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Analysis

1.WO/2021/087354HIGH-THROUGHPUT CULTURE OF IPSC-DERIVED ALVEOLAR CELLS
WO 06.05.2021
Int.Class C12N 5/071
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
Appl.No PCT/US2020/058350 Applicant THE GENERAL HOSPITAL CORPORATION Inventor OTT, Harald C.
Provided herein are floating hydrogel droplet culture methods that enable scaling of stem cell derived alveolar epithelial cell (AEC) expansion to numbers compatible with large animal or human whole lung engineering, as well as molds for generating the droplets and methods of use thereof.
2.20210130774HUMAN PLURIPOTENT STEM CELL DERIVED NEURODEGENERATIVE DISEASE MODELS ON A MICROFLUIDIC CHIP
US 06.05.2021
Int.Class C12N 5/079
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
07Animal cells or tissues
071Vertebrate cells or tissues, e.g. human cells or tissues
079Neural cells
Appl.No 17041672 Applicant CEDARS-SINAI MEDICAL CENTER Inventor Samuel SANCES

Described herein is a microphysiological system for models of disease. Specifically, induced pluripotent stem cells (iPSCs) and iPSC-derived cells, including those obtained from disease patients, are seeded onto microfluidic “chip” devices to study cellular development and disease pathogenesis. Herein, neurodegenerative disease modeling, including Parkinson's Disease (PD) is shown to reproduce key PD pathology in a vascularized human model that contains neurons relating to PD pathology. Such compositions and methods are used for research for PD biomarkers, patient screening for PD risk assessment, and therapeutic discovery and testing. A panel of biomarkers are generated through analysis of living PD-chips by neural activity, whole transcriptomic, proteomic, and metabolomic analysis, and functional enzyme tests of media and tissue. Introducing therapeutics through a vasculature channel, coupled with blood brain barrier penetration studies can be assessed for efficacy in the human neural cells present in the PD-Chip.

3.20210130761FIXED BED BIOREACTOR AND METHODS OF USING THE SAME
US 06.05.2021
Int.Class C12M 1/12
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
12with sterilisation, filtration, or dialysis means
Appl.No 17039035 Applicant CORNING INCORPORATED Inventor Ann MeeJin Ferrie

A fixed-bed bioreactor system is provided that includes a vessel with a media inlet, a media outlet, and an interior cavity disposed between and in fluid communication with the media inlet and media outlet. The vessel further includes a cell culture substrate disposed in the interior cavity between the media inlet and the media outlet in a packed-bed configuration, the cell culture substrate including a plurality of porous disks in a stacked arrangement. The interior cavity includes a cell culture section and a spacer section, the cell culture substrate defining the cell culture section and the spacer section being disposed between the cell culture section and the media outlet, and each of the plurality of porous disks has a surface configured to culture cells thereon.

4.WO/2021/087311AGITATION PLATFORM FOR MAINTAINING HOMOGENEITY OF SOLUTIONS
WO 06.05.2021
Int.Class B01F 11/00
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
FMIXING, e.g. DISSOLVING, EMULSIFYING, DISPERSING
11Mixers with shaking, oscillating, or vibrating mechanisms
Appl.No PCT/US2020/058290 Applicant BRAMMER BIO MA LLC Inventor TAN, Yih, Horng
According to embodiments of the present disclosure, apparatuses, systems, and methods are provided that enable the maintenance of homogeneity of biomaterials during aseptic fill-finish. In various embodiments, an apparatus (600) for aseptic fill-finish provided herein includes an agitation device (601). The apparatus further includes a platform (609) coupled to the agitation device. The platform is rotatable about a first axis. The apparatus further includes at least one shaft (603a, 603b) extending from the platform. The shaft is adjustable in length. The apparatus further includes at least one arm (607a, 607b) extending from the shaft. The at least one arm has at least one fixation mechanism (608). The at least one fixation mechanism is configured to receive a container having a sealed compartment containing a biomaterial and maintain the container in a substantially vertical orientation. The platform is configured to engage at least a portion of the container.
5.20210129151LARGE MICROFLUIDIC BIOREACTOR AND MANUFACTURING METHOD THEREOF
US 06.05.2021
Int.Class B01L 3/00
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
Appl.No 17130531 Applicant Shekhar Bhansali Inventor Shekhar Bhansali

