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Analysis

1.WO/2021/207293SCALABLE BIOREACTOR SYSTEMS AND RELATED METHODS OF USE
WO 14.10.2021
Int.Class C12M 3/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
04with means providing thin layers
Appl.No PCT/US2021/026071 Applicant MISSION BARNS, INC. Inventor ENGELMAYR, George C. Jr.
Bioreactors configured to scale-up the production of greater quantities of cells at relatively low cost are provided. These bioreactors may be utilized in the production of large-scale quantities of cell-based meat and cell-based fat. The bioreactors may be reusable and may have a high surface area-to-volume ratio for adherent cell expansion. The bioreactors may be capable of yielding a large number of adherent cells per bioreactor unit.
2.WO/2021/206555FLUIDIC DEVICE, CELL CULTURING SYSTEM AND METHOD OF TESTING A COMPOUND
WO 14.10.2021
Int.Class B01L 3/00
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
Appl.No PCT/NL2021/050231 Applicant UNIVERSITEIT TWENTE Inventor DE SÁ VIVAS, Aisen Gabriel
A fluidic device, in particular a microfluidic chip, for culturing a 3D cell culture comprises a bottom wall defining a bottom side of an open-top chamber arranged for containing a cell culture medium. A fluidic channel extends below the chamber between a channel inlet and a channel outlet for guiding a fluid flow. The bottom wall is provided with a micro-opening through which the chamber and the microfluidic channel are in fluid communication. A side wall extends from the bottom wall circumferentially around the chamber to a top wall opposite the bottom wall. An upper edge of the side wall delimits an open access upper side of the chamber and the top wall is arranged for closing the chamber at the upper side thereof and to be supported by the upper edge of the side wall. A sealing means is provided between opposing surfaces of the upper edge of the side wall and the top wall and arranged for reversible fluid sealing of the side wall and top wall when the chamber is closed at the upper side by the top wall.
3.WO/2021/206752SYSTEM AND METHOD FOR SENSING, CAPTURE AND RELEASE OF BIOMOLECULES OR CELLS
WO 14.10.2021
Int.Class C12M 1/34
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
34Measuring or testing with condition measuring or sensing means, e.g. colony counters
Appl.No PCT/US2020/055931 Applicant INDIANA BIOSCIENCES RESEARCH INSTITUTE, INC. Inventor PUGIA, Michael, J.
Collection of target analytes from complex samples is performed in detection microwell which includes a size exclusion filter and allows incubating the target analyte with affinity agents for a target analyte for capture and removal of non-target molecules. Detection of the target analytes by collection of the complexes in close proximity to a working electrode and a reference electrode facilitates the detection electrochemical labels produced.
4.WO/2021/204940MULTI-WELL PLATES FOR CREATION OF HIGH-DENSITY ARRAYS OF COMPARTMENTALIZED CELL CULTURES FOR USAGE IN HIGH CAPACITY APPLICATIONS
WO 14.10.2021
Int.Class C12M 1/32
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
26Inoculator or sampler
32multiple field or continuous type
Appl.No PCT/EP2021/059170 Applicant CELLECTRICON AB Inventor PIHL, Johan
The disclosure relates to multi-well plates having fluidic connections between neighboring wells that are useful to produce a cell culture substrate and compliant with American National Standards Institute of the Society for Laboratory Automation and Screening (ANSI/SLAS) microplate standards.
5.20210316367FABRICATION OF POROUS SCAFFOLDS USING ADDITIVE MANUFACTURING WITH POTENTIAL APPLICATIONS IN BONE TISSUE ENGINEERING
US 14.10.2021
Int.Class B22F 10/38
BPERFORMING OPERATIONS; TRANSPORTING
22CASTING; POWDER METALLURGY
FWORKING METALLIC POWDER; MANUFACTURE OF ARTICLES FROM METALLIC POWDER; MAKING METALLIC POWDER; APPARATUS OR DEVICES SPECIALLY ADAPTED FOR METALLIC POWDER
10Additive manufacturing of workpieces or articles from metallic powder
30Process control
38to achieve specific product aspects, e.g. surface smoothness, density, porosity or hollow structures
Appl.No 17266807 Applicant Ohio State Innovation Foundation Inventor Hernan Lara PADILLA

The present disclosure describes additive manufacturing methods, in particular methods for optimizing the manufacture of objects by three-dimensional printing processes. Also provided are bioreactors as well as methods of using thereof.

6.20210316302MICROFLUIDIC DEVICE MIMICKING A BIOLOGICAL MICROENVIRONMENT AND COMPRISING DIFFUSION CHAMBERS AND CHANNELS
US 14.10.2021
Int.Class B01L 3/00
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
3Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
Appl.No 17086462 Applicant KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY Inventor Seung Beum SUH

The present disclosure relates to a microfluidic device capable of mimicking a biological microenvironment thereby achieving control of a microenvironment with time through control of the diffusion of target elements between chambers by interrupting or generating fluid flow inside a channel, and a method for manufacturing the same, and the microfluidic device according to an aspect of the present disclosure can mimic the biological microenvironment which changes constantly with time via a simple temporary operation of generating or interrupting fluid flow in the channel mechanically and can control diffusion via a simple method, and accordingly, a complicated microenvironment can be reproduced since the number and arrangement of diffusion chambers and channels can be designed variously.

