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Analysis

1.WO/2008/020739METHOD FOR EXTRACTING α-SPECIFIC TROMBIN-LIKE ENZYME (ANCISTRON-B) FROM AGKISTRODON BLOMHOFFII USSURIENSIS VENOM
WO 21.02.2008
Int.Class A61K 35/58
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
56Materials from animals other than mammals
58Reptiles
Appl.No PCT/MN2006/000001 Applicant WISDOM ASSET COMPANY Inventor VOLKOV Georgiy
The invention relates to producing bioactive substances and can be used for biotechnological processes, in medicine and in the pharmacological industry, in particular to producing medicinal and diagnosis preparations from snake venom. The inventive method consists in carrying out affinity chromatography using a sodium chloride step gradient, wherein, at the first stage, 10mM Tris HCl with a pH of 7.4 is used in the form of a buffer solution and said solution having a sodium chloride concentration of 2M is used in the form of an eluent. The albumin peak exhibiting trombin-like activity is collected, concentrated and, with a view to substitutea buffer, is applied to a column with Sephadex G 25 which is balanced by a 50mM Tris HCl buffer with a pH of 7.4 and a 0.13 M NaCl content.
2.WO/2008/020741METHOD FOR EXTRACTING PHOSPHOLIPASE A2 FROM AGKISTRODON BLOMHOFFII VENOM
WO 21.02.2008
Int.Class A61K 35/58
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
56Materials from animals other than mammals
58Reptiles
Appl.No PCT/MN2006/000003 Applicant WISDOM ASSET COMPANY Inventor VOLKOV Georgiy
The invention relates to producing bioactive substances and can be used for biotechnological production processes, in medicine and in the pharmacological industry, in particular to producing medicinal and diagnosis preparations from snake venom. The inventive method consists in carrying affinity chromatography on a column with Blue Sepharose FF carrier, in diluting a material fraction unconjugated with Blue Sepharose and in applying it to a column filled with a DEAE Sepharose FF anion exchanger, the unconjugated material being washed out from a sorbent, in passing a Tris HCl NaCl-containing buffer with a pH of 8.2 thoughthe column, in eluting phospholipase A2 from the sorbent with a step gradient of NaCl ionic strength. The albumin peak exhibiting phospholipase activity is collected, concentrated and is dried by lyophilising.
3.WO/2008/020743METHOD FOR EXTRACTING A PLATELET AGGREGATION INHIBITOR FROM AGKISTRODON BLOMHOFFII USSURIENSIS VENOM
WO 21.02.2008
Int.Class A61K 35/58
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
56Materials from animals other than mammals
58Reptiles
Appl.No PCT/MN2006/000005 Applicant WISDOM ASSET COMPANY Inventor VOLKOV Georgiy
The invention relates to producing bioactive substances and can be used for biotechnological production processes, in medicine and in the pharmacological industry, in particular to producing medicinal and diagnosis preparations from snake venom. The inventive method consists in carrying affinity chromatography on a column with a Blue Sepharose FF carrier using a Tris HCl with a PH of 7.4 in the form of a buffer solution, in diluting 8 times a material fraction unconjugated with Blue Sepharose in the Tris HCl buffer with a pH of 8.2 and in applying it to a column which is filled with a DEAE Sepharose FF anion exchanger and is pre-balanced by said Tris HCl buffer, in eluting a platelet aggregation inhibitor in the Tris HCl buffer containing NaCl, in collecting and concentrating the protein peak containing the platelet aggregation inhibitor and, with a view to substitute a buffer, in applying said peak to a column with Sephadex G25, which is balanced with the NaCl-containing HCl buffer, and in drying it by lyophilising.
4.WO/2008/020740METHOD FOR EXTRACTING PROTEIN C ACTIVATOR FROM AGKISTRODON BLOMHOFFII USSURIENSIS VENOM
WO 21.02.2008
Int.Class A61K 35/58
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
56Materials from animals other than mammals
58Reptiles
Appl.No PCT/MN2006/000002 Applicant WISDOM ASSET COMPANY Inventor VOLKOV Georgiy
The invention relates to producing bioactive substances and can be used for biotechnological production processes, in medicine and in the pharmacological industry, in particular to producing medicinal and diagnosis preparations from snake venom.The inventive method consists in carrying affinity chromatography on a column with a Blue Sepharose FF carrier using a buffer solution and in applying it to a column filled with a DEAE Sepharose FF anion exchanger, in washing out unconjugated material from a sorbent by an application buffer, in eluting a protein C activator from the sorbent together with a fibrinolytic enzyme, in collecting and diluting the protein peak containing said enzymes, in applying said peak to a column with a CM Sepharose cation exchanger, in dividing it with a NaCl (0-0.2M) linear gradient in a corresponding buffer, in collecting the protein peak containing protein C activator and in drying it by lyophilising.
5.WO/2008/020742METHOD FOR EXTRACTING FIBRINOGENIC/FIBRINOLYTIC PROTEIN FROM AGKISTRODON BLOMHOFFII USSURIENSIS VENOM
WO 21.02.2008
Int.Class A61K 35/58
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
56Materials from animals other than mammals
58Reptiles
Appl.No PCT/MN2006/000004 Applicant WISDOM ASSET COMPANY Inventor VOLKOV Georgiy
The invention relates to producing bioactive substances and can be used for biotechnological production processes, in medicine and in the pharmacological industry, in particular to producing medicinal and diagnosis preparations from snake venom. The inventive method consists in carrying affinity chromatography on a column with a Blue Sepharose FF carrier using a buffer solution and in applying it to a column filled with a DEAE Sepharose FF anion exchanger, in washing out unconjugated material from a sorbent by an application buffer, in eluting a fibrinogenic/fibrinolytic protein from the sorbent, in collecting and diluting the protein peak containing said proteins, in applying said peak to a column with a CM Sepharose cation exchanger, in dividing it with a NaCl (0-0.2M) linear gradient in a corresponding buffer, in collecting the protein peak containing the fibrinogenic/fibrinolytic protein and in drying it by lyophilising.
6.WO/2008/020744METHOD FOR EXTRACTING THE NERVE TISSUE GROWTH FACTOR FROM V.LEBETINA LINNAEUS VENOM
WO 21.02.2008
Int.Class A61K 35/58
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
35Medicinal preparations containing materials or reaction products thereof with undetermined constitution
56Materials from animals other than mammals
58Reptiles
Appl.No PCT/MN2006/000006 Applicant WISDOM ASSET COMPANY Inventor VOLKOV, Georgiy
The invention relates to producing bioactive substances and can be used for biotechnological production processes, in medicine and in the pharmacological industry, in particular to producing medicinal and diagnosis preparations from snake venom. The inventive method consists in carrying affinity chromatography prior to a fractional chromatography in two steps on a column with a Source 15S carrier by using, at a first step, a sodium carbonate solution with a pH of 6.5-6.6 in the form of a 0.19-0.21 M buffer solution and said solution with a concentration of 0.31-0.33M in the form of an eluent, in diluting 4-5 times the thus obtained eleuent with distilled water and in re-chromatographing on a column with the same carrier but less by 10 times in volume by using a sodium carbonate solution with a pH of 5.0-5.2 in the form of a 0.045-0.55 M buffer solution and the same solution with a concentration of 0.95-1.05M and a pH of 6.4-6.6 in the form of an eleuent, and in carrying out fractional chromatography of the thus obtained eluent on A Superdex 75 with a 0.19-0.21 ammonium bicarbonate solution with a pH of 6.4-6.6.