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1.WO/2022/105640NUCLEIC ACID SEQUENCING METHOD
WO 27.05.2022
Int.Class C12Q 1/6869
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6869Methods for sequencing
Appl.No PCT/CN2021/129461 Applicant TSINGHUA UNIVERSITY Inventor BAI, Jingwei
The present invention provides a nucleic acid sequencing method, comprising: providing a polymerase linked to dNTP by means of a cleavable group and capable of emitting an optical signal; making the polymerase in contact with 3' end of a primer of a nucleic acid template-primer complex to be measured, complementarily pairing dNTP on the polymerase with a base group on a nucleic acid sequence to be measured, and adding the 3' end of the primer into a polymerase enzymatic reaction; and detecting the optical signal emitted by the polymerase. Subsequently, dNTP and the polymerase are broken, and the new template-primer complex after the leaving of the polymerase may enter the next sequencing cycle.
2.WO/2022/099938METHOD FOR GENERATING SINGLE-STRANDED CIRCULAR DNA ON BASIS OF LOCK-TYPE PROBE TECHNIQUE, AND USE THEREOF
WO 19.05.2022
Int.Class C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Appl.No PCT/CN2021/074641 Applicant TIANJIN UNIVERSITY Inventor QI, Hao
Provided are a method for generating single-stranded circular DNA on the basis of a lock-type probe technique, and the use thereof. The method comprises designing a lock-type probe and a blocker probe for a single-stranded target gene, then using a DNA ligase for connection, and adding an exonuclease after connection so as to remove the unconnected lock-type probe, thereby obtaining single-stranded circular DNA. The method can be used for detecting single-site variation.
3.WO/2022/104367CELL-FREE EXPRESSION OF ANTIBODIES, ANTIGEN-BINDING FRAGMENTS THEREOF, AND ANTIBODY DERIVATIVES
WO 19.05.2022
Int.Class C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Appl.No PCT/US2021/072371 Applicant NORTHWESTERN UNIVERSITY Inventor VOGELI, Bastian
The present technology relates to cell-free systems, methods, and kits for expressing proteins in vitro and evaluating the expressed proteins. In particular, the technology relates to cell-free systems, methods, and kits for expressing antibodies, antigen-binding fragment thereof, and antibody derivatives in vitro and evaluating the expressed antibodies, antigen-binding fragment thereof, and antibody derivatives.
4.WO/2022/103499SPATIAL CONTROL OF POLYNUCLEOTIDE SYNTHESIS BY STRAND CAPPING
WO 19.05.2022
Int.Class C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Appl.No PCT/US2021/052350 Applicant MICROSOFT TECHNOLOGY LICENSING, LLC Inventor NGUYEN, Bichlien Hoang
Enzymatic polynucleotide synthesis with a template-independent polymerase is used to create multiple polynucleotides having different, arbitrary sequences on the surface of an array. The array provides a spatially-addressable substrate for solid-phase synthesis. Blocking groups are attached to the 3' ends of polynucleotides on the array. Prior to polynucleotide extension, the blocking groups are removed at a selected location on the array. In an implementation, the blocking groups are acyl groups removed with a negative voltage created at an electrode. The array is then incubated with the polymerase and a single species of nucleotide. Nucleotides are incorporated onto the 3' ends of the polynucleotides without blocking groups. Washing removes the polymerase and free nucleotides. To create polynucleotides with different sequences at different locations on the array, the location where the blocking groups are removed and the species of nucleotide may be changed during repeated cycles of synthesis.
5.20220145346COMPOSITIONS AND METHODS FOR TEMPLATE-FREE GEOMETRIC ENZYMATIC NUCLEIC ACID SYNTHESIS
US 12.05.2022
Int.Class C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Appl.No 17422967 Applicant CAMENA BIOSCIENCE LIMITED Inventor Derek L. STEMPLE

Disclosed are compositions and methods for template-free nucleic acid synthesis using N-mers and/or anchor primers that comprise at least one XNA or a combination of RNA and DNA.

