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Analysis

1.20250206770CAP COMPOUNDS AND RNAS COMPRISING THE SAME
US 26.06.2025
Int.Class C07H 21/02
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
21Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
02with ribosyl as saccharide radical
Appl.No 18990530 Applicant Helix Nanotechnologies Inc Inventor Nikhil Dhar

Disclosed herein are 5′ cap structures comprising a 2′-O-acetyl ribose. Also provided herein are polyribonucleotides comprising a 5′ cap structure disclosed herein and compositions comprising the same as well as methods of making and using the same.

2.20250207157METHODS OF PRODUCING HYDROXYTYROSOL
US 26.06.2025
Int.Class C12P 7/22
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
02containing a hydroxy group
22aromatic
Appl.No 18791545 Applicant Conagen Inc. Inventor Rui Zhou

Provided herein is a method of producing hydroxytyrosol. The method includes: culturing a recombinant host microorganism in a medium, wherein: the host microorganism has been transformed with: a first polynucleotide sequence encoding a decarboxylase enzyme capable of decarboxylating 4 hydroxyphenylpyruvaic acid (HPP) to produce 4 hydroxyphenylacetaldehyde, and a second polynucleotide sequence encoding a hydroxylase enzyme, wherein the hydroxylase has an amino acid sequence having at least 90% identity to the amino acid sequence as set forth in SEQ ID NO: 2, and the medium comprises glucose; and collecting product hydroxytyrosol from one or both of the host microorganism and the culture medium.

3.20250207165DOUBLE STRANDED CIRCULAR DNA VECTOR, METHOD FOR PRODUCING LINEAR COVALENTLY CLOSED DNA, AND FUSION POLYPEPTIDE CONTAINING PROTELOMERASE AND ENDONUCLEASE
US 26.06.2025
Int.Class C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Appl.No 19076287 Applicant KANEKA CORPORATION Inventor Mitsuki Shigeta

Provided is a double-stranded circular DNA vector, including a protelomerase gene sequence encoding a protelomerase, an endonuclease gene sequence encoding an endonuclease, a pair of protelomerase recognition sequences which are recognized by said protelomerase for cleaving said vector, at least one endonuclease recognition sequence which is recognized by said endonuclease for cleaving said vector, and a nucleic acid sequence of interest. The protelomerase gene sequence, the endonuclease gene sequence, and the endonuclease recognition sequence are placed in the same region between the pair of protelomerase recognition sequences, and the nucleic acid sequence of interest is placed in the other region between said pair of protelomerase recognition sequences in said double-stranded circular DNA vector. The protelomerase gene sequence and the endonuclease gene sequence are placed under the control of a promoter which is capable of regulating the expression.

