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Analysis

1 / 29,110
1.WO/2025/260001BIOPROCESSING SYSTEM AND METHODS
WO - 18.12.2025
Int.Class C12M 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
 Appl.No PCT/US2025/033574 Applicant DONALDSON COMPANY, INC. Inventor FISCHER, Vincent J.
A bioprocessing system including a contained bioprocessing environment, a separator assembly, a first retentate flow line, a second retentate flow line, and a retentate system outlet flow line. The separator assembly includes a separator inlet, a retentate outlet, and a permeate outlet. The separator assembly is configured to be fluidly coupled to the contained bioprocessing environment. The first retentate flow line is configured to extend from the retentate outlet to the separator inlet. The second retentate flow line is configured to extend from the retentate outlet to the contained bioprocessing environment. The retentate system outlet flow line is configured to extend from the retentate outlet to a system outlet.
2.WO/2025/259506BIOSENSOR-INTEGRATED MULTI-ORGAN-ON-A-CHIP SYSTEM FOR REAL-TIME AND LONG-TERM MONITORING OF ORGANOID FUNCTION
WO - 18.12.2025
Int.Class C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
 Appl.No PCT/US2025/032363 Applicant WAKE FOREST UNIVERSITY HEALTH SCIENCES Inventor JEON, Seunggyu
The present disclosure describes a multi-organ-on-a-chip system. The system includes a polydimethylsiloxane (PDMS)-based platform, a multi-organoid chamber coupled to the PDMS-based platform, an automated media exchange system coupled to the multi-organoid chamber, and a plurality of sensors coupled to the multi-organoid chamber and the automated media exchange system. The plurality of sensors includes at least one aptamer-based protein sensor and at least one environmental sensor. The system further includes a peristaltic pump in fluid communication with the multi-organoid chamber and the automated media exchange system. The peristaltic pump is configured to circulate a media in a continuous loop through one or more flow lines connecting the multi-organoid chamber, the plurality of sensors, and the automated media exchange system.
3.WO/2025/259746ASSAY METHOD AND DEVICE FOR THE DETECTION OF RARE EVENTS IN CELLS
WO - 18.12.2025
Int.Class G01N 33/50
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
 Appl.No PCT/US2025/033135 Applicant 100XBIO, INC. Inventor PUSTYLNIKOV, Sergei
Described are systems for cell culture analysis and methods for the use thereof, wherein the system comprises a plurality of cell culture chambers and a receptacle for receiving the cell culture chambers, v herein the receptacle comprises one or more receptacle chambers each having a. wail and a. floor. Each cell culture chamber comprises a side wall, an opening on the top of the cell culture chamber, and a semi-permeable membrane on the bottom of the cell culture chamber, and i) wherein the membrane is a small distance from the floor of the receptacle chamber and/or ii) wherein the total surface area of the membrane on the plate is 50% or more of the surface area of the receptacle bottom. The systems and methods allow the detection of rare cell types, rare events, and/or higher cell numbers, including the ability to contact the cells with different analytes for extended times and/or for multiple treatment cycles.
4.WO/2025/258522CELL-CULTURING CONTAINER
WO - 18.12.2025
Int.Class C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
 Appl.No PCT/JP2025/020641 Applicant SUMITOMO RIKO COMPANY LIMITED Inventor IKEGAMI Keiichi
The present invention addresses the problem of simplifying the structure of a cell-culturing container (1) and suppressing the generation of a dead space. A cell-culturing container (1) is provided with a container body (2) having a bag part (20) and a culture chamber (21) which is partitioned inside the bag part (20) and in which a content liquid (A) containing cells and a culture solution for culturing cells is stored. The bag part (20) has: an expansion/contraction section (200) that has expansion/contraction properties and gas permeability over the entire part; and a port section (201) that is contiguous with the expansion/contraction section (200) and has an opening (201c) that is in communication with the culture chamber (21). The volume of the culture chamber (21) is variable in association with the expansion and contraction of the expansion/contraction section (200).
5.WO/2025/259722DEVICES AND METHODS FOR COMPOSITE-LAMINAR FLOW ELEMENTS
WO - 18.12.2025
Int.Class B01L 1/02
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
1Enclosures; Chambers
02Air-pressure chambers; Air-locks therefor
 Appl.No PCT/US2025/033086 Applicant EMBRIENT, INC. Inventor ROTHENBERG, Barry E.
Devices and methods are presented in which a mobile air flow control unit, configured to allow on-demand assembly, controls the atmosphere within a confined space. Preferably, the mobile air flow control unit reduces particle and/or germ count of air within the confined space by producing both a secondary air veil that re-circulates purified air within the confined space and a primary air veil "barrier" across the opening of the confined space.
