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1. WO2020154812 - SYSTÈME ET PROCÉDÉ DE DÉTERMINATION D'UN INDICE DE RÉFRACTION D'UN MILIEU

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

[ EN ]

WHAT IS CLAIMED IS:

1. A method for determining a refractive index of a medium, the method comprising:

providing a substrate having a first surface opposite to a second surface, the first surface having a first surface portion and a second surface portion spaced-apart from the first surface portion and recessed of a given depth relative to the first surface portion;

receiving the medium at least on the second surface portion;

propagating a first optical beam towards the first surface portion and a second optical beam towards the second surface portion, the first and second optical beams having power within a spectral band at a given wavelength;

collecting the first and second optical beams after said propagating and generating first and second signals being indicative of a phase of a respective one of the first and second collected optical beams; and

determining a refractive index of said medium based on the first and second signals, the given depth, the given wavelength and a refractive index of the substrate.

2. The method of claim 1 wherein the medium surrounds a biological sample received on the first surface of the substrate, the method further comprising determining at least one of a refractive index and a geometry of the biological sample based on the previously determined refractive index of the medium surrounding the biological sample.

3. The method of claim 2 further comprising depositing a coating layer on the first surface of the substrate, the coating layer being made of a biologically-compatible material, the biological sample being indirectly received on the first surface of the substrate via the coating layer.

4. The method of claim 1 wherein the first and second optical beams are portions of a same optical beam.

5. The method of claim 1 wherein said first and second optical beams are propagated through the substrate prior to said collecting.

6. The method of claim 1 wherein the first and second optical beams are in phase relative to one another prior to reaching said substrate.

7. The method of claim 1 wherein the first and second optical beams are orthogonal to the substrate.

8. The method of claim 1 further comprising associating the determined refractive index of the medium to coordinates of the substrate.

9. The method of claim 8 further comprising repeating the method for a plurality of other surface portions of the first surface of the substrate, thereby associating a plurality of determined refractive indexes to respective coordinates of the substrate to form a refractive index map.

10. The method of claim 1 wherein said collecting further comprises collecting a reference beam simultaneously to said first and second optical beams, said first and second signals each being indicative of an intensity of an interference between the reference beam and a respective one of the first and second collected optical beams at corresponding ones of the detectors of the array.

11. The method of claim 1 wherein said medium is a fluid medium, said receiving comprising flowing said medium on the first and second surface portions of the substrate while performing said method.

12. A system for determining a refractive index of a medium, the system comprising:

a substrate having a first surface opposite to a second surface, the first surface having a first surface portion and a second surface portion spaced-apart from the first surface portion and recessed of a given depth relative to the first surface portion, at least the second surface portion receiving the medium;

an optical source propagating a first optical beam towards the first surface portion, and a second optical beam towards the second surface portion, the first and second optical beams having power within a spectral band at a given wavelength;

a detector collecting the first and second optical beams after said propagating and generating first and second signals being indicative of a phase of a respective one of the first and second collected optical beams; and

a computer determining a refractive index of said medium based on the first and second signals, the given depth, the given wavelength and a refractive index of the substrate.

13. The system of claim 12 wherein the substrate is a coverslip, the second surface portion being recessed from an original surface of the coverslip.

14. The system of claim 12 further comprising an enclosure having an optically transparent base, a plurality of lateral walls extending from the optically transparent base towards an optically transparent top, and a sealed cavity within the enclosure, the medium being received in the cavity, the substrate being made integral to at least one of the optically transparent base and the optically transparent top of the enclosure, the second surface portion facing towards the sealed cavity.

15. The system of claim 12 wherein the enclosure has an input port and an output port, the system further comprises a pump flowing the medium into and out of the cavity via said input and output ports.

16. The system of claim 12 wherein the system is provided in the form of a digital holographic microscopy system.

17. A substrate for use in determining a refractive index of a medium, the substrate comprising a first surface opposite to a second surface, the first surface having a first surface portion and a second surface portion spaced-apart from the first surface portion and recessed of a given depth relative to the first surface portion, wherein, during use, at least the second surface portion receives the medium, the first surface portion receives a first optical beam and the second surface portion receives a second optical beam, the first and second optical having power within a spectral band at a given wavelength l, the depth d being given by a relation equivalent to the following relation: d > l/1.21 , wherein d denotes the given depth and l denotes the wavelength of the first and second optical beams expressed in nanometers.

18. The substrate of claim 17 wherein the given depth d is given by a relation equivalent to the following relation: d > l/0.74, the refractive index being determinable with a precision of at least 0.0006.

19. The substrate of claim 17 wherein the given depth d is given by a relation equivalent to the following relation: d > l/0.41 , the refractive index being determinable with a precision of at least 0.0003.

20. The substrate of claim 17 wherein the first and second surface portions are part of a groove recessed in the first surface of the substrate.

21. The substrate of claim 17 further comprising a coating layer on the first surface of the substrate.

22. The substrate of claim 21 wherein the coating layer is made of a biologically-compatible material.