Colecciones nacionales e internacionales de patentes

1. (WO2018087644) IMMUNOGENIC GIANT EXTRACELLULAR VESICLES (VEGS) OF PARASITIC PROTOZOA AND METHODS FOR THEIR INDUCTION AND PURIFICATION

Pub. No.:    WO/2018/087644    International Application No.:    PCT/IB2017/056917
Publication Date: Fri May 18 01:59:59 CEST 2018 International Filing Date: Tue Nov 07 00:59:59 CET 2017
IPC: C12N 1/00
A61K 35/68
A61K 39/002
Applicants: CENTRO DE INVESTIGACIÓN Y DE ESTUDIOS AVANZADOS DEL INSTITUTO POLITÉCNICO NACIONAL
Inventors: SIERRA LÓPEZ, Francisco
CARREÑO SÁNCHEZ, Luis Antonio
ROSALES ENCINA, José Luis
Title: IMMUNOGENIC GIANT EXTRACELLULAR VESICLES (VEGS) OF PARASITIC PROTOZOA AND METHODS FOR THEIR INDUCTION AND PURIFICATION
Abstract:
Abstract. The present invention relates to giant secreted (extracellular) vesicles (referred to as VEGs) that are associated with parasitic protozoan species, which have dimensions of 500-400 nm in diameter, and to methods to allow their induction, secretion and purification. These vesicles possess proteolytic activity and are composed of a large amount of proteins; in particular, they were obtained from Entamoeba histolytica trophozoites (referred to as Eh VEG) and from other parasitic protozoa, such as Giardia lamblia and Trypanosoma cruzi. The invention also relates to the use of such vesicles, for example Eh VEGs, to immunize mammals susceptible to infection by Entamoeba histolytica. The present invention also describes effective vaccine immunogenic pharmaceutical compositions comprising the induced and isolated VEGs, for example Eh VEGs, which are useful for the treatment and prevention of amebic hepatic abscess. The present invention represents the first report that demonstrates and/or verifies that E. histolytica secretes extracellular vesicles of large dimensions into the culture medium.