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1. CA2493240 - METHODS FOR DETECTING OR ASSAYING VIRUS

Office Canada
Application Number 2493240
Application Date 04.08.1998
Publication Number 2493240
Publication Date 11.02.1999
Publication Kind A1
IPC
C12N 5/12
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
12Fused cells, e.g. hybridomas
C07K 16/10
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins, e.g. monoclonal or polyclonal antibodies
08against material from viruses
10from RNA viruses
C12N 7/06
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
04Inactivation or attenuation; Producing viral sub-units
06by chemical treatment
G01N 33/569
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
569for microorganisms, e.g. protozoa, bacteria, viruses
G01N 33/576
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/-G01N31/131
48Biological material, e.g. blood, urine; Haemocytometers
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
576for hepatitis
Applicants ADVANCED LIFE SCIENCE INSTITUTE, INC.
Inventors AOYAGI, KATSUMI
OHUE, CHIHARU
IIDA, KUMIKO
KIMURA, TATSUJI
YAGI, SHINTARO
Priority Data 10/218136 31.07.1998 JP
9/209515 04.08.1997 JP
9/209522 04.08.1997 JP
Title
(EN) METHODS FOR DETECTING OR ASSAYING VIRUS
(FR) METHODES PERMETTANT DE DETECTER OU D'ANALYSER UN VIRUS
Abstract
(EN)
A method for treating a hepatitis C or hepatitis B virus-containing sample, characterized by treatment of the virus-containing sample with a treatment solution containing (1) an anionic surfactant and (2) an amphoteric surfactant, nonionic surfactant or protein denaturant, and a virus assay method using said treating method. Also provided is a method for treating a hepatitis C virus-containing sample, characterized by treatment of the virus-containing sample with a treatment solution containing (1) a chaotropic ion and (2) an acidifying agent, and a virus assay method using said treating method. Further provided is a hepatitis C virus assay method comprising treating a hepatitis C virus antigen-containing sample with a surfactant having an alkyl group of 10 or more carbon atoms and a secondary, tertiary or quaternary amine, or a nonionic surfactant, or of both of them, and measuring binding of the antigen with a hepatitis C virus antibody. Finally, there is also provided a monoclonal antibody and a hybridoma producing the same for carrying out the above methods.