4.WO/2025/130837PHA EXTRACTION METHOD FOR ADJUSTING CHROMATICITY OF PHA MATERIAL, PRODUCT THEREFROM, AND USE AND PREPARATION METHODS THEREFOR
WO 26.06.2025
Int.Class C08G 63/89
CCHEMISTRY; METALLURGY
08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
GMACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
63Macromolecular compounds obtained by reactions forming a carboxylic ester link in the main chain of the macromolecule
88Post-polymerisation treatment
89Recovery of the polymer
Appl.No PCT/CN2024/139787 Applicant BEIJING PHABUILDER BIOTECHNOLOGY CO., LTD. Inventor LIU, Xingtian
The present invention relates to the technical field of new materials, and in particular to a PHA extraction method for adjusting the chromaticity of a PHA material, a product therefrom, and a use thereof. The extraction method comprises: carrying out solid-liquid separation on a halophilic bacteria fermentation liquid to obtain a concentrated bacterial liquid; and adjusting the pH of the concentrated bacterial liquid to be acidic, adding a complex enzyme formulation I to the concentrated bacterial liquid, enabling the average particle size of the resulting emulsion to be 1-10 μm under a mechanical action, then adjusting the pH to be alkaline, adding a complex enzyme formulation II to the emulsion, and continuing the mechanical action to obtain a wall-disrupted liquid. The aqueous phase extraction method of the present invention has mild conditions and is suitable for large-scale continuous production. By means of the cooperation of the special enzyme formulations and special equipment, various impurities causing yellowing are reduced, so that the chromaticity of PHA pellets obtained by further processing is low. Moreover, during thermoplastic processing, the decrease of the molecular weight can be effectively inhibited, the increase of MFR is inhibited, the toughness of various formed bodies is improved, and the risk of dissolution in a solvent environment in subsequent applications is small.
5.WO/2025/133162RECOMBINANT PROTEIN EXPRESSION
WO 26.06.2025
Int.Class C12N 15/63
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
Appl.No PCT/EP2024/087965 Applicant NOVOZYMES A/S Inventor OLSEN, Carsten, Lillelund
The present invention relates to inducible promoters, and to methods of protein production, wherein recombinant cells are cultivated in a cultivation medium comprising acetate. The invention further relates to formulations and compositions obtained with the method of the invention.
6.WO/2025/133402METHOD FOR PRODUCING 2,5-FURANDICARBOXYLIC ACID
WO 26.06.2025
Int.Class C12P 17/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
17Preparation of heterocyclic carbon compounds with only O, N, S, Se, or Te as ring hetero atoms
02Oxygen as only ring hetero atoms
04containing a five-membered hetero ring, e.g. griseofulvin
Appl.No PCT/EP2024/088407 Applicant ANNIKKI GMBH Inventor STAUNIG, Nicole
The present invention relates to a method for preparing 2,5-furandicarboxylic acid by oxidizing 5-formyl-2-furancarboxylic acid, which is present in an aqueous solution, by treatment with an NAD(P)+dependent oxidoreductase in vitro to 2,5-furandicarboxylic acid, wherein the NAD(P)H formed during the oxidation is enzymatically oxidized back to NAD(P)+ by means of an NAD(P)H oxidase, after which the enzymes are removed.
7.20250207155PARTICLE-BASED BIOSEPARATION ASSAYS
US 26.06.2025
Int.Class C12P 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
1Preparation of compounds or compositions, not provided for in groups C12P3/-C12P39/121; General processes for the preparation of compounds or compositions by using microorganisms or enzymes
Appl.No 18395124 Applicant Seer, Inc. Inventor Robert S LANGER

Apparatus, systems and methods for separation of biomolecules in a biofluid. Separation methods include nanoparticles-based separation (e.g., formation of biomolecule coronas on nanoparticles), serial enrichment, electrophoretic particle separation, chromatography, affinity tag, isocratic elution, gradient and isocratic elution, cleave particle coating, or any combination thereof.

8.20250207160CELL-FREE PRODUCTION OF GERANYL PYROPHOSPHATE FROM GLYCEROL IN A CELL-FREE MANUFACTURING SYSTEM
US 26.06.2025
Int.Class C12P 9/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
9Preparation of organic compounds containing a metal or atom other than H, N, C, O, S, or halogen
Appl.No 18677251 Applicant DEBUT BIOTECHNOLOGY, INC. Inventor Joshua Britton

Geranyl pyrophosphate (GPP) is a key intermediate molecule in the bioproduction of thousands of natural products. Currently, natural products are either cultivated from plants, synthesized via complex chemical synthesis strategies, or through cell-based factories also known as biofoundries. However, in order to replicate the process in a cell free environment, numerous enzymes and cofactors must be utilized making this approach costly and unviable. In order to make this process viable, a new approach was needed that uses fewer enzymes and cofactors. As described herein, the present invention demonstrates that it is possible to create GPP from glycerol through a short and concise biosynthetic pathway outside of the cell.

9.20250207161L-AMINO ACID-PRODUCING MICROORGANISM HAVING WEAKENED CARBAMOYL PHOSPHATE SYNTHASE ACTIVITY, AND L-AMINO ACID PRODUCTION METHOD USING SAME
US 26.06.2025
Int.Class C12P 13/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13Preparation of nitrogen-containing organic compounds
04Alpha- or beta-amino acids
10Citrulline; Arginine; Ornithine
Appl.No 18847615 Applicant CJ CHEILJEDANG CORPORATION Inventor Jinsub PARK

Provided are a microorganism producing L-amino acids, in which activity of carbamoyl phosphate synthetase is weakened, and a method of producing L-amino acids using the same.

10.20250204562MASKING AGENT
US 26.06.2025
Int.Class A23L 27/00
AHUMAN NECESSITIES
23FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; PREPARATION, TREATMENT OR PRESERVATION THEREOF
LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
27Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
Appl.No 18850235 Applicant FUJI OIL HOLDINGS INC. Inventor Misato BABA

Provided is a masking agent for an off-flavor of plant-based protein ingredient, the masking agent containing phenylacetaldehyde and/or 4-hydroxyphenylacetaldehyde.