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1. US20060096924 - Method for discovering suitable chromatography conditions for the separation of biological molecules

Office United States of America
Application Number 10528103
Application Date 19.09.2003
Publication Number 20060096924
Publication Date 11.05.2006
Publication Kind A1
IPC
B01D 15/08
BPERFORMING OPERATIONS; TRANSPORTING
01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
DSEPARATION
15Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
08Selective adsorption, e.g. chromatography
G01N 30/00
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
G01N 30/46
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
02Column chromatography
26Conditioning of the fluid carrier; Flow patterns
38Flow patterns
46using more than one column
G01N 30/86
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
30Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography
02Column chromatography
86Signal analysis
Applicants SCHLUETER HARTMUT
Inventors Schlueter Hartmut
Agents MILLEN, WHITE, ZELANO &; BRANIGAN, P.C.
Priority Data 10243529 19.09.2002 DE
Title
(EN) Method for discovering suitable chromatography conditions for the separation of biological molecules
Abstract
(EN)

The invention relates to a multiparallel chromatography system for developing a method for the purification of proteins and other biomolecules. The system uses cavities of multiwell plates (for example 96 well plates) filled with chromatography gels, and consists of diverse start buffers in which the samples to be chromatographed are dissolved and with which the gels are equilibrated, and solutions for desorption (elution) of the biomolecules bound to the gels. The system comprises aids for interpretation of the results obtained from the post-chromatography analyses (for example protein concentration determinations, bioassays, etc.) of the supernatants of the unbound biomolecules and/or the eluates. These aids may also consist of programs into which the results are entered and which, after processing of the results, supply interpretations and proposals for the design of the steps for the purification of a biomolecule.

Also published as
DE10393819