Traitement en cours

Veuillez attendre...

Paramétrages

Paramétrages

Aller à Demande

1. WO1995008640 - PROCEDE ELECTROPHORETIQUE D'ISOLATION ET DE SEPARATION DE MICROORGANISMES

Note: Texte fondé sur des processus automatiques de reconnaissance optique de caractères. Seule la version PDF a une valeur juridique

[ EN ]

We Claim :
1. A method of sorting biological cell
populations, comprising:
(a) injecting a mixture of biological cell populations into one end of a cell separator being comprised of:
(i) a small diameter capillary
separation tube having a chargeable
material distributed along the
inner surface of said tube wherein
said chargeable material is capable
of maintaining and electrical
charge;
(ii) an anodic electrode positioned at a
first end of said tube;
(iii) a cathodic electrode positioned at
a second end of said tube;
(iv) an electrolyte solution filling
said tube and contacting both said
anodic said cathodic electrodes
wherein said electrodes are
maintained in electrical
communication with each other and
said tube;
(b) applying an electrical potential of between about 1 and 40Kv across said electrodes whereby both an electrophoretic force and an electroosmotic force is produced within said electrolyte solution; and (c) allowing migration of biological cells to proceed.
2. A method according to Claim 1 wherein said chargeable material is silica.
3. A method according to Claim 1 wherein said biological cells are bacteria.

4. A method according to Claim 1 wherein the charge of said chargeable material is determined
independently of the charge of said electrolyte
solution.
5. A method according to Claim 1 wherein said capillary tube has a length from about 50 to 1500 cm and a diameter from about 25 to 150 microns .
6. A method of sorting biological cell
populations comprising the steps of:
(i) providing a cell collecting system
further comprising:
(a) a movable fraction dispensing device
capable of dispensing small volume
fractions;
(b) a solid growth medium capable of
supporting the growth of biological
cells;
(c) a detector means operably connected
to said dispensing device and capable
of detecting the presence of microorganism in said dispensing device;
and
(d) a computer for the coordination of
said detector and said dispensing
device;
(ii) moving said dispensing device in a
specific pattern above said growth
medium in response to the detection of
biological cells in said device;
(iii) collecting said fractions containing
said biological cells on said solid
growth medium;
(iv) incubating said growth medium for a
time sufficient to produce individual
colonies of biological cells;

(v) analyzing said colonies for the
presence of specific biological cells.

7. An apparatus for the separation of biological cell populations comprising:
(a) a small diameter capillary separation tube
having a layer of chargeable material
distributed along the inner surface of
said tube wherein said chargeable material
is capable of maintaining an electrical
charge;
(b) an anodic electrode positioned at a first
end of said tube;
(c) a cathodic electrode positioned at a
second end of said tube;
(d) an electrolyte solution filling said tube
and contacting said anodic and said
cathodic electrodes.
8. A method according to Claim 1 wherein said electrical potential across said electrodes produces both an electrophoretic and an electroosmotic force within said electrolyte solution.
9. A method according to Claim 8 wherein said electroosmotic force may be varied in a controlled fashion.
10. An apparatus for the electrophoretic
separation of Biological cell populationscomprising:
(i) a small diameter capillary separation
tube further comprising a chargeable
material distributed along the inner
surface of said tube wherein said
material is capable of maintaining an
electrical charge;
(ii) an anodic electrode positioned at a
first end of said tube;

(iii) a cathodic electrode positioned at a
second end of said tube;
(iv) an electrolyte solution filling said
tube and contacting both the anodic and 5 cathodic electrodes wherein said
electrodes are maintained in electrical
communication with each other and with
said tube and wherein said electrolyte
moves and is eluted from either the 10 first end or the second end of said
capillary tube under the influence of
an electric field;
(v) a high voltage power supply
electrically connected to said anodic 15 electrode and said cathodic cathode,
said power supply capable of generating
an electric potential across said
electrodes of from about lkv to about
40kv;
20 (vi) a robotic dispensing tip means for the
controlled dispensing of said eluted
electrolyte solution wherein said
dispensing tip is firmly affixed to
either said first or said second end of 25 said capillary tube;
(vii) a fraction collecting means for
receiving said eluted electrolyte
solution;
(viii) a transport means positioned just below 30 said dispensing tip for the controlled
movement of said faction collecting
means;
(ix) a detector for the detection of cell
movement within said capillary;

(x) a data collection means for collecting
and recording data generated by said
detector;and
(xi) a computer for the analysis of said 5 data and for the coordination of the
movement of said robotic transport
means .