEP10 A SPECIFICITE TESTICULAIRE

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

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CLAIMS

What is claimed is :

1 . An isolated polypeptide comprising an extracellular domain, wherein said extracellular domain comprises amino acid residues 22 to 111 of the amino acid sequence of SEQ ID NO : 2 .

2 . An isolated polypeptide according to claim 1 , wherein said polypeptide further comprises a transmembrane domain that resides in a carboxyl -terminal position relative to said extracellular domain, wherein said transmembrane domain comprises amino acid residues 112 to 133 of the amino acid sequence of SEQ ID NO : 2 .

3 . An isolated polypeptide according to claim 2 , wherein said polypeptide further comprises a cytoplasmic domain that resides in a carboxyl -terminal position relative to said transmembrane domain , wherein said cytoplasmic domain comprises amino acid residues 134 to 142 of the amino acid sequence of SEQ ID NO : 2 .

4 . An isolated polypeptide according to claim 2 , wherein said polypeptide further comprises a secretory signal that resides in an amino- terminal position relative to said extracellular domain, wherein said secretory signal sequence comprises amino acid residues 1 to 20 of the amino acid sequence of SEQ ID NO : 2 .

5 . An isolated polypeptide according to claim 1 comprising amino acid residue 1 to amino acid residue 142 of SEQ ID NO : 2 .

6 . An isolated polypeptide according to claim 1 , covalently linked amino terminally or carboxy terminally to a moiety selected from the group consisting of affinity tags, toxins, radionucleotides, enzymes and f luorophores.

7. An isolated polypeptide comprising a sequence of amino acid residues that is at least 80% identical to a amino acid residue 21 to amino acid residue 142 of SEQ ID NO: 2, wherein said polypeptide specifically binds with an antibody that specifically binds with a polypeptide having the amino acid sequence of SEQ ID NO: 2.

8. An isolated polypeptide according to claim 7, wherein any difference between said amino acid sequence of said isolated polypeptide and said corresponding amino acid sequence of SEQ ID NO: 2 is due to a conservative amino acid substitution.

9. An isolated polypeptide of claim 7, wherein the amino acid percent identity is determined using a FASTA program with ktup=1, gap opening penalty=10, gap extension penalty=1, and substitution matrix=blosum62, with other parameters set as default.

10. An isolated polypeptide comprising the amino acid sequence of amino acid residue 1 to amino acid residue 20 of SEQ ID NO: 2.

11. An isolated polypeptide selected from the group consisting of:

a) amino acid residues 21-111 of SEQ ID NO: 2;

b) amino acid residues 112-133 of SEQ ID NO: 2;

c) amino acid residues 134-142 of SEQ ID NO: 2;

d) amino acid residues 1-20 of SEQ ID NO : 2 ; e) amino acid residues 21-133 of SEQ ID NO: 2;

f) amino acid residues 112-142 of SEQ ID NO: 2;

g) amino acid residues 1-111 of SEQ ID NO: 2; and h) amino acid residues 1-133 of SEQ ID NO: 2.

12. A fusion protein consisting of a first portion and a second portion joined by a peptide bond, said first portion comprising a polypeptide according to claim 1, and

said second portion comprising another polypeptide.

13. A polypeptide according to claim 1 in combination with a pharmaceutically acceptable vehicle.

14. An antibody that specifically binds to an epitope of a polypeptide of claim 1.

15. An antibody according to claim 14, wherein said antibody is selected from the group consisting of:

a) polyclonal antibody;

b) murine monoclonal antibody;

c) humanized antibody derived from b) ; and d) human monoclonal antibody.

16. An antibody fragment according to claim 14, wherein said antibody fragment is selected from the group consisting of F(ab') , F(ab), Fab', Fab, Fv, scFv, and minimal recognition unit.

17. An anti-idiotype antibody that specifically binds to said antibody of claim 14.

18. A binding protein that specifically binds to an epitope of a polypeptide of claim 1.

19. A method of producing an antibody to a polypeptide comprising:

inoculating an animal with a polypeptide according to claim 1;

wherein said polypeptide elicits an immune response in the animal to produce the antibody; and

isolating the antibody f rom the animal .

20 . An isolated polynucleotide encoding a polypeptide comprising an extracellular domain , wherein said extracellular domain comprises amino acid residues 22 to 111 of the amino acid sequence of SEQ ID NO : 2 .

