Processing

Please wait...

Settings

Settings

Goto Application

1. EP3465216 - PRETREATMENT METHOD FOR RAPID DETECTION OF HCV CORE ANTIGEN

Office European Patent Office
Application Number 17728121
Application Date 31.05.2017
Publication Number 3465216
Publication Date 10.04.2019
Publication Kind B1
IPC
G01N 33/576
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
576for hepatitis
CPC
G01N 33/5767
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
576for hepatitis
5767non-A, non-B hepatitis
G01N 33/84
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
84involving inorganic compounds or pH
Applicants ROCHE DIAGNOSTICS GMBH
HOFFMANN LA ROCHE
Inventors UPMEIER BARBARA
ZARNT TORALF
GINTER MANFRED
BOLLHAGEN RALF
Designated States
Priority Data 16172155 31.05.2016 EP
2017063077 31.05.2017 EP
Title
(DE) VORBEHANDLUNGSVERFAHREN ZUR SCHNELLEN ERKENNUNG VON HCV-KERNANTIGEN
(EN) PRETREATMENT METHOD FOR RAPID DETECTION OF HCV CORE ANTIGEN
(FR) PROCÉDÉ DE PRÉTRAITEMENT POUR LA DÉTECTION RAPIDE D'ANTIGÈNE CAPSIDIQUE DU VIRUS DE L'HÉPATITE C
Abstract
(EN)
The present invention relates to a method for detecting acore polypeptide of a hepatitis C virus (HCV) in a sample from a subject comprising (a) contacting said sample with a surfactant comprising a cationic detergent; (b) contacting said sample with a binding com- pound; and (c) detecting a core polypeptide of said HCV in said sample; wherein step a) is immediately followed by step b). The present invention further relates to a method for pre- processing a sample from a subject for detection of an HCV core polypeptide, comprising (a) contacting said sample with a surfactant comprising a cationic detergent and, optionally, with an agent inducing a pH shift, immediately followed by (b) contacting said sample with a binding compound. Moreover, the present invention further relates to uses, devices, and analytical systems related to aforesaid methods.

(FR)
La présente invention concerne un procédé de détection d'un polypeptide de noyau d'un virus de l'hépatite C (VHC) dans un échantillon prélevé d'un sujet consistant en : a) la mise en contact dudit échantillon avec un tensioactif comprenant un détergent cationique ; (b) la mise en contact dudit échantillon avec un composé de liaison ; et (c) la détection d'un polypeptide de noyau dudit VHC dans ledit échantillon ; l'étape a) étant immédiatement suivie de l'étape b). La présente invention concerne en outre un procédé de prétraitement d'un échantillon provenant d'un sujet pour la détection d'un polypeptide de noyau d'un VHC, consistant en (a) la mise en contact dudit échantillon avec un tensioactif comprenant un détergent cationique et, facultativement, avec un agent induisant un décalage de pH, immédiatement suivi par (b) la mise en contact dudit échantillon avec un composé de liaison. En outre, la présente invention concerne des utilisations, des dispositifs et des systèmes analytiques associés aux procédés mentionnés ci-dessus.