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1. JP1999051940 - DETECTING/MEASURING METHOD FOR HCV

Office Japan
Application Number 1997209515
Application Date 04.08.1997
Publication Number 1999051940
Publication Date 26.02.1999
Grant Number 3176570
Grant Date 06.04.2001
Publication Kind B2
IPC
G01N 33/576
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
576for hepatitis
G01N 33/577
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
577involving monoclonal antibodies
C12N 5/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
C12N 15/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
02Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
C12P 21/08
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
21Preparation of peptides or proteins
08Monoclonal antibodies
Applicants TONEN CORP
株式会社先端生命科学研究所
Inventors AOYANAGI KATSUMI
青柳 克己
OUE CHIHARU
大植 千春
KIMURA TATSUJI
木村 達治
IIDA KUMIKO
飯田 久美子
YAGI SHINTARO
八木 慎太郎
Agents 石田 敬
福本 積
戸田 利雄
西山 雅也
Title
(EN) DETECTING/MEASURING METHOD FOR HCV
(JA) HCVの検出又は測定方法
Abstract
(EN)

PROBLEM TO BE SOLVED: To simply detect and quantify HCV with high sensitivity by processing a specimen containing HCV with a processing solution containing chaotropic ions and an acidifier.

SOLUTION: The problem of acid treatment such as the formation of precipitates and the contradictory problem of guanidine treatment are resolved when acid treatment and guanidine treatment are combined. When a surfactant is added to a treating agent generating chaotropic ions such as guanidine and a treating agent constituted of an acidifier, a further favorable result is obtained. Hydrochloric acid, sulfuric acid, acetic acid, trifluoroacetic acid, and trichloroacetic acid are suitable for the acidifier. A specimen containing virus grains such as HCV is processed by these methods, and a virus antigen can be simply detected and quantified with good sensitivity by the so-called immunoassay detecting the antigen with an antibody used as a probe.

COPYRIGHT: (C)1999,JPO

(JA)


【課題】 HCVを簡便で高感度に検出または測定する
方法の提供。


【解決手段】 HCVを含む検体を、(1)カオトロピ
ックイオン、及び(2)酸性化剤を含む処理液で処理す
ることを特徴とするHCV含有検体の処理方法;並びに
この方法の実施のためのモノクローナル抗体及びそれを
生産するハイブリドーマ。