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1. WO2022117884 - TOMATO PLANTS RESISTANT TO TOBRFV, TMV, TOMV AND TOMMV AND CORRESPONDING RESISTANCE GENES

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Publication Number WO/2022/117884
Publication Date 09.06.2022
International Application No. PCT/EP2021/084289
International Filing Date 03.12.2021
IPC
A01H 1/00 2006.1
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1Processes for modifying genotypes
C12N 15/82 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
CPC
A01H 1/126
AHUMAN NECESSITIES
01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
HNEW PLANTS OR ; NON-TRANSGENIC; PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
1Processes for modifying genotypes ; ; Plants characterised by associated natural traits;
12Processes for modifying agronomic input traits, e.g. crop yield
122for stress resistance, e.g. heavy metal resistance
1245for biotic stress resistance, e.g. pathogen, pest or disease resistance
126for virus resistance
C12N 15/8283
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells ; , e.g. plant artificial chromosomes (PACs)
8241Phenotypically and genetically modified plants via recombinant DNA technology
8261with agronomic (input) traits, e.g. crop yield
8271for stress resistance, e.g. heavy metal resistance
8279for biotic stress resistance, pathogen resistance, disease resistance
8283for virus resistance
Applicants
  • VILMORIN & CIE [FR]/[FR]
Inventors
  • LINDBO, John
Agents
  • ERNEST GUTMANN
Priority Data
20306496.903.12.2020EP
21306078.302.08.2021EP
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) TOMATO PLANTS RESISTANT TO TOBRFV, TMV, TOMV AND TOMMV AND CORRESPONDING RESISTANCE GENES
(FR) PLANTS DE TOMATE RÉSISTANTS AU TOBRFV, AU TMV, AU TOMV ET AU TOMMV, ET GÈNES DE RÉSISTANCE CORRESPONDANTS
Abstract
(EN) Tomato plants resistant to ToBRFV, TMV, ToMV and ToMMV and corresponding resistance genes The invention relates to a variants of the TM-2-2 protein, conferring recognition of the Movement Protein (MP) of the Tomato Brown Rugose Fruit Virus (ToBRFV), and wherein said variant comprises a tyrosine (Y), a phenylalanine (F) or a tryptophan (W) at the position corresponding to tyrosine 767 of the TM-2-2 protein and at least one of the following mutations: C848R, N822C, N822F, N822M, N822Y, N822W, N825H, N825K and N825T with respect to the TM-2-2 protein, potentially in combination with a F655L mutation. The present invention also relates to genetic sequences encoding such a variant protein, preferably to a mutated Tm-2-2 gene, and to plants, especially Solanum lycopersicum plants comprising in their genome said mutated gene conferring resistance to ToBRFV. The invention is also directed to parts of these plants, as well as progeny, and to the use of these sequences for providing ToBRFV resistance.
(FR) L’invention concerne des plants de tomate résistants au ToBRFV, au TMV, au ToMV et au ToMMV et les gènes de résistance correspondants. L'invention concerne une variante de la protéine TM-2-2 conférant la reconnaissance de la protéine de mouvement (MP) du virus du fruit rugueux brun de tomate (ToBRFV), et ladite variante comprenant une tyrosine (Y), une phénylalanine (F) ou un tryptophane (W) à la position correspondant à la tyrosine 767 de la protéine TM-2-2, et au moins l'une des mutations suivantes : C848R, N822C, N822F, N822M, N822Y, N822W, N825H, N825K et N825T par rapport à la protéine TM-2-2, potentiellement en combinaison avec une mutation F655L. La présente invention concerne également des séquences génétiques codant pour une telle protéine variante, de préférence un gène Tm-2-2 muté, et des plants, en particulier des plants de Solanum lycopersicum comprenant dans leur génome ledit gène muté conférant une résistance au ToBRFV. L'invention concerne également des parties de ces plants, ainsi qu'une descendance, et l'utilisation de ces séquences pour fournir une résistance au ToBRFV.
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