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1. WO2022116782 - ETHYL MALTOL MONOCLONAL ANTIBODY HYBRIDOMA CELL LINE AND APPLICATION THEREOF

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Publication Number WO/2022/116782
Publication Date 09.06.2022
International Application No. PCT/CN2021/129534
International Filing Date 09.11.2021
IPC
C12N 5/20 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
5Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
10Cells modified by introduction of foreign genetic material, e.g. virus-transformed cells
12Fused cells, e.g. hybridomas
16Animal cells
18Murine cells, e.g. mouse cells
20one of the fusion partners being a B lymphocyte
C07K 16/44 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins, e.g. monoclonal or polyclonal antibodies
44against material not provided for elsewhere
C07K 14/795 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
795Porphyrin- or corrin-ring-containing peptides
C07K 14/765 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
76Albumins
765Serum albumin, e.g. HSA
C07K 1/02 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General processes for the preparation of peptides
02in solution
C07K 1/107 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
1General processes for the preparation of peptides
107by chemical modification of precursor peptides
CPC
C07D 309/40
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
DHETEROCYCLIC COMPOUNDS
309Heterocyclic compounds containing six-membered rings having one oxygen atom as the only ring hetero atom, not condensed with other rings
34having three or more double bonds between ring members or between ring members and non-ring members
36with oxygen atoms directly attached to ring carbon atoms
40Oxygen atoms attached in positions 3 and 4, e.g. maltol
C07K 14/765
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
435from animals; from humans
76Albumins
765Serum albumin, e.g. HSA
C07K 14/795
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
795Porphyrin- or corrin-ring-containing peptides
C07K 16/44
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
16Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
44against material not provided for elsewhere ; , e.g. haptens, metals, DNA, RNA, amino acids
G01N 33/5308
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
5308for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
G01N 33/577
GPHYSICS
01MEASURING; TESTING
NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
33Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
48Biological material, e.g. blood, urine
50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
53Immunoassay; Biospecific binding assay; Materials therefor
577involving monoclonal antibodies ; binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens;
Applicants
  • 江南大学 JIANGNAN UNIVERSITY [CN]/[CN]
Inventors
  • 胥传来 XU, Chuanlai
  • 雷咸禄 LEI, Xianlu
  • 匡华 KUANG, Hua
  • 徐丽广 XU, Liguang
  • 马伟 MA, Wei
  • 刘丽强 LIU, Liqiang
  • 吴晓玲 WU, Xiaoling
  • 宋珊珊 SONG, Shanshan
  • 胡拥明 HU, Yongming
Agents
  • 无锡承果知识产权代理有限公司 CHENGGUO INTELLECTUAL PROPERTY AGENCY CO., LTD
Priority Data
202011397292.904.12.2020CN
Publication Language Chinese (zh)
Filing Language Chinese (ZH)
Designated States
Title
(EN) ETHYL MALTOL MONOCLONAL ANTIBODY HYBRIDOMA CELL LINE AND APPLICATION THEREOF
(FR) LIGNÉE CELLULAIRE D'HYBRIDOME D'ANTICORPS MONOCLONAL D'ÉTHYL-MALTOL ET APPLICATION ASSOCIÉE
(ZH) 一株乙基麦芽酚单克隆抗体杂交瘤细胞株及其应用
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Abstract
(EN) An ethyl maltol monoclonal antibody hybridoma cell line ABC01 and an application thereof in the analysis and detection of ethyl maltol residues in food safety testing. The hybridoma cell line was deposited in the China General Microbiological Culture Collection Center (CGMCC) on 28 November 2019 under the deposit number CGMCC No. 19178. A monoclonal antibody secreted by the cell line has good specificity and detection sensitivity with regard to ethyl maltol, and can achieve the detection of ethyl maltol residues in oils and fats, milk, and other foods, providing raw materials for immunodetection of the ethyl maltol residues in said foods.
(FR) L'invention concerne une lignée cellulaire d'hybridome d'anticorps monoclonal d'éthyl-maltol ABC01 et une application de celle-ci dans l'analyse et la détection de résidus d'éthyl-maltol dans un test de salubrité alimentaire. La lignée cellulaire d'hybridome a été déposée au Centre général de collecte chinois de cultures microbiologiques (CGMCC) le 28 novembre 2019 sous le numéro de dépôt CGMCC No. 19178. Un anticorps monoclonal sécrété par la lignée cellulaire présente une bonne spécificité et une bonne sensibilité de détection vis-à-vis de l'éthyl-maltol et peut réaliser la détection de résidus d'éthyl-maltol dans des huiles et des graisses, du lait et d'autres aliments, fournissant des matières premières pour l'immunodétection de résidus d'éthyl-maltol dans lesdits aliments.
(ZH) 一株乙基麦芽酚单克隆抗体杂交瘤细胞株ABC01及其在食品安全检测中乙基麦芽酚残留的分析检测中的应用，该杂交瘤细胞株保藏于中国微生物菌种保藏管理委员会普通微生物中心CGMCC，保藏日期2019年11月28日，保藏编号CGMCC No.19178。此细胞株分泌的单克隆抗体，对乙基麦芽酚具有较好的特异性和检测灵敏度，可实现对油脂、牛奶等食品中乙基麦芽酚残留量的检测，为食品中乙基麦芽酚残留的免疫检测提供了原料。
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