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1. WO2003022769 - SUBSTRATS MODIFIES POUR LA FIXATION DE BIOMOLECULES

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

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CLAIMS

1. A substrate for attachment of biomolecules comprising:
a first coating of Siθ2 and thereover a coating of a multiamino organosilane.

2. A substrate according to claim 1, wherein the S1O2 coating is applied to the substrate by chemical vapor deposition, sputtering, dip coating in a sol-gel, ion beam deposition, flame hydrolysis deposition, laser pyrolysis deposition, liquid phase deposition, electron beam deposition, plasma arc deposition or flash evaporation deposition.

3. A substrate according to claim 1, wherein the substrate is a low self-fluorescent glass.

4. A substrate according to claim 3, wherein the glass is borosilicate or soda lime silicate glass.

5. A substrate according to claim 1, wherein the multiamino organosilane is a multiamino alkyl monoalkoxy silane, multiamino alkyl dialkoxy silane, and/or a multiamino alkyl trialkoxy silane.

6. A substrate according to claim 1, wherein the multiamino organosilane is N-(2-amino-ethyl)-3-aminopropyl-trimethoxysilane (AEA), N-(2-aminoethyl)-3- aminopropyltrimethoxysilane (EDA), and/or (aminoethyl
aminomethyl)phenethyltrimethoxysilane (PED A) .

7. A substrate according to claim 1, wherein the multiamino organosilane is trimethoxysilylpropyl-diethylenetriamine.

8. A substrate according to claim 1, wherein the SiO2 coating is derived from tetraethylorthosilicate, tetramethylorthosilicate, sodium silicate solution, alkali silicate solution, alkaline earth silicate solution or a colloidal silica suspension.

9. An array of immobilized biomolecules comprising a plurality of biomolecules attached to a substrate according to claim 1.

10. An array of immobilized nucleic acid molecules comprising a plurality of nucleic acid molecules attached to a substrate according to claim 1.

11. A method of performing a hybridization assay comprising incubating target nucleic acid molecules with a substrate according to claim 9, wherein the biomolecules are nucleic acid oligomers.

12. An array according to claim 9, wherein the biomolecules are DNA, unmodified nucleic acid, antibodies, antigens, proteins, or oligonucleotides.

13. A method of attaching biomolecules to a substrate comprising:
coating a substrate with SiO2, thereafter applying a coating of a multiamino organo silane, and bonding biomolecules thereto.

14. A method according to claim 13, wherein the SrQ2 coating is applied to the substrate by chemical vapor deposition, sputtering, dip coating in a sol-gel, ion beam deposition, flame hydrolysis deposition, laser pyrolysis deposition, liquid phase deposition, electron beam deposition, plasma arc deposition or flash evaporation deposition.

15. A method according to claiml3, wherein the substrate is a low self-fluorescent multi-oxide component glass.

16. A method according to claim 13, wherein the substrate is a low self-fluorescent borosilicate or soda lime silicate glass.

17. A method according to claim 13, wherein the multiamino organosilane is a multiamino alkyl monoalkoxy silane, multiamino alkyl dialkoxy silane, and/or a multiamino alkyl trialkoxy silane.

18. A method according to claiml3, wherein the multiamino organosilane is trimethoxysilylpropyl-diethylenetriamine (DETA) , N-(2-aπιmo-ethyl)-3-aminopropyl- trimethoxysilane (AEA), N-(2-aminoethyl)-3-aminopropyltrimethoxy silane (EDA), and/or (aminoethyl aminomethyl)phenethyltrimethoxysilane (PEDA).

19. A method according to claim 13, wherein the SiO2 coating is derived from tetraethylorthosilicate, tetramethylorthosilicate, sodium silicate solution, alkali silicate solution, alkaline earth silicate solution or a colloidal silica suspension.

20. A method according to claim 13, wherein the attachment of biomolecules creates an array of immobilized nucleic acid molecules.

21. A substrate for attachment of biomolecules comprising:
a low self-fluorescence glass substrate having an acid leached surface
coated thereover with a multiamino organo silane.

22. A substrate according to claim 21, wherein the substrate is a low self-fluorescent multi-oxide component glass.

23. A substrate according to claim 21, wherein the multiamino organosilane is a multiamino alkyl monoalkoxy silane, multiamino alkyl dialkoxy silane, and/or a multiamino alkyl trialkoxy silane.

24. A substrate according to claim 21, wherein the multiamino organosilane is trimethoxy silylpropyl-diemylenetriamine (DETA) , N-(2-amino-ethyl)-3-aminopropyl-trimethoxy silane (AEA), N-(2-aminoethyl)-3-aminopropyltrimethoxysilane (EDA), and/or (aminoethyl aminomethyl)phenethyltrimethoxysilane (PEDA).

25. A substrate according to claim 21, wherein low self-fluorescent multi-oxide component glass is soda-lime glass or borosilicate.

26. An array of immobilized nucleic acid molecules comprising a plurality of nucleic acid molecules attached to a substrate according to claim 21.

27. A method of performing a hybridization assay comprising incubating target nucleic acid molecules with a substrate according to claim 26.

28. A substrate for attachment of biomolecules comprising a multioxide low fluorescent glass coated with a multiamino organosilane.

29. A substrate according to claim 28, wherein said glass comprises, in % by

weight on an oxide basis:

SiO2 58-85,



ZnO 0 - 8,
CaO 0 - 8,
MgO 0 - 8,


Sb2O3 0-2.

30. A substrate according to claim 28, wherein said glass comprises, in % by weight

on an oxide basis:

SiOa 40 - 60



BaO 20-30



ZnO 0-7
CaO 0-8
MgO 0-5

Sb2O3 0-2.

31. A substrate according to claim 28, wherein said glass comprises, in % by weight

on an oxide basis:

SiO2 60-70



ZnO 3-10 CaO 0-5
MgO 0-5

Sb2O3 0-2.

32. A substrate according to claim 28, wherein said glass comprises, in % by weight on

an oxide basis:

SiO2 65-75


KaO 5-15
ZnO 2-6
TiO2 0.1-5
BaO 0.1-5
CaO 0-10
MgO 0-6
PbO 0-3
AbOs 0-3



Sb2O3 0-2.

33. A substrate according to claim 28, wherein the multiamino organosilane is a

multiamino alkyl monoalkoxy silane, multiamino alkyl dialkoxy silane, and/or a

multiamino alkyl trialkoxy silane.

34. A substrate according to claim 28, wherein the multiamino organosilane is

trimethoxysilylpropyl-diethylenetriamine (DETA) , N-(2-amino-ethyl)-3-aminopropyl- trimethoxy silane (AEA), N-(2-ammoethyl)-3-aminopropyltrimethoxysilane (EDA),

and/or (aminoethyl aminomethyl)phenethyltrimethoxysilane (PEDA).

35. An array of immobilized nucleic acid molecules comprising a plurality of nucleic acid molecules attached to a substrate according to claim 28.

36. A method of performing a hybridization assay comprising incubating target nucleic acid molecules with a substrate according to claim 35.

37. A substrate for attachment of biomolecules comprising:
a multioxide low fluorescent glass having
a first coating of Siθ2 and thereover a coating of an amino silane.

38. A substrate according to claim 37, wherein said amino silane is aminopropyl silane.

39. A substrate according to claim 38, wherein said amino silane is an aminopropyl trialkoxy silane, aminobutyldimethylmethoxy silane.

40. A substrate according to claim 37, wherein said amino silane is gamma-3-amino propyl triethoxy silane, and/or 3-amino propyl trimethoxy silane.

41. A substrate according to claim 3, wherein the substrate further comprises a barcode.