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1. WO2022206661 - NUCLEIC ACID ISOTHERMAL AMPLIFICATION METHOD AND APPLICATION THEREOF

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Publication Number WO/2022/206661
Publication Date 06.10.2022
International Application No. PCT/CN2022/083311
International Filing Date 28.03.2022
IPC
C12Q 1/6844 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
C12N 15/09 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
C12N 9/22 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14Hydrolases (3.)
16acting on ester bonds (3.1)
22Ribonucleases
CPC
C12N 15/09
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
C12N 9/22
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9Enzymes; Proenzymes; Compositions thereof
14Hydrolases (3)
16acting on ester bonds (3.1)
22Ribonucleases ; RNAses, DNAses
C12Q 1/6844
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
C12Q 1/70
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
70involving virus or bacteriophage
Applicants
  • 杭州杰毅生物技术有限公司 HANGZHOU MATRIDX BIOTECHNOLOGY CO., LTD. [CN]/[CN]
Inventors
  • 韩序 HAN, Xu
  • 段昆 DUAN, Kun
  • 高晓庆 GAO, Xiaoqing
  • 王珺 WANG, Jun
Agents
  • 北京华睿卓成知识产权代理事务所（普通合伙） CHENG & PENG INTELLECTUAL PROPERTY LAW OFFICE
Priority Data
202110338236.630.03.2021CN
Publication Language Chinese (zh)
Filing Language Chinese (ZH)
Designated States
Title
(EN) NUCLEIC ACID ISOTHERMAL AMPLIFICATION METHOD AND APPLICATION THEREOF
(FR) PROCÉDÉ D'AMPLIFICATION ISOTHERME D'ACIDE NUCLÉIQUE ET SON APPLICATION
(ZH) 一种核酸等温扩增方法及其应用
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Abstract
(EN) Disclosed are a nucleic acid isothermal amplification method and an application thereof. a reverse transcriptase, an RNA polymerase, and an RNase H used in the method are all heat-resistant enzymes still active at at least 45 °C, and an amplification reaction is performed at a constant temperature of not less than 45 °C. The isothermal nucleic acid amplification method of the present invention has the characteristics of being rapid, isothermal, highly sensitive, and highly specific, and is suitable for nucleic acid detection in a field such as clinical testing.
(FR) La présente invention concerne un procédé d'amplification isotherme d'acide nucléique et son application. Une transcriptase inverse, une ARN polymérase et une RNase H utilisées dans le procédé sont toutes des enzymes résistantes à la chaleur, encore actives à au moins 45 °C, et une réaction d'amplification est réalisée à une température constante non inférieure à 45 °C. Le procédé d'amplification isotherme de l'acide nucléique de la présente invention se distingue par sa rapidité, son caractère isotherme, sa grande sensibilité et sa grande spécificité, et convient à la détection de l'acide nucléique dans un domaine tel que les tests cliniques.
(ZH) 本发明公开了一种核酸的恒温扩增方法及其应用。该方法中所用的逆转录酶、RNA聚合酶以及RNA酶H均为至少在45℃仍具有活性的耐热酶，并且所述扩增反应在不低于45℃的温度下恒温进行。本发明的恒温核酸扩增方法具有快速、恒温、灵敏度和特异性高的特点，适合临床检验等领域的核酸检测。
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