7.WO/2021/206055UNIT FOR ANGIOGENESIS PROMOTION AND/OR NERVE REGENERATION
WO 14.10.2021
Int.Class A61P 9/00
AHUMAN NECESSITIES
61MEDICAL OR VETERINARY SCIENCE; HYGIENE
PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
9Drugs for disorders of the cardiovascular system
Appl.No PCT/JP2021/014496 Applicant ICHIMARU PHARCOS CO., LTD. Inventor MASUTANI Teruaki
[Problem] To provide a safe means for angiogenesis promotion and/or nerve regeneration (for example, a technique for inducing angiogenesis in transplanted cells or a tissue in vivo) in order to meet requirements for the development of the aforesaid means, a material such as a scaffold enabling the post-transplantation engraftment and growth of nerve stem cells (tissue stem cells of undifferentiated nervous system), etc. [Solution] A unit to be used for angiogenesis promotion and/or nerve regeneration, said unit comprising a gel component and a proteoglycan, etc.
8.WO/2021/206551MICROFLUIDIC DEVICE FOR A 3D TISSUE STRUCTURE
WO 14.10.2021
Int.Class C12M 3/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
3Tissue, human, animal or plant cell, or virus culture apparatus
Appl.No PCT/NL2021/050227 Applicant RIVER BIOMEDICS B.V. Inventor CATARINO RIBEIRO, Marcelo
Microfluidic device comprising at least one cell culture unit for forming, culturing, growing and/or maintaining a 3D tissue structure such as a 3D strip of cardiac tissue, wherein the at least one cell culture unit comprises: a respective culture chamber for culturing cells having a chamber outlet opening; and a cell supply channel arranged to guide a microfluidic flow of liquid holding cells between a channel inlet and a channel outlet, wherein the cell supply channel is provided with a flow inhibitor which is operable to selectively provide a flow inhibiting state or a flow permitting state depending on a fluid pressure at the flow inhibitor, wherein, in the flow inhibiting state, the flow inhibitor is configured to substantially inhibit liquid flow between the cell supply channel and the culture chamber, wherein, in the flow permitting state, the flow inhibitor is configured to permit such liquid flow such that the cell supply channel is in liquid communication with the culture chamber to supply the culture chamber with cells, wherein the culture chamber is provided with at least two mutually spaced apart elastic support structures which extend in the culture chamber and which are configured for elastically supporting a tissue formed in the culture chamber, in particular a cultured 3D tissue formed from the cells, wherein the elastic support structures are elastically deformable, in particular flexible, in particular to vary a mutual distance of said support structures under influence of a varying contraction force between said support structures.
9.WO/2021/206686MICROFLUIDIC CHIP CELL SORTING AND TRANSFECTION
WO 14.10.2021
Int.Class C12M 1/12
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
12with sterilisation, filtration, or dialysis means
Appl.No PCT/US2020/026989 Applicant HEWLETT-PACKARD DEVELOPMENT COMPANY, L.P. Inventor SHKOLNIKOV, Viktor
A cell transfection apparatus may include a microfluidic chip. The microfluidic chip may include a fluid input port, a cell sorter to sort target cells from non-target cells in fluid received through the input port, a cell transfection region comprising a single cell electroporation region to receive the target cells sorted from the nontarget cells and a fluid ejector to dispense a transfected target cell received from the cell transfection region.
10.WO/2021/207766THREE-DIMENSIONAL BIOREACTOR FOR VIRAL VECTOR PRODUCTION
WO 14.10.2021
Int.Class C12N 5/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
02Propagation of single cells or cells in suspension; Maintenance thereof; Culture media therefor
Appl.No PCT/US2021/070371 Applicant SOUTHWEST RESEARCH INSTITUTE Inventor LING, Jian
The present disclosure relates to the design, fabrication, and applications of a three-dimensional (3D) bioreactor for expansion of viral vector producing cells and ultimate harvesting of viral vectors. The bioreactor is composed of non-random interconnected voids providing a continuous three-dimensional surface area for cell adherence and growth. The 3D bioreactor is also scalable with a defined geometry, surface coating, and fluidic dynamics to maintain a monolayer cell culture and reduce or prevent cell aggregation, phenotype change, or extracellular production, and is particularly suitable for culturing of HEK 293T cells providing lentiviral vectors under appropriate surface coating of the bioreactor. The invention can also extend to produce other types of viruses and vaccines based on live-attenuated viruses, or inactivated viruses, or viral vectors.