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1.WO/2022/106522ENZYMATIC MONOCYCLIZATION OF ACYCLIC MONOTERPENOIDS
WO 27.05.2022
Int.Class C12N 9/88
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
88Lyases (4.)
Appl.No PCT/EP2021/082104 Applicant UNIVERSITÄT STUTTGART Inventor HAUER, Bernhard
Enzyme mutant with squalene-hopene-cyclase activity, selected from mutants of a wild-type enzyme comprising an amino acid sequence selected from SEQ-ID No: 1 to 3 or an amino acid sequence derived therefrom with a degree of sequence identity in the range of from 60 to 99,9 % of SEQ-ID No. 1 to 3, wherein the mutant catalyzes a one-step monocyclization reaction to produce products such as gamma-dihydroionone and/or alpha-dihydroionone.
2.WO/2022/105640NUCLEIC ACID SEQUENCING METHOD
WO 27.05.2022
Int.Class C12Q 1/6869
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6869Methods for sequencing
Appl.No PCT/CN2021/129461 Applicant TSINGHUA UNIVERSITY Inventor BAI, Jingwei
The present invention provides a nucleic acid sequencing method, comprising: providing a polymerase linked to dNTP by means of a cleavable group and capable of emitting an optical signal; making the polymerase in contact with 3' end of a primer of a nucleic acid template-primer complex to be measured, complementarily pairing dNTP on the polymerase with a base group on a nucleic acid sequence to be measured, and adding the 3' end of the primer into a polymerase enzymatic reaction; and detecting the optical signal emitted by the polymerase. Subsequently, dNTP and the polymerase are broken, and the new template-primer complex after the leaving of the polymerase may enter the next sequencing cycle.
3.WO/2022/109194METHODS OF IMPROVING PRODUCTION OF MORPHINAN ALKALOIDS AND DERIVATIVES
WO 27.05.2022
Int.Class C12N 1/19
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
14Fungi ; Culture media therefor
16Yeasts; Culture media therefor
19modified by introduction of foreign genetic material
Appl.No PCT/US2021/059980 Applicant ANTHEIA, INC. Inventor SMOLKE, Christina, D.
Methods and engineered cells are provided for increasing activity of a norcoclaurine synthase in a microbial cell. The method comprises, within the engineered microbial cell, contacting an engineered norcoclaurine synthase with a substrate, wherein contacting the substrate with the engineered norcoclaurine synthase increases conversion, within the engineered microbial cell, in comparison to a non-engineered norcoclaurine synthase.
4.WO/2022/108383MICROORGANISM HAVING ENHANCED L-GLUTAMINE PRODUCING ABILITY, AND L-GLUTAMINE PRODUCING METHOD USING SAME
WO 27.05.2022
Int.Class C12N 15/77
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
77for Corynebacterium; for Brevibacterium
Appl.No PCT/KR2021/017074 Applicant CJ CHEILJEDANG CORPORATION Inventor CHOI, Su Jin
The present application relates to a microorganism having enhanced L-glutamine producing ability, and an L-glutamine producing method using same.
5.WO/2022/104673METHOD FOR PRODUCING ARACHIDONIC ACID
WO 27.05.2022
Int.Class C12P 7/64
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
7Preparation of oxygen-containing organic compounds
64Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
Appl.No PCT/CN2020/130322 Applicant INNER MONGOLIA KINGDOMWAY PHARMACEUTICAL LIMITED Inventor WANG, Xiaofeng
The invention belongs to the field of fermentation engineering, and relates to a method for producing arachidonic acid. The method comprises: successively subjecting Mortierella alpine or mutant strains thereof to strain activation, seed amplification culture and fermentation culture, wherein OUR is monitored online, and the OUR value is controlled at 10-100 mmol/L.h during the fermentation culture, and/or after 100 h of fermentation culture, ORP is monitored in fermentation liquid online, and the ORP value is controlled to be within the range of 50-150 mv. By adopting the method, the fermentation production level can be stably improved, such that the content of the arachidonic acid in the obtained fermentation solution is obviously improved.
