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1. JP1999108932 - METHOD FOR DETECTION OR MEASUREMENT OF VIRUS

Office Japan
Application Number 1998218136
Application Date 31.07.1998
Publication Number 1999108932
Publication Date 23.04.1999
Grant Number 3171827
Grant Date 23.03.2001
Publication Kind B2
IPC
G01N 33/569
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
C12P 21/08
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
08Monoclonal antibodies
G01N 33/531
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
531Production of immunochemical test materials
G01N 33/543
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
543with an insoluble carrier for immobilising immunochemicals
G01N 33/577
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
577involving monoclonal antibodies
C12R 1/91
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1Microorganisms
91Cell lines
Applicants TONEN CORP
株式会社先端生命科学研究所
Inventors AOYANAGI KATSUMI
青柳 克己
OUE CHIHARU
大植 千春
IIDA KUMIKO
飯田 久美子
KIMURA TATSUJI
木村 達治
YAGI SHINTARO
八木 慎太郎
Agents 石田 敬
福本 積
西山 雅也
Priority Data 1997209522 04.08.1997 JP
Title
(EN) METHOD FOR DETECTION OR MEASUREMENT OF VIRUS
(JA) ウイルスの検出又は測定方法
Abstract
(EN)

PROBLEM TO BE SOLVED: To realize a virus antigen detection method which is suitable for treating many samples by a method wherein a sample which contains a virus is treated with a treatment solution which contains one out of an anionic surface-active agent, an amphoteric ionic surface-active agent, a nonionic surface-active agent and a protein denaturant.

SOLUTION: A virus as an object to be measured is a virus which forms virus particles having a structure which is constituted of a structure protein which contains genomic RNA or DNA and of a membrane protein or a lipid membrane surrounding the protein. A biological humor such as whole blood, plasma, serum, urine, saliva, a cerebrospinal fluid or the like and a liver tissue or the like are included in a sample to be measured. When a treatment agent which contains anionic surface-active agent such as SDS or the like is added to the sample to be measured, the virus is destroyed, an anticore antibody in the sample is denatured, and a core antigen in the sample is isolated. Alternatively, when an amphoteric ionic surface-active agent or a protein denaturant which displayed an action to destroy the higher-order structure of a protein is added in addition to the anionic surface-active agent, a denaturing action with reference to the solid-phase antibody of SDS is measured, and the sensitivity of the detection or the measurement of the virus is increased.

COPYRIGHT: (C)1999,JPO

(JA)


【課題】 ウイルスを簡便で高感度に検出または測定す
る方法の提供。


【解決手段】 ウイルスを含む検体を、(1)陰イオン
性界面活性剤、及び(2)両イオン性界面活性剤、非イ
オン性界面活性剤又は蛋白質変性剤のいずれかを含む処
理液で処理することを特徴とするウイルス含有検体の処
理方法;並びにこの方法の実施のためのモノクローナル
抗体及びそれを生産するハイブリドーマ。