21 . An isolated according to claim 20 , wherein said polypeptide further comprises a transmembrane domain that resides in a carboxyl -terminal position relative to said extracellular domain , wherein said transmembrane domain comprises amino acid residues 112 to 133 of the amino acid sequence of SEQ ID NO : 2 .

22 . An isolated polynucleotide according to claim

21 , wherein said polypeptide further comprises a cytoplasmic domain that resides in a carboxyl -terminal position relative to said transmembrane domain, wherein said cytoplasmic domain comprises amino acid residues 134 to 142 of the amino acid sequence of SEQ ID NO : 2 .

23 . An isolated polynucleotide according to claim

22 , wherein said polypeptide further comprises a secretory signal that resides in an amino-terminal position relative to said extracellular domain , wherein said secretory signal sequence comprises amino acid residues 1 to 20 of the amino acid sequence of SEQ ID NO : 2 .

24 . An isolated polynucleotide according to claim 20 encoding a polypeptide compri sing amino acid residue 1 to amino acid residue 142 of SEQ I D NO: 2.

25 . An isolated polynucleotide according to claim 20 , wherein said polypeptide is covalently linked amino terminally or carboxy terminally to a moiety selected from the group consisting of affinity tags , toxins , radionucleotides , enzymes and fluorophores .

26 . An isolated polynucleotide encoding a polypeptide comprising a sequence of amino acid residues that is at least 80% identical to a amino acid residue 21 to amino acid residue 142 of SEQ ID NO : 2 , wherein said polypeptide specifically binds with an antibody that specifically binds with a polypeptide having the amino acid sequence of SEQ I D NO : 2 .

27 . An isolated polynucleotide according to claim 26, wherein any difference between said amino acid sequence of said isolated polypeptide and said corresponding amino acid sequence of SEQ ID NO : 2 is due to a conservative amino acid substitution .

28 . An isolated polynucleotide of claim 27 , wherein the amino acid percent identity is determined using a FASTA program with ktup=1 , gap opening penalty=10 , gap extension penalty=1, and substitution matrix=blosum62 , with other parameters set as default .

29 . An isolated polynucleotide selected from the group consisting of :

a ) a sequence of nucleotides from nucleotide 139 to nucleotide 411 of SEQ ID NO: 1;

b) a sequence of nucleotides from nucleotide 139 to nucleotide 477 of SEQ ID NO: 1;

c ) a sequence of nucleotides from nucleotide 139 to nucleotide 504 of SEQ ID NO: 1;

d) a sequence of nucleotides f rom nucleotide 7 9 to nucleotide 504 of SEQ ID NO: 1;

e ) a sequence of nucleotides from nucleotide 1 to nucleotide 1094 of SEQ ID NO: 1;

f) a polynucleotide that remains hybridized following stringent wash conditions to a polynucleotide consisting of the nucleotide sequence of SEQ ID NO: 1, or the complement of SEQ ID NO: 1; and

g) nucleotide sequences complementary to a), b), c), d), e), or f.

30. An isolated polynucleotide encoding a fusion protein consisting of a first portion and a second portion joined by a peptide bond, said first portion comprises a polypeptide according to claim 1; and

said second portion comprising another polypeptide.

31. An isolated polynucleotide encoding a fusion protein comprising a secretory signal sequence having the amino acid sequence of amino acid residues 1-20 of SEQ ID NO: 2, wherein said secretory signal sequence is operably linked to an additional polypeptide.

32. An isolated polynucleotide comprising the sequence of nucleotide 1 to nucleotide 426 of SEQ ID NO: 3.

34. An expression vector comprising the following operably linked elements:

a transcription promoter;

a DNA segment encoding a polypeptide according to claim 1; and

a transcription terminator.

35. An expression vector according to claim 34, wherein said DNA segment encodes a polypeptide covalently linked amino terminally or carboxy terminally to an affinity tag.

36 . An expression vector according to claim 34 wherein said DNA segment further encodes a secretory signal sequence operably linked to said polypeptide .

37 . An expression vector according the claim 34 , wherein said secretory signal sequence comprises residues 1 to 20 of SEQ ID NO : 2 .

38 . A cultured cell into which has been introduced an expression vector according to claim 34 ;

wherein said cell expresses said polypeptide encoded by said DNA segment .

39 . A method of producing a polypeptide comprising : culturing a cell into which has been introduced an expression vector according to claim 34 ;

whereby said cell expresses said polypeptide encoded by said DNA segment ; and

recovering said expressed polypeptide .