Large bioreactors based on microfluidic technology, and methods of manufacturing the same, are provided, The big microbioreactor can include a chip or substrate having the microfluidic channels thereon, and the chip can be manufactured by forming a master mold, forming a male mold from a photopolymer plate using replica molding with the Fmold, and transferring features of the male to a polymer material.

6.WO/2021/084943CULTURE OPERATION DEVICE
WO 06.05.2021
Int.Class C12M 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
Appl.No PCT/JP2020/034669 Applicant ROHTO PHARMACEUTICAL CO., LTD. Inventor SUGIMOTO, Shohei
A culture operation device according to the present invention is for performing an operation associated with culturing of a subject to be cultured, and includes a first dispenser head and a second dispenser head. The first dispenser head is capable of sucking a liquid from a vessel and is also capable of discharging the liquid to another vessel. The second dispenser head is capable of sucking a liquid from a vessel and is also capable of discharging the liquid to another vessel. Also, the retainable liquid capacity of the second dispenser head is greater than that of the first dispenser head.
7.WO/2021/084736CELL CULTURE METHOD AND CELL CULTURE DEVICE
WO 06.05.2021
Int.Class C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
Appl.No PCT/JP2019/043035 Applicant SHIMADZU CORPORATION Inventor MIYAKE, Chikara
This cell culture method comprises: a step in which an environment gas (101) for forming the atmosphere of a cell culture environment is supplied to a first culture chamber (10a), thereby causing a liquid (91) within the first culture chamber to move to a second culture chamber (10b) via a first channel (20a); and a step in which prepared environment gas is supplied to the first culture chamber, replacing the environment gas that was used to move the liquid.
8.WO/2021/084737CELL CULTURE DEVICE
WO 06.05.2021
Int.Class C12M 1/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
04with gas introduction means
Appl.No PCT/JP2019/043036 Applicant SHIMADZU CORPORATION Inventor MIYAKE, Chikara
This cell culture apparatus (100) comprises: a reading unit (137) that reads identification information (1a) attached to a cell culture device (1); and a control unit (140) that performs control regarding the movement of a culture chamber (10) due to a liquid feed gas (101) on the basis of the identification information read by the reading unit.
9.WO/2021/085637CELL CULTURE METHOD, METHOD FOR PRODUCING CULTURE SOLUTION, CULTURE SOLUTION AND CULTURE DEVICE
WO 06.05.2021
Int.Class C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
Appl.No PCT/JP2020/040961 Applicant CANON KABUSHIKI KAISHA Inventor KUBOTA Masahiko
The purpose of the present invention is to efficiently produce three-dimensional cells in a large amount. For this purpose, cells 603 are cultured using a culture solution 602 containing ultrafine bubbles 11 that are generated by heating a heating element 10 and thus causing film boiling at the interface of a liquid W and the heating element.
10.WO/2021/087227METHOD AND SYSTEM FOR CELL OPERATIONS USING A BLOCKCHAIN DATA NETWORK
WO 06.05.2021
Int.Class C12M 1/24
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
24tube or bottle type
Appl.No PCT/US2020/058153 Applicant CANNON, Howard Inventor CANNON, Howard
A method and system for performing operations on cells on an instrument comprises providing a peer to peer network of instruments for performing operations on cells, maintaining a distributed database in at least a plurality of the instruments in the peer to peer network, storing at least one protocol in the database for use by at least one instrument in the peer to peer network for performing operations on cells and storing results from the performance of the operations on cells by the at least one instrument in the distributed database. The results stored in the distributed database is then usable to later authenticate the results from the at least one instrument.