Processing

Please wait...

Settings

Settings

Goto Application

1. EP0967484 - METHODS FOR DETECTING OR ASSAYING VIRUS

Office European Patent Office
Application Number 98935359
Application Date 04.08.1998
Publication Number 0967484
Publication Date 29.12.1999
Publication Kind B1
IPC
G01N 33/569
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
G01N 33/576
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
576for hepatitis
CPC
G01N 33/5767
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
576for hepatitis
5767non-A, non-B hepatitis
G01N 33/56983
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
56983Viruses
G01N 2333/02
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
005from viruses
01DNA viruses
02Hepadnaviridae, e.g. hepatitis B virus
G01N 2333/18
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
2333Assays involving biological materials from specific organisms or of a specific nature
005from viruses
08RNA viruses
18Togaviridae; Flaviviridae
Applicants ADVANCED LIFE SCIENCE INST INC
Inventors AOYAGI KATSUMI
OHUE CHIHARU
IIDA KUMIKO
KIMURA TATSUJI
YAGI SHINTARO
Designated States
Priority Data 1997209515 04.08.1997 JP
20952297 04.08.1997 JP
1998218136 31.07.1998 JP
9803476 04.08.1998 JP
Title
(DE) METHODEN ZUM NACHWEIS UND ZUR ANALYSE VON VIRUS
(EN) METHODS FOR DETECTING OR ASSAYING VIRUS
(FR) METHODES PERMETTANT DE DETECTER OU D'ANALYSER UN VIRUS
Abstract
(EN)
A method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) an anionic surfactant and (2) an amphoteric surfactant, nonionic surfactant or protein denaturant; a virus assay method using said treating method; a method for treating a virus-containing sample, characterized by treatment of a virus-containing sample with a treatment solution containing (1) a chaotropic ion and (2) an acidifying agent; a virus assay method using said treating method; a virus assay method, characterized in that a virus antigen and a virus antibody are measured based on their binding to their probe in the presence of a surfactant with an alkyl group of 10 or more carbon atoms and a secondary, tertiary or quaternary amine, or a nonionic surfactant, or of both of them; and a monoclonal antibody and a hybridoma producing the same for carrying out said method.

(FR)
L'invention concerne une méthode qui permet de traiter un échantillon contenant un virus, caractérisée par le fait que l'on traite ledit échantillon avec une solution de traitement contenant (1) un tensioactif anionique et (2) un tensioactif amphotère, un tensioactif non ionique ou un agent de dénaturation des protéines; une méthode qui permet d'analyser un virus par ladite méthode de traitement; une méthode qui permet de traiter un échantillon contenant un virus, caractérisée par le fait que l'on traite ledit échantillon avec une solution de traitement contenant (1) un ion chaotropique et (2) un agent acidifiant; une méthode qui permet d'analyser un virus par ladite méthode de traitement; une méthode qui permet d'analyser un virus, caractérisée par le fait que l'on analyse un antigène viral et un anticorps viral en présence d'un tensioactif comportant un groupe alkyle avec 10 atomes de carbone ou davantage et une amine secondaire, tertiaire ou quaternaire et/ou un tensioactif non ionique, sur la base de la fixation sur les sondes correspondantes; un anticorps monoclonal qui permet de mettre en oeuvre ladite méthode et un hybridome qui produit ledit anticorps.