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1. WO2022165353 - SEQUENCE CONVERSION AND SIGNAL AMPLIFIER DNA HAVING ABASIC NUCLEIC ACIDS, AND DETECTION METHODS USING SAME

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Publication Number WO/2022/165353
Publication Date 04.08.2022
International Application No. PCT/US2022/014590
International Filing Date 31.01.2022
IPC
C12Q 1/68 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
C12Q 1/6844 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
C12Q 1/6886 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
6883for diseases caused by alterations of genetic material
6886for cancer
CPC
C12Q 1/682
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
6816characterised by the detection means
682Signal amplification
Applicants
  • ABBOTT LABORATORIES [US]/[US]
Inventors
  • MORIKAWA, Takamitsu
  • YOSHIMURA, Toru
Agents
  • SINGER, Christopher P.
Priority Data
63/144,14601.02.2021US
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) SEQUENCE CONVERSION AND SIGNAL AMPLIFIER DNA HAVING ABASIC NUCLEIC ACIDS, AND DETECTION METHODS USING SAME
(FR) ADN DE CONVERSION DE SÉQUENCE ET AMPLIFICATEUR DE SIGNAL COMPORTANT DES ACIDES NUCLÉIQUES ABASIQUES ET PROCÉDÉS DE DÉTECTION L'UTILISANT
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Abstract
(EN) Disclosed are methods for detecting a target nucleic acid in a sample. The methods include contacting the sample, in the presence of a polymerase and an endonuclease, with a first oligonucleotide that includes, in the 5' to 3' direction, a signal DNA generation sequence, an endonuclease recognition site, and a complementary sequence that has at least one abasic moiety and wherein the complementary sequence has a first complementary sequence that is complementary to at least a portion of the signal DNA generation sequence and a second complementary sequence that is complementary to the 3' end of the target nucleic acid. Also disclosed are methods that include a second oligonucleotide including, a second signal DNA generation sequence, an endonuclease recognition site, and a sequence that is homologous to the first signal DNA generation sequence of the first oligonucleotide and that optionally has at least one abasic site.
(FR) La présente invention concerne des procédés pour détecter un acide nucléique cible dans un échantillon. Les procédés comprennent la mise en contact de l'échantillon, en présence d'une polymérase et d'une endonucléase, avec un premier oligonucléotide qui comprend, dans le sens 5' vers 3 ', une séquence de génération d'ADN de signal, un site de reconnaissance d'endonucléase, et une séquence complémentaire qui comporte au moins une fraction abasique et la séquence complémentaire comportant une première séquence complémentaire qui est complémentaire d'au moins une partie de la séquence de génération d'ADN de signal et une seconde séquence complémentaire qui est complémentaire à l'extrémité 3' de l'acide nucléique cible. L'invention concerne également des procédés qui comprennent un second oligonucléotide comprenant une seconde séquence de génération d'ADN de signal, un site de reconnaissance d'endonucléase et une séquence qui est homologue à la première séquence de génération d'ADN de signal du premier oligonucléotide et qui a facultativement au moins un site abasique.
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