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1. WO2022252745 - KIT FOR DETECTION OF VIRUS PARTICLES USING IMMUNE MOLECULES

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Publication Number WO/2022/252745
Publication Date 08.12.2022
International Application No. PCT/CN2022/080846
International Filing Date 15.03.2022
IPC
C12N 7/02 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses, e.g. bacteriophages; Compositions thereof; Preparation or purification thereof
02Recovery or purification
C12N 1/20 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
C12Q 1/70 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
70involving virus or bacteriophage
C12Q 1/68 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
C12R 1/93 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C-C12Q75
1Microorganisms
92Viruses
93Animal viruses
CPC
C12N 1/20
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof
20Bacteria
C12N 2710/00
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
2710dsDNA Viruses
C12N 7/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
7Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
02Recovery or purification
C12Q 1/68
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
70involving virus or bacteriophage
Applicants
  • 重庆医科大学 CHONGQING MEDICAL UNIVERSITY [CN]/[CN]
Inventors
  • 汪德强 WANG, Deqiang
  • 伍晓莉 WU, Xiaoli
  • 刘俊叶 LIU, Junye
  • 马园艳 MA, Yuanyan
  • 蔡雪飞 CAI, Xuefei
  • 黄爱龙 HUANG, Ailong
  • 王雯 WANG, Wen
  • 毛胜蓝 MAO, Shenglan
  • 沈仕梅 SHEN, Shimei
Agents
  • 重庆智慧之源知识产权代理事务所（普通合伙） CHONGQING ZHZY INTELLECTUAL PROPERTY AGENCY , GP
Priority Data
202110613870.602.06.2021CN
202111212401.X18.10.2021CN
202210027310.711.01.2022CN
Publication Language Chinese (zh)
Filing Language Chinese (ZH)
Designated States
Title
(EN) KIT FOR DETECTION OF VIRUS PARTICLES USING IMMUNE MOLECULES
(FR) KIT DE DÉTECTION DE PARTICULES VIRALES À L'AIDE DE MOLÉCULES IMMUNITAIRES
(ZH) 一种免疫分子病毒颗粒检测试剂盒
Abstract
(EN) A kit for the detection of virus particles using immune molecules. A monoclonal antibody of a virus envelope antigen is subjected to biotin modification, and a magnetic bead is coupled with streptavidin; then, the biotin-modified monoclonal antibody is incubated with a virus-containing solution, the antibody and virus particles or an antigen form a complex, then, the streptavidin coupled magnetic bead is added for incubation, and the streptavidin on the magnetic bead binds, with high specificity and high affinity, to the biotin coupled antibody so as to specifically capture a virus particle with the envelope; and after a supernatant is separated by using a magnetic separator, the complete virus particle, an empty capsid virus and a free envelope antigen can be separated from other virus components, and then the magnetic bead conjugate is qualitatively or quantitatively detected by means of PCR amplification.
(FR) L'invention concerne un kit pour la détection de particules virales à l'aide de molécules immunitaires. Un anticorps monoclonal d'un antigène d'enveloppe virale est soumis à une modification à la biotine, et une bille magnétique est couplée à la streptavidine; puis, l'anticorps monoclonal modifié à la biotine est incubé avec une solution contenant un virus, l'anticorps et les particules virales ou un antigène forment un complexe, puis, la bille magnétique couplée à la streptavidine est ajoutée pour l'incubation, et la streptavidine sur la bille magnétique se lie, avec une haute spécificité et une haute affinité, à l'anticorps couplé à la biotine de façon à capturer spécifiquement une particule virale avec l'enveloppe; et après la séparation d'un surnageant à l'aide d'un séparateur magnétique, la particule virale complète, un virus à capside vide et un antigène d'enveloppe libre peuvent être séparés des autres composants du virus, puis le conjugué à bille magnétique est détecté qualitativement ou quantitativement au moyen d'une amplification par PCR.
(ZH) 一种免疫分子病毒颗粒检测试剂盒，将病毒包膜抗原的单克隆抗体进行生物素修饰，将磁珠与链霉亲和素进行偶联；然后将生物素修饰的单克隆抗体与含有病毒的溶液进行孵育，抗体与病毒颗粒或抗原形成复合物，然后加入已经偶联链霉亲和素的磁珠进行孵育，磁珠上的链霉亲和素将与偶联了生物素的抗体进行高特异高亲和力结合，进而特异性地捕获具有包膜的病毒颗粒，再通过磁分离器分离上清液后，完整病毒颗粒及空壳病毒、游离的包膜抗原可以与其它病毒组分分离，然后通过PCR扩增对磁珠结合物进行定性或者定量检测。
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