A method for extracting nucleic acids from a biological sample (such as plasma) using nanopores in a porous material. The method induces nucleic acids into nanopores of the porous material to achieve separation and purification of nucleic acids. The method also has the advantage that the bound nucleic acids will not separate from the pores under separation and cleaning conditions, and thus has good application prospects. The material containing nanopores can have added paramagnetic cores (for example, magnetic microspheres of ferroferric oxide) and be applied to a full-automatic nucleic acid extraction workstation to improve nucleic acid extraction efficiency.

This disclosure relates generally to lentiviral vectors useful for the treatment of a disease or condition, for example, Wiskott-Aldrich Syndrome (WAS) or Sickle Cell Disease (SCD).

Compositions and methods for depleting B cells and/or eliminating neutralizing antibodies, particularly for AA V gene therapy administration, are disclosed. Neutralizing alloantibodies (NAbs) to adeno-associated virus (AAV) occur both naturally after exposure to the wildtype circulating virus and following AAV vector mediated gene therapies. When pre-existing, these NAbs preclude eligibility to receive curative AAV gene therapy vectors. Following AAV infusion, the development of NAbs preclude re-administration even with other vector serotypes due to cross-reactivity. Given waning of transgene activity seen in multiple AAV gene therapy trials for hemophilia and the potential need to boost responses with growth in pediatric patients, the ability to re-administer AAV vectors is critical.

The present invention provides a vector engineered virus designed to favor and infect cancers and surrounding cells. This technique mitigates cancer's ability to evade the body's immune system by calling attention to the cells that are cancerous beginning with their earliest stage of development by infecting those cells with a vector engineered virus specifically designed to target tumors in formation. This invention teaches a system and method for identifying, tagging, targeting, and destroying cancer cells while preserving healthy tissue. Developing cancers have two primary traits/biomarkers in common; one being a higher than normal heat signature from elevated metabolic activity; with the second being an acidic or lower than normal pH resulting from the hypoxic micro environment resulting from the depletion of available oxygen. Simply stated, all or most cancers have a heat signature greater than that of normal healthy cells and a pH lower than seen around normal healthy cells.