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IC:C12M

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1 / 29,208
1.WO/2026/012999METHOD OF HIGH-DENSITY CELL CULTURE AND CELLS THEREOF
WO - 15.01.2026
Int.Class C12N 5/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
 Appl.No PCT/EP2025/069335 Applicant HEMOSTOD SA Inventor DAHAN, Elodie
The present invention provides a method of high-density cell culture comprising at least the following steps: S1: obtain hydrogel microcapsules through membrane emulsification; S2: encapsulate a selected number of cells per each obtained hydrogel microcapsule, and S3: culture the encapsulated cells in a cell culture medium. The invention further refers to Human Pluripotent Stem Cells (hPSCs) obtained through the above-described method, in particular for use in medicine, more in particular for use in a method of in vitro generation of mature megakaryocytes.
2.WO/2026/011199MICROCARRIER FOR CULTIVATING AND/OR EXPANDING CELLS
WO - 15.01.2026
Int.Class C12M 1/12
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
12with sterilisation, filtration, or dialysis means
 Appl.No PCT/AT2025/060273 Applicant TECHNISCHE UNIVERSITÄT WIEN Inventor OVSIANIKOV, Aleksandr
The invention relates to a microcarrier (1) for cultivating and/or expanding cells (Z) in a bioreactor, comprising an at least partly fluid-permeable outer shell (2) and an inner volume (3) for receiving the cells (Z). The microcarrier is characterized in that the outer shell (2) has a geometric shape with a flow resistance which has a minimum in a spatial direction and a fluid permeability which has a minimum in the front region of the outer shell (2), when viewed in said spatial direction.
3.WO/2026/015129LYSIS MODULE WITH CLOSED-LOOP CONTROL MECHANISM
WO - 15.01.2026
Int.Class C12M 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
 Appl.No PCT/US2024/037105 Applicant HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P. Inventor LEONI, Napoleon Jose
A system may include a sonotrode to contact a first portion of a lysing chamber to provide ultrasonic energy to the lysing chamber, a heat spreader to contact a second portion of a lysing chamber to provide thermal energy to the lysing chamber, an infrared sensor to measure a temperature of the lysing chamber, and a controller to compare the temperature of the lysing chamber to a temperature profile and control the sonotrode based on the comparison of the temperature of the lysing chamber to the temperature profile.
4.20260014562DEVICES AND METHODS FOR SEPARATING CELLS OR CELL FRAGMENTS
US - 15.01.2026
Int.Class B01L 3/00
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
 Appl.No 18992400 Applicant Allegheny Singer Research Institute Inventor Joseph M. Ahearn

The present disclosure provides novel devices and methods for separating cells or cell fragments through fine control of a flow rate in the microchannel using a combined force of capillary and gravity without any external pumps. The disclosed devices and methods are unexpectedly effective in capturing various target cells, including rare bioparticles such as B cells, using only a small amount of blood sample.

5.20260015568CELL PORATING AND OPTICALLY DETECTING MICROFLUIDIC DEVICES
US - 15.01.2026
Int.Class C12M 1/42
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
42Apparatus for the treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic wave
 Appl.No 18880135 Applicant Hewlett-Packard Development Company, L.P. Inventor Kenneth John Ward

Examples are directed to a microfluidic device that includes a reservoir to contain a fluid including at least a cell and at least a molecular probe, a cell-poration region fluidically coupled to the reservoir and including a cell-poration mechanism, and an optical-detection region fluidically coupled to the cell-poration region and including an optically transparent window associated with a wall of the optical-detection region. The microfluidic device further includes a fluid ejector fluidically coupled to the optical-detection region.

