WO2022163936 NOVEL EXCINUCLEASE ABC SUBUNIT A VARIANT, AND METHOD FOR PRODUCING L-GLUTAMIC ACID USING SAME

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Publication Number WO/2022/163936

Publication Date 04.08.2022

International Application No. PCT/KR2021/005155

International Filing Date 23.04.2021

IPC

CPC

Applicants

씨제이제일제당 (주) CJ CHEILJEDANG CORPORATION [KR]/[KR]

Inventors

박고운 PARK, Goun
배지연 BAE, Jee Yeon
권나라 KWON, Nara
김효진 KIM, Hyo Jin
이진남 LEE, Jin Nam
서창일 SEO, Chang Il

Agents

특허법인한얼 HANOL INTELLECTUAL PROPERTY AND LAW

Priority Data

10-2021-0011243

27.01.2021

Publication Language Korean (ko)

Filing Language Korean (KO)

Designated States

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Title

(EN) NOVEL EXCINUCLEASE ABC SUBUNIT A VARIANT, AND METHOD FOR PRODUCING L-GLUTAMIC ACID USING SAME

(FR) NOUVEAU VARIANT DE LA SOUS-UNITÉ A DE L'EXCINUCLÉASE ABC, ET PROCÉDÉ DE PRODUCTION D'ACIDE L-GLUTAMIQUE FAISANT APPEL À CELUI-CI

(KO) 신규한 엑시뉴클레아제 ABC 서브유닛 A 변이체 및 이를 이용한 L-글루탐산 생산 방법

Abstract

(EN) The present application relates to a novel excinuclease ABC subunit A variant, a Corynebacterium glutamicum strain comprising the variant, and a method for producing L-glutamic acid using the strain.

(FR) La présente invention concerne un nouveau variant de la sous-unité A de l'excinucléase ABC, une souche de Corynebacterium glutamicum comprenant ledit variant, et un procédé de production d'acide L-glutamique faisant appel à ladite souche.

(KO) 본 출원은 신규한 엑시뉴클레아제 ABC 서브유닛 A 변이체, 상기 변이체를 포함하는 코리네박테리움 글루타미쿰 균주 및 상기 균주를 이용한 L-글루탐산 생산 방법에 관한 것이다.

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14	Hydrolases (3.)
16	acting on ester bonds (3.1)

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74	Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
77	for Corynebacterium; for Brevibacterium

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P	FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13	Preparation of nitrogen-containing organic compounds
04	Alpha- or beta-amino acids
14	Glutamic acid; Glutamine

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
14	Hydrolases (3.)
16	acting on ester bonds (3.1)

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74	Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
77	for Corynebacterium; for Brevibacterium

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1. WO2022163936 - NOVEL EXCINUCLEASE ABC SUBUNIT A VARIANT, AND METHOD FOR PRODUCING L-GLUTAMIC ACID USING SAME