6.WO/2025/257832ISOLATION OF SVF CELLS FROM LIPOASPIRATE THROUGH ULTRASONIC CAVITATION AND MECHANICAL PROCESSING IN LIQUID BATH
WO - 18.12.2025
Int.Class C12M 1/06
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
04with gas introduction means
06with agitator, e.g. impeller
 Appl.No PCT/IL2025/050509 Applicant CELL-LIPO LTD Inventor SHALTIEL GOLD, Galit
An apparatus for isolation of stromal vascular fraction cells from lipoaspirate includes a tub; a sonotrode attached to an exterior to the tub; and a mixer configured to mix lipoaspirate within the tub. The blades may comprise sets of blades arranged at different heights, wherein each set of blades may be operated independently of the others. The tub, sonotrode, and mixer may be formed as one integral unit that is removably attachable to a motor, wherein, when attached, the motor is configured to rotate the mixer. A method of isolating stromal vascular fraction cells from lipoaspirate includes introducing the lipoaspirate into a tub; introducing fluid into the tub; while mixing the lipoaspirate, applying ultrasonic energy to the interior of the tub from a sonotrode, to thereby induce ultrasonic cavitation within the fluid; and centrifuging the lipoaspirate and fluid to thereby produce a pellet of stromal vascular fraction cells.
7.WO/2025/259286SYSTEMS AND METHODS FOR CONTINUOUS CULTIVATION AND RECOVERY OF COMESTIBLE NON-HUMAN ANIMAL CELLS
WO - 18.12.2025
Int.Class C12M 3/06
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
06with filtration, ultrafiltration, inverse osmosis or dialysis means
 Appl.No PCT/US2024/033952 Applicant UPSIDE FOODS, INC. Inventor BENTON, Charles Knight
The present disclosure relates to systems, apparatuses, and methods for continuous cultivation and recovery of comestible non-human animal cells in a suspension bioreactor. For example, in one or more implementations, the disclosed methods include cultivating non-human animal cells in suspension culture within a bioreactor vessel, removing a measure of suspension culture from the bioreactor vessel while continuing to provide cell culture media thereto, and recovering a first portion of cultivated non-human animal cells from the removed measure of suspension culture while returning a second portion of cultivated non-human animal cells to the bioreactor vessel. Also, in some implementations, the disclosed systems include at least one sterile output module configured to prevent backflow contamination when recovering cultivated cells and removing spent media during continuous cultivation of non-human animal cells according to the disclosed methods.
8.WO/2025/255881STEM CELL CULTURE SYSTEM CAPABLE OF USING MICRO-FLUIDIC CHIP TO CONTROL CONCENTRATION GRADIENT, AND PREPARATION METHOD THEREFOR
WO - 18.12.2025
Int.Class C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
 Appl.No PCT/CN2024/103494 Applicant SHANDONG KEJIN BIOLOGICAL DEVELOPMENT CO., LTD Inventor WANG, Lu
A cell culture system, which solves the technical problems of cell contamination and the impact on the efficiency and quality of cell growth, resulting from a solution with a concentration gradient being prepared in different environments. The specific structure of the system is configured so that the system has M inlets at the upper part, has N outlets at the middle, and has, at the lower part, N stem cell culture chambers respectively in communication with the outlets, wherein P-level branch channels are provided between the inlets and the outlets. A solution with a controllable linear concentration gradient can be generated by means of a micro-fluidic chip, and then flows into a cell culture plate layer . The system aims to simulate a microenvironment in which cells naturally proliferate and differentiate, and also to enhance the efficiency and controllability of cell culture. By means of integrating with microfluidics technology, the system can achieve directional proliferation and differentiation of specific types of stem cells; finally, on the basis of the concentration gradient of a solution, the tendency of cell growth or the impact on cells caused by the same reagent having different concentrations can be explored.
9.WO/2025/259676HOLLOW CHANNEL PRINTING
WO - 18.12.2025
Int.Class B29C 64/209
BPERFORMING OPERATIONS; TRANSPORTING
29WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
CSHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
64Additive manufacturing, i.e. manufacturing of three-dimensional objects by additive deposition, additive agglomeration or additive layering, e.g. by 3D printing, stereolithography or selective laser sintering
20Apparatus for additive manufacturing; Details thereof or accessories therefor
205Means for applying layers
209Heads; Nozzles
 Appl.No PCT/US2025/033010 Applicant THE PENN STATE RESEARCH FOUNDATION Inventor OZBOLAT, Ibrahim Tarik
This document provides bioprinting systems and methods of generating one or more hollow channels in a soft matrix. This document also provides methods of generating one or more hollow channels in the soft matrix that can comprise translating the nozzle of the bioprinting device within the soft matrix. This document also provides methods for generating one or more branched channels within the soft matrix.
10.WO/2025/258669SURFACE STRUCTURE FOR CELL-CULTURING SUBSTRATE, AND CELL-CULTURING CHIP
WO - 18.12.2025
Int.Class C12M 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
 Appl.No PCT/JP2025/021348 Applicant USHIO DENKI KABUSHIKI KAISHA Inventor TAKAHASHI,Seiichiro
This surface structure is for a cell-culturing substrate having a surface on which cells are to be cultured. The surface has, at least in a region where cells make contact, recesses into which biological molecules for adhering the cells to the surface can enter. This surface structure is for a cell-culturing substrate having a surface on which cells are to be cultured. The surface has a plurality of protrusions at least in a region where cells make contact. The plurality of protrusions form, between the protrusions, troughs into which biological molecules for adhering the cells to the surface can enter.
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