6.20220145345SPATIAL CONTROL OF POLYNUCLEOTIDE SYNTHESIS BY STRAND CAPPING
US 12.05.2022
Int.Class C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Appl.No 17095650 Applicant MICROSOFT TECHNOLOGY LICENSING, LLC Inventor Bichlien Hoang NGUYEN

Enzymatic polynucleotide synthesis with a template-independent polymerase is used to create multiple polynucleotides having different, arbitrary sequences on the surface of an array. The array provides a spatially-addressable substrate for solid-phase synthesis. Blocking groups are attached to the 3′ ends of polynucleotides on the array. Prior to polynucleotide extension, the blocking groups are removed at a selected location on the array. In an implementation, the blocking groups are acyl groups removed with a negative voltage created at an electrode. The array is then incubated with the polymerase and a single species of nucleotide. Nucleotides are incorporated onto the 3′ ends of the polynucleotides without blocking groups. Washing removes the polymerase and free nucleotides. To create polynucleotides with different sequences at different locations on the array, the location where the blocking groups are removed and the species of nucleotide may be changed during repeated cycles of synthesis.

7.WO/2022/094863METHOD FOR DETECTING RNA STRUCTURE AT WHOLE TRANSCRIPTOME LEVEL AND USE THEREOF
WO 12.05.2022
Int.Class C12N 15/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
Appl.No PCT/CN2020/126766 Applicant TSINGHUA UNIVERSITY Inventor ZHANG, Qiangfeng
Provided in the present invention are a method for detecting an RNA structure and the use thereof. According to the present invention, the step of removing the background of reverse transcription termination signals is included in the method for detecting an RNA structure, and false positive signals in a structural score calculation are reduced, and therefore the accuracy of the detection method is improved.
8.WO/2022/099010HAIRPIN OLIGONUCLEOTIDES AND USES THEREOF
WO 12.05.2022
Int.Class C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Appl.No PCT/US2021/058258 Applicant THE UNIVERSITY OF CHICAGO Inventor PAN, Tao
In aspects, the invention provides a hairpin oligonucleotide comprising a 3 '-terminal nucleotide, wherein the sugar component of the 3 '-terminal nucleotide comprises a 2'- hydroxyl and a 3 '-phosphate. In aspects, the invention provides a hairpin oligonucleotide comprising a 3 '-terminal nucleotide wherein the sugar position of the 3 '-terminal nucleotide comprises a 2', 3 '-dialdehyde oxidation product of a sugar. In aspects, the invention provides use of a hairpin oligonucleotide in developing a biomarker. In aspects, the invention provides a solid support comprising a ligand moiety and a hairpin oligonucleotide, wherein the oligonucleotide is immobilized on the solid support through binding of the affinity moiety of the hairpin oligonucleotide to the ligand moiety of the solid support. In aspects, the invention also provides a method of preparing an RNA sequence library comprising: (a) ligating an RNA sequence to a hairpin oligonucleotide to form a construct, (b) reverse-transcribing the RNA sequence as a cDNA sequence, and (c) amplifying the cDNA sequence using PCR.
9.20220145347HIGH-EFFICIENCY RECONSTITUTION OF RNA MOLECULES
US 12.05.2022
Int.Class C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Appl.No 17486488 Applicant Salk Institute for Biological Studies Inventor Lukas Christoph Bachmann

Provided herein are synthetic RNA molecules for reconstitution of RNA molecules, including compositions and methods of using these molecules. For example, such molecules can be used to deliver a protein coding sequence over two or more viral vectors (such as AAVs), resulting in reconstitution of the full-length protein in a cell. Such methods can be used to deliver a therapeutic protein, for example to treat a genetic disease or cancer.

10.WO/2022/090323ENZYMATIC SYNTHESIS OF POLYNUCLEOTIDE PROBES
WO 05.05.2022
Int.Class C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Appl.No PCT/EP2021/079847 Applicant DNA SCRIPT Inventor GODRON, Xavier
The present invention is directed to methods and kits for enzymatic synthesis of labeled polynucleotide probes using template-free DNA polymerases.