6.WO/2026/012094SAMPLE REACTION ACCOMMODATION DEVICE AND METHOD
WO - 15.01.2026
Int.Class B01L 3/00
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
 Appl.No PCT/CN2025/102301 Applicant HANGZHOU YUEZHEN BIOTECHNOLOGY CO., LTD Inventor ZHENG, Jianfei
Disclosed in the present invention is a sample reaction accommodation device, comprising: a box body; a pressing cover, which is selectively snap-fitted on the upper portion of the box body; an opening, which penetrates through the pressing cover; and a substrate, on which a sample is provided, the substrate being detachably connected between the box body and the pressing cover, a liquid sealing barrier being provided between the substrate and the pressing cover, and the liquid sealing barrier sealingly abutting against the upper end of the substrate under the pressing action of the pressing cover. The present invention is compatible with substrates of all types (transparent, translucent and opaque), is convenient to operate, saves reaction reagents, effectively eliminates cross contamination between different samples, not only can be used for manual processing procedures, but also is applicable to automated instruments, and separately arranges a sample region and a waste liquid removal region, thereby solving the problem that samples are prone to being punctured during waste liquid removal.
7.WO/2026/013729CLOSED CULTURE VESSEL, CELL CULTURE DEVICE, AND CELL CULTURE METHOD
WO - 15.01.2026
Int.Class C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
 Appl.No PCT/JP2024/024625 Applicant HITACHI, LTD. Inventor KATO Midori
The following configuration is used to provide: a closed cell culture vessel equipped with a mechanism capable of reliably discharging the old culture liquid in a culture vessel; a cell culture apparatus; and a cell culture method.　Provided is a closed culture vessel that has an open culture vessel, a closed vessel for sealing the open culture vessel, a liquid feed port for supplying liquid to at least the open culture vessel, and a liquid discharge port for discharging the liquid in the open culture vessel, each above the closed vessel. The liquid feed port is located above the open surface of the open culture vessel, and the tip of a drainage pipe connected to the liquid discharge port is positioned in the vicinity of the bottom surface of the open culture vessel. Also provided are a cell culture device in which the cell culture vessel can be installed, and a cell culture method.
8.WO/2026/015754SYSTEM AND COMPOSITIONS FOR LASER DISSECTION AND METHODS OF USING THE SAME
WO - 15.01.2026
Int.Class G01N 1/28
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
1Sampling; Preparing specimens for investigation
28Preparing specimens for investigation
 Appl.No PCT/US2025/037196 Applicant THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY Inventor DORKA, Wilhelm Nicolai
The disclosure relates to a system comprising a laser, a membrane, one or a plurality of cells positioned on or adherent to the membrane, and one or plurality of enclosure elements positioned around the one or plurality of cells and membrane, the enclosure elements defining a volume between the enclosure element and the membrane. The disclosure also relates to methods of using the system for microdissection of fixed and living cells on a single-cell level.
9.WO/2026/014359CELL STORAGE CONTAINER AND CELL ACCOMMODATION METHOD
WO - 15.01.2026
Int.Class C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
 Appl.No PCT/JP2025/024057 Applicant JMS CO., LTD. Inventor SHIBUYA Makoto
The present invention provides a cell storage container that facilitates the introduction of cells into a cell accommodation part.　A cell storage container 1 comprises a container body 10 having a cell accommodation part 11 with an internal space for accommodating cells, and a cylindrical cell introduction part 100 connected to the container body 10 for introducing cells into the cell accommodation part 11, wherein the cell accommodation part 11 is formed of a soft member made of a soft material, the ratio of the capacity V2 of an internal space of the cell introduction part 100 to the capacity V1 of the internal space of the cell accommodation part 11 being V2/V1 = 0.43 or less.
10.WO/2026/015154MICROFLUIDIC DEVICE WITH SENSOR
WO - 15.01.2026
Int.Class C12M 3/06
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
06with filtration, ultrafiltration, inverse osmosis or dialysis means
 Appl.No PCT/US2024/037908 Applicant HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P. Inventor SHKOLNIKOV, Viktor
A microfluidic device includes a fluid ejector including a fluid actuator, a microfluidic channel fluidly coupled to the fluid ejector, a sensor positioned along the microfluidic channel, a constriction region positioned between the sensor and the fluid ejector in which an effective cross-sectional area of the microfluidic channel is gradually reduced, and a pair of electrodes positioned in the microfluidic channel between the constriction region and the fluid ejector.
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