WO2022163926 NOVEL PROLINE DEHYDROGENASE VARIANT AND METHOD FOR PRODUCING L-VALINE USING SAME

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Publication Number WO/2022/163926

Publication Date 04.08.2022

International Application No. PCT/KR2021/004522

International Filing Date 09.04.2021

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Applicants

씨제이제일제당 (주) CJ CHEILJEDANG CORPORATION [KR]/[KR]

Inventors

배지연 BAE, Jee Yeon
김선혜 KIM, Seon Hye
윤병훈 YOON, Byoung Hoon
박고운 PARK, Goun
김효진 KIM, Hyo Jin
김형준 KIM, Hyung Joon

Agents

유미특허법인 YOU ME PATENT AND LAW FIRM

Priority Data

10-2021-0011015

26.01.2021

Publication Language Korean (ko)

Filing Language Korean (KO)

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Title

(EN) NOVEL PROLINE DEHYDROGENASE VARIANT AND METHOD FOR PRODUCING L-VALINE USING SAME

(FR) NOUVEAU VARIANT DE PROLINE DÉSHYDROGÉNASE ET PROCÉDÉ DE PRODUCTION DE L-VALINE L'UTILISANT

(KO) 신규한 프롤린 탈수소효소 변이체 및 이를 이용한 L-발린 생산 방법

Abstract

(EN) The present application relates to a novel proline dehydrogenase variant, a Corynebacterium glutamicum strain comprising the variant, and a method for producing L-valine using the strain.

(FR) La présente demande concerne un nouveau variant de proline déshydrogénase, une souche de Corynebacterium glutamicum comprenant le variant, et un procédé de production de L-valine utilisant la souche.

(KO) 본 출원은 신규한 프롤린 탈수소효소 변이체, 상기 변이체를 포함하는 코리네박테리움 글루타미쿰 균주 및 상기 균주를 이용한 L-발린 생산 방법에 관한 것이다.

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02	Oxidoreductases (1.), e.g. luciferase
06	acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74	Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
77	for Corynebacterium; for Brevibacterium

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
P	FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
13	Preparation of nitrogen-containing organic compounds
04	Alpha- or beta-amino acids
08	Lysine; Diaminopimelic acid; Threonine; Valine

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
9	Enzymes, e.g. ligases (6.); Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating, or purifying enzymes
02	Oxidoreductases (1.), e.g. luciferase
06	acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
N	MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15	Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09	Recombinant DNA-technology
63	Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
74	Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
77	for Corynebacterium; for Brevibacterium

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1. WO2022163926 - NOVEL PROLINE DEHYDROGENASE VARIANT AND METHOD FOR PRODUCING L-VALINE USING SAME

C	CHEMISTRY; METALLURGY
12	BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
Y	ENZYMES
105	Oxidoreductases acting on the CH-NH group of donors (1.5)
99	with other acceptors (1.5.99)
99008	Proline dehydrogenase (1.5.99.8)