Traitement en cours

Veuillez attendre...

Paramétrages

Paramétrages

Aller à Demande

1. AU2020213921 - Immunoglobulin binding proteins for affinity purification

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

[ EN ]
CLAIMS
1. An Ig binding protein comprising one or more Ig binding domains, wherein at least one domain comprises or consists of an amino acid sequence of any one of SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10, or an amino acid sequence with at least 95 % identity to any of SEQ ID NO: 1, 2, 3, 4, 5, 6,7, 8,9, or 10.
2. The Ig binding protein according to claim 1, wherein said domain comprises the amino acid sequence as set forth in SEQ ID NO: 1 or a sequence at least 95% identical thereto.
3. The Ig binding protein according to claim 1, wherein said domain comprises the amino acid sequence as set forth in SEQ ID NO: 7 or a sequence at least 95% identical thereto, or wherein said domain comprises the amino acid sequence as set forth in SEQ ID NO: 10 or a sequence at least 95% identical thereto.
4. The Ig binding protein according to claim 3, wherein said domain comprises the amino acid sequence as set forth in SEQ ID NO: 8 or SEQ ID NO: 9.
5. The Ig binding protein according to any one of claims 1-4, wherein the protein comprises 2, 3, 4, 5, 6, 7, or 8 domains linked to each other.
6. The Ig binding protein according to claim 5, wherein the protein is a homo-multimer or a hetero-multimer.
7. The Ig binding protein according to claim 6, wherein one or more domains are linked to each other directly or with one or more linkers.
8. The Ig binding protein according to any one of claims 1-7, wherein said protein binds to IgG 1, IgG 2 , IgG 4 , IgM, IgA, Ig fragments, Fc fragments, or Fab fragments.
9. The Ig binding protein according to any one of claims 1-8, wherein the protein is immobilized to a solid support.
10. An affinity separation matrix comprising the Ig binding protein according to any one of claims 1-9 coupled to said affinity separation matrix.
11. Use of the Ig binding protein according to any one of claims 1-9 or of the affinity separation matrix according to claim 10 for affinity purification of any protein with affinity
to the Ig binding protein.
5 12. A method of affinity purification of a protein comprising an Ig sequence, the method comprising:
(a) providing a liquid that contains protein comprising an Ig sequence;
(b) providing an affinity separation matrix according to claim 10;
(c) contacting said affinity separation matrix with the liquid under conditions that LO permit binding of the at least one Ig binding protein according to any one of claim
1-9 to a protein comprising an Ig sequence; and
(d) eluting said protein comprising an Ig sequence from said affinity purification matrix.
L5 13. The method according to claim 12 wherein in step (d) wherein more than 90 % of the protein comprising the Ig sequence is eluted from the Ig binding protein according to any of claims 1-9.
14. The method according to any of claims 12-13, comprising the additional step of (e) .0 cleaning the affinity purification matrix with an alkaline cleaning liquid.
15. The method according to claim 14, wherein the Ig-binding capacity of the Ig binding protein is at least 70 % of the Ig binding capacity before the incubation under alkaline conditions.
!5