Colecciones nacionales e internacionales de patentes

1. (WO1994008034) NOVEL HIRUDINE VARIANT, PROCESS FOR PRODUCING THE SAME, AND ANTICOAGULANT CONTAINING THE SAME AS ACTIVE INGREDIENT

Pub. No.:    WO/1994/008034    International Application No.:    PCT/JP1993/001384
Publication Date: Fri Apr 15 01:59:59 CEST 1994 International Filing Date: Wed Sep 29 00:59:59 CET 1993
IPC: A61K 38/00
C07K 14/815
Applicants: JAPAN ENERGY CORPORATION

SUKESADA, Akiko

MISAWA, Satoru

MATSUDA, Hitoshi

Inventors: SUKESADA, Akiko

MISAWA, Satoru

MATSUDA, Hitoshi

Title: NOVEL HIRUDINE VARIANT, PROCESS FOR PRODUCING THE SAME, AND ANTICOAGULANT CONTAINING THE SAME AS ACTIVE INGREDIENT
Abstract:
A hirudine variant having the sequence represented by the sequence identification number 1. A DNA coding for the amino acid sequence of the variant, an expression vector, a recombinant colon bacillus, and a process for producing the variant by using the bacillus. An anticoagulant containing the variant as the active ingredient. Because this variant is deficient in the sequence Asp?33-Gly34¿- or Asp?62-Ala63¿- contained in the amino acid sequences of the conventional hirudine and variants thereof, it can inhibit the formation of the succinimide derivative and β-transfer derivative otherwise caused by the above sequence and has a high stability and a high antithrombine activity, thus being useful as an anticoagulant. Furthermore, a hirudine variant having the sequence represented by the sequence identification number 1, wherein the sequence VAl1-VAl2- contained in the amino acid sequence of the conventional hirudine variants has been replaced by the sequence Ile1-Ile2-, has a significantly high antithrombin activity especially suitable for the use as an anticoagulant.