6.WO/2022/107388PROCESSED PRODUCT CONTAINING MUSHROOMS OF THE GENUS FLAMMULINA AND METHOD FOR MANUFACTURING SAME
WO 27.05.2022
Int.Class C12P 1/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
1Preparation of compounds or compositions, not provided for in groups C12P3/-C12P39/121; General processes for the preparation of compounds or compositions by using microorganisms or enzymes
Appl.No PCT/JP2021/026727 Applicant KAGOME CO., LTD. Inventor TSUNOI Tatsuto
[Problem] To provide an umami enhancing composition in which mushroom odor is suppressed. A strong mushroom odor and low amount of umami-enhancing components have been inherent in conventional processed products using mushrooms. Seasoning has been required to strengthen umami-enhancing components while suppressing mushroom odor in processed products using mushrooms. [Solution] An umami-enhancing composition in which mushroom odor was suppressed was successfully provided by carrying out an enzyme treatment on a processed product of mushrooms of the genus Flammulina using at least one or more of deaminase or glutaminase.
7.WO/2022/106638METHODS FOR PRODUCTION OF CIS-TRANS-NEPETALACTOL AND IRIDOIDS
WO 27.05.2022
Int.Class C12N 9/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02Oxidoreductases (1.), e.g. luciferase
Appl.No PCT/EP2021/082339 Applicant DANMARKS TEKNISKE UNIVERSITET Inventor JENSEN, Michael Krogh
The present invention relates to microbial factories, in particular yeast cell factories, for production of cis-trans-nepetalactol and optionally other plant-derived compounds, such as iridoids. Also provided are methods for producing cis-trans-nepetalactol in a yeast cell, as well as useful nucleic acids, vectors and host cells.
8.WO/2022/105482METHOD FOR PREPARING NICOTINE OF HIGH OPTICAL PURITY
WO 27.05.2022
Int.Class C07D 401/04
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
DHETEROCYCLIC COMPOUNDS
401Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
02containing two hetero rings
04directly linked by a ring-member-to-ring- member bond
Appl.No PCT/CN2021/123322 Applicant SHANDONG JINCHENG PHARMACCUTICAL CHEMICAL CO., LTD Inventor LI, Jiaquan
Disclosed is a method for preparing nicotine of a high optical purity, wherein biological enzyme catalysis technology is used to achieve directional reduction of an intermediate product, myosmine; the intermediate product myosmine can be reduced and hydrogenated and converted into a single chirality; and L-nicotine of a high yield and a high optical purity is then obtained by means of a methylation reaction. The L-nicotine has a chiral purity of up to 99%, and can be directly used for preparing downstream products. Enzymes used can be efficiently recycled, such that the cost is greatly reduced.
9.WO/2022/105841USE OF FATTY ACID ELONGASE GENE AND ESTERASE GENE IN SYNTHESIS OF NERVONIC ACID AND GREASE IN YEAST
WO 27.05.2022
Int.Class C12N 1/19
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
14Fungi ; Culture media therefor
16Yeasts; Culture media therefor
19modified by introduction of foreign genetic material
Appl.No PCT/CN2021/131563 Applicant QINGDAO INSTITUTE OF BIOENERGY AND BIOPROCESS TECHNOLOGY, CHINESE ACADEMY OF SCIENCES Inventor WANG, Shian
Provided is an engineering bacterium for producing nervonic acid and/or grease. The genome of the engineering bacterium is integrated with an expression cassette expressing a protein encoded by 3-ketoacyl-CoA synthase (KCS) gene and/or an esterase gene.
10.WO/2022/104460OLIVETOLIC ACID CYCLASE VARIANTS WITH IMPROVED ACTIVITY FOR USE IN PRODUCTION OF PHYTOCANNABINOIDS
WO 27.05.2022
Int.Class C12N 15/60
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
11DNA or RNA fragments; Modified forms thereof
52Genes encoding for enzymes or proenzymes
60Lyases (4)
Appl.No PCT/CA2021/051626 Applicant HYASYNTH BIOLOGICALS INC. Inventor LIAO, Timothy S.
The present disclosure relates generally to methods, isolated polypeptides and polynucleotides, expression vectors, and host cells for the production of olivetolic acid and phytocannabinoids. A method of producing olivetolic acid (OVLa) and/or a phytocannabinoid in a heterologous host cell having OVLa-producing or phytocannabinoid-producing capacity comprises transforming the host cell with a nucleotide encoding a variant olivetolic acid cyclase (OAC) protein having at least 6 amino acid mutations relative to the wild type OAC protein, and culturing the transformed host cell to produce OVLa and/or phytocannabinoids therefrom. The variant OAC protein (SEQ ID NO:92) has at least 85% sequence identity with the wild type OAC protein (SEQ ID NO:91). Exemplary variants having improved OVLa or phytocannabinoid production capacity are described.