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Analysis

1.20210130916PROBE FOR DETECTING HEPATITIS B VIRUS AND USE THEREOF
US 06.05.2021
Int.Class C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
Appl.No 17089432 Applicant THE CATHOLIC UNIVERSITY OF KOREA INDUSTRY-ACADEMIC COOPERATION FOUNDATION Inventor Jeong Won JANG

A probe for detecting hepatitis B virus and a method for detecting an insertion site of hepatitis B virus at high efficiency based on the analysis method of next-generation sequencing using the probe is disclosed. A probe can be provided that is capable of confirming the insertion site of HBV in the human genome with a possibility of developing into liver cancer. In addition, by applying the probe to the analysis method of next-generation sequencing, HBV insertion sites in the human genome can be analyzed at low cost and high efficiency.

2.WO/2021/083429CODON-OPTIMIZED GLUCOSE OXIDASE GENE AND APPLICATION THEREOF
WO 06.05.2021
Int.Class C12N 15/53
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
53Oxidoreductases (1)
Appl.No PCT/CN2020/140668 Applicant BIOLOGY INSTITUTE OF SHANDONG ACADEMY OF SCIENCES Inventor GONG, Weili
The invention provides a codon-optimized glucose oxidase gene. The gene is derived from Aspergillus niger An76, and the sequence thereof is as shown in SEQ ID No. 2. The initial glucose oxidase gene cannot be successfully expressed in Pichia pastoris, but can be successfully expressed in Pichia pastoris following sequence optimization. In addition, a purified target protein can be obtained by means of separation and purification, and the recombinant glucose oxidase may be used in glucose biosensors.
3.WO/2021/084419METHOD FOR MAPPING ROLLING CIRCLE AMPLIFICATION PRODUCTS
WO 06.05.2021
Int.Class C12Q 1/6816
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
Appl.No PCT/IB2020/060062 Applicant PIXELGEN TECHNOLOGIES AB Inventor FREDRIKSSON, Simon
Provided herein is a method for mapping rolling circle amplification (RCA) products that contain unique identifier sequences. The method generally involves (a) producing a complex comprising population of grid oligonucleotide molecules and a population of RCA products that each have a unique RCA product identifier sequence, wherein the grid oligonucleotides are hybridized directly or indirectly via a splint to complementary sites in the RCA products; (b) extending the grid oligonucleotide molecules that are hybridized to two RCA products to add the complements of the unique RCA product identifier sequences from the two RCA products to the grid oligonucleotide molecules; (c) sequencing the extended grid oligonucleotides; (d) analyzing the sequences to identify which pairs of unique RCA product identifier sequence complements have been added onto the grid oligonucleotides; and (e) making one or more physical maps of the immobilized RCA products using the pairs of sequences identified in (d).
4.WO/2021/084539A METHOD FOR DETERMINING BACTERIAL SUSCEPTIBILITY TO ANTIBIOTICS
WO 06.05.2021
Int.Class C12Q 1/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
02involving viable microorganisms
04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
Appl.No PCT/IL2020/051129 Applicant THE ISRAEL INSTITUTE OF BIOLOGICAL RESEARCH (IIBR) Inventor ALONI-GRINSTEIN, Ronit
The invention concerns methods for determining antimicrobial susceptibility of a microorganism (e.g. bacteria, fungi), comprising incubating a biological or environmental sample on a solid growth substrate containing at least one antimicrobial agent at varying concentrations for a relatively short period of time; recovering the microorganism growing on the solid growth substrate surface; and determining the microorganism quantity using a quantitative method, e.g. qPCR, and comparing the quantity to that of a sample grown on a solid substrate without antimicrobial agent. The method is particularly useful for Yersinia pestis, Bacillus anthracis and Francisella tularensis.
5.WO/2021/085034METHOD FOR SELECTING PLURIPOTENT STEM CELLS, METHOD FOR PREDICTING RESULT OF DIFFERENTIATION INDUCTION, AND METHOD FOR PRODUCING CELL PRODUCT
WO 06.05.2021
Int.Class G01N 21/17
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
21Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
17Systems in which incident light is modified in accordance with the properties of the material investigated
Appl.No PCT/JP2020/037591 Applicant FUJIFILM CORPORATION Inventor MURAKAMI, Yuta
A method for selecting pluripotent stem cells, said method comprising: from a hologram obtained by imaging an aggregate of pluripotent stem cells, generating a phase difference image of the aggregate; on the basis of the phase difference image, deriving an indicator value, which is determined depending on the relationship between the area of the aggregate and the outline length thereof and which indicates the complexity or simplicity of the outline; and then selecting the pluripotent cells on the basis of the indicator value.
6.WO/2021/085649CELL EVALUATION METHOD, CELL EVALUATION SYSTEM AND PROGRAM
WO 06.05.2021
Int.Class C12M 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
Appl.No PCT/JP2020/041084 Applicant PUBLIC UNIVERSITY CORPORATION YOKOHAMA CITY UNIVERSITY Inventor TANIGUCHI Hideki
This cell evaluation method comprises: in a culture stage involving the induction of cell differentiation during a process for inducing the cell differentiation, acquiring a first evaluation index relative to cells to be compared and a first index calculated with the use of the first evaluation index; calculating a second index relative to cells to be evaluated, said cells to be evaluated being different from the cells to be compared, on the basis of the first evaluation index; and then comparing the first index with the second index to thereby evaluate the differentiation of the cells to be evaluated.
7.WO/2021/086954METHODS AND DEVICES USING CARTRIDGES FOR SEQUENCING
WO 06.05.2021
Int.Class C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction assay
Appl.No PCT/US2020/057722 Applicant QUANTUM-SI INCORPORATED Inventor ROTHBERG, Jonathan M.
Methods and devices for enriching and analyzing target molecules from a sample are provided herein. In some embodiments, methods and devices using cartridges involve enrichment of target molecules (e.g., using SCODA) and detection and analysis using sequencing. The method may employ lysing of cells, fragmentation of target in the sample preparation module and enriching the target in the module and moving the target to the sequencing module.
8.WO/2021/087044METHODS FOR PREDICTING RESPONSIVENESS OF LYMPHOMA TO DRUG AND METHODS FOR TREATING LYMPHOMA
WO 06.05.2021
Int.Class C12Q 1/6886
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6883for diseases caused by alterations of genetic material
6886for cancer
Appl.No PCT/US2020/057860 Applicant CELGENE CORPORATION Inventor TOWFIC, Fadi, George
Provided herein are methods of predicting the responsiveness of a lymphoma patient to a cancer treatment comprising clustering patients into subgroups of patients using gene expression levels. Also provided herein are methods of treating a lymphoma patient based on predicting the responsiveness of the lymphoma patient to a cancer treatment.
9.WO/2021/087164METHODS FOR DETECTING OLIGONUCLEOTIDES
WO 06.05.2021
Int.Class C12N 9/22
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
16acting on ester bonds (3.1)
22Ribonucleases
Appl.No PCT/US2020/058044 Applicant SHIRE HUMAN GENETIC THERAPIES, INC. Inventor SHEN, Yinghua
The present disclosure describes methods for detecting oligonucleotide presence and/or quantity in a sample.
10.WO/2021/087344THERANOSTICS FOR HYPERTENSION INDUCED MYOCARDIAL MICROBLEEDS
WO 06.05.2021
Int.Class C07K 14/705
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
705Receptors; Cell surface antigens; Cell surface determinants
Appl.No PCT/US2020/058335 Applicant CEDARS-SINAI MEDICAL CENTER Inventor DHARMAKUMAR, Rohan
The invention relates to methods of cardiovascular imaging and/or measurement of blood markers for detecting, diagnosing and/or prognosing cardiac or myocardial microbleeds, especially in subject with hypertension and cardiovascular diseases. Treatment methods are also provided for subjects identified, diagnosed, prognosed, or detected with cardiac or myocardial microbleeds. In some embodiments, the subject has hypertension-induced microbleeds, but has not had a myocardial infarction or reperfusion therapy.