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1. US20080066530 - Method for the optimization of chromatographic purification processes for biological molecules

Office United States of America
Application Number 11762119
Application Date 13.06.2007
Publication Number 20080066530
Publication Date 20.03.2008
Grant Number 08349615
Grant Date 08.01.2013
Publication Kind B2
IPC
G01N 30/02
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
02Column chromatography
G01N 30/38
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
02Column chromatography
26Conditioning of the fluid carrier; Flow patterns
38Flow patterns
Applicants Boehringer Ingelheim Pharma GmbH & Co. KG
Inventors Eckermann Christian
Ebert Sybille
Rubenwolf Stefanie
Ambrosius Dorothee
Agents Morris Michael P.
Lebel Eduoard G.
Title
(EN) Method for the optimization of chromatographic purification processes for biological molecules
Abstract
(EN)

The invention relates to a method of finding suitable parameters for the chromatographic purification of biomolecules. The method consists of equilibration, charging, washing and eluting steps, this sequence of steps being carried out by the partial batch method. The parameters determined in small, preferably numerous parallel test batches provide conclusions as to the chromatography conditions under which a given biomolecule can be purified optimally by column chromatography, optionally even on a larger scale.