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IC:C12P19/34

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Analysis

1 / 1,723
1.WO/2025/147660MODIFIED RNA FOR INCREASING PROTEIN EXPRESSION
WO - 10.07.2025
Int.Class C12N 15/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
 Appl.No PCT/US2025/010301 Applicant TRILINK BIOTECHNOLOGIES, LLC Inventor XU, Chunping
Described herein are modified RNA molecules where a 3'-stabilizing region is covalently attached to the RNA, and where the 3'-stabilizing region comprises one or more modified nucleosides. Methods of synthesizing said RNAs are also provided herein.
2.20250223620NOVEL PROCESSES FOR THE PRODUCTION OF POLYNUCLEOTIDES INCLUDING OLIGONUCLEOTIDES
US - 10.07.2025
Int.Class C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
 Appl.No 18853613 Applicant GLAXOSMITHKLINE INTELLECTUAL PROPERTY DEVELOPMENT LIMITED Inventor Andreas CRAMERI

The invention relates to novel processes using enzymes for the production of polynucleotides or oligonucleotides, wherein said processes are suitable for use in the production of modified polynucleotides or oligonucleotides, such as those for use in therapy.

3.20250223621COMPOSITIONS AND METHODS FOR RNA SYNTHESIS
US - 10.07.2025
Int.Class C12P 19/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
19Preparation of compounds containing saccharide radicals
26Preparation of nitrogen-containing carbohydrates
28N-glycosides
30Nucleotides
34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
 Appl.No 18908683 Applicant Helix Nanotechnologies Inc Inventor Nikhil Dhar

Compositions and methods for synthesizing an RNA product are provided herein. For example, the present disclosure provides a method of producing an RNA product comprising incubating an in vitro transcription mixture, thereby producing an RNA product that comprises a plurality of single-stranded RNA molecules. In some embodiments, an in vitro transcription mixture comprises a DNA template comprising an RNA polymerase promoter sequence operatively linked to a target sequence; at least one RNA polymerase that recognizes the RNA polymerase promoter sequence; a plurality of ribonucleotides comprising at least two different types of ribonucleotides, each type comprising a different nucleoside; and a transcription buffer comprising an osmolyte.

4.4153241CONJUGATION DEVICE AND METHOD FOR PRODUCING CONJUGATES
PL - 07.07.2025
Int.Class C12M 1/40
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
40Apparatus specially designed for the use of free, immobilised, or carrier-bound enzymes, e.g. apparatus containing a fluidised bed of immobilised enzymes
 Appl.No 21925332 Applicant Inventor GANG QIN
5.WO/2025/141025NOVEL CAPPING STRATEGIES FOR MRNA 5'CAP
WO - 03.07.2025
Int.Class C07H 19/16
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
19Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro derivatives thereof
02sharing nitrogen
04Heterocyclic radicals containing only nitrogen as ring hetero atom
16Purine radicals
 Appl.No PCT/EP2024/088312 Applicant ELEVEN THERAPEUTICS LTD Inventor DELABARRE, Byron Scott
The present invention relates to synthetic cap analogs useful for the functionalization of mRNAs, which can replace canonical cap structures. Compounds disclosed herein may be functionalized with a polynucleotide using chemical synthesis approaches.
6.WO/2025/137875ISOLATED POLYPEPTIDE, PREPARATION METHOD THEREFOR, AND USE THEREOF
WO - 03.07.2025
Int.Class C12N 9/12
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
 Appl.No PCT/CN2023/142063 Applicant BGI SHENZHEN Inventor LIU, Shanshan
The present application provides an isolated polypeptide, a preparation method therefor, and use thereof. The polypeptide has an amino acid sequence that is at least 99% homologous with SEQ ID NO:1, and that is preferably 100% homologous therewith.
7.WO/2025/138041T4 DNA LIGASE MUTANT AND USE THEREOF
WO - 03.07.2025
Int.Class C12N 9/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
 Appl.No PCT/CN2023/142951 Applicant BGI SHENZHEN Inventor XIE, Qingqing
Provided are a T4 DNA ligase mutant and the use thereof. The T4 DNA ligase mutant: a) has an amino acid sequence having at least 80% identity to SEQ ID NO: 1; b) comprises a substitution of an amino acid at the position corresponding to position 28 of SEQ ID NO: 1; and c) has a T4 DNA ligase activity. The T4 DNA ligase mutant has improved double-end linker connection efficiency and single-chain cyclization performance, and is compatible with different reaction systems. The T4 DNA ligase mutant can be widely used in library construction for various high-throughput sequencing, single-chain cyclization and ligation of DNA fragments in molecular cloning.
8.WO/2025/137876ISOLATED POLYPEPTIDE, PREPARATION METHOD, AND USE
WO - 03.07.2025
Int.Class C12N 9/12
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
 Appl.No PCT/CN2023/142067 Applicant BGI SHENZHEN Inventor LI, Denghui
Provided in the present application are an isolated polypeptide, a preparation method, and a use. The polypeptide has an amino acid sequence which is at least 91% homologous or 92% homologous or 93% homologous or 94% homologous or 95% homologous or 96% homologous or 97% homologous or 98% homologous or 99% homologous to SEQ ID NO: 1.
9.WO/2025/144716NUCLEOTIDES WITH ENZYMATICALLY CLEAVABLE 3'-O-GLYCOSIDE BLOCKING GROUPS FOR SEQUENCING
WO - 03.07.2025
Int.Class C07H 19/067
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
19Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro derivatives thereof
02sharing nitrogen
04Heterocyclic radicals containing only nitrogen as ring hetero atom
06Pyrimidine radicals
067with ribosyl as the saccharide radical
 Appl.No PCT/US2024/061310 Applicant ILLUMINA, INC. Inventor EMOND, Stephane
Embodiments of the present disclosure relate to nucleotide and nucleoside molecules with 3'-O-glycoside blocking groups. Also provided herein are methods to prepare such nucleotide and nucleoside molecules, and methods and kits for sequencing applications.
10.WO/2025/142849MODIFIED RNA POLYMERASE
WO - 03.07.2025
Int.Class C12N 9/12
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
10Transferases (2.)
12transferring phosphorus containing groups, e.g. kinases (2.7)
 Appl.No PCT/JP2024/045489 Applicant TOYOBO CO., LTD. Inventor OCHI, Kensuke
The present invention addresses the problem of providing a novel modified RNA polymerase. The problem is solved by a modified RNA polymerase in which at least one amino acid residue at a specific position is modified.
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