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1. WO2022088477 - NUCLEIC ACID DETECTOR AND NUCLEIC ACID DETECTION METHOD

Publication Number WO/2022/088477
Publication Date 05.05.2022
International Application No. PCT/CN2020/140787
International Filing Date 29.12.2020
IPC
C12M 1/34 2006.1
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MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
1Apparatus for enzymology or microbiology
34Measuring or testing with condition measuring or sensing means, e.g. colony counters
C12M 1/00 2006.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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1Apparatus for enzymology or microbiology
C12Q 1/6844 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6844Nucleic acid amplification reactions
C12Q 1/6806 2018.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction assay
C12Q 1/6813 2018.1
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12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6813Hybridisation assays
CPC
C12Q 1/6806
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
C12Q 1/6813
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6813Hybridisation assays
C12Q 1/6844
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6844Nucleic acid amplification reactions
Applicants
  • 广州和实生物技术有限公司 GUANGZHOU HEAS BIOTECH CO., LTD. [CN]/[CN]
Inventors
  • 陈华云 CHEN, Huayun
  • 杨迎宾 YANG, Yingbin
  • 邹天桥 ZOU, Tianqiao
  • 薛儒冰 XUE, Rubing
  • 肖湘文 XIAO, Xiangwen
  • 刘淑园 LIU, Shuyuan
  • 吕婧 LV, Jing
  • 曾烨 ZENG, Ye
Agents
  • 北京品源专利代理有限公司 BEYOND ATTORNEYS AT LAW
Priority Data
202011165266.327.10.2020CN
Publication Language Chinese (zh)
Filing Language Chinese (ZH)
Designated States
Title
(EN) NUCLEIC ACID DETECTOR AND NUCLEIC ACID DETECTION METHOD
(FR) DISPOSITIF DE DÉTECTION D'ACIDE NUCLÉIQUE ET PROCÉDÉ DE DÉTECTION D'ACIDE NUCLÉIQUE
(ZH) 核酸检测仪及核酸检测方法
Abstract
(EN) The present application belongs to the technical field of biological detection, and particularly discloses a nucleic acid detector and a nucleic acid detection method. The nucleic acid detector comprises: a mounting rack; a reagent plate, provided with a nucleic acid amplification tube, a hybridization tank, a reaction tube and reagent tubes for storing and extracting the reagent; a cover plate assembly, capable of moving in a direction toward or away from the nucleic acid amplification tube, so as to close or open the tube opening of the nucleic acid amplification tube; a heat treatment unit, used for heating or cooling the liquid on the reagent plate; a pipetting device, used for aspirating and dispensing the liquid on the reagent plate; an oscillating unit, used for driving the reagent plate to oscillate reciprocally in the horizontal direction; a magnetic attraction assembly, used for attracting and releasing magnetic beads in the reaction liquid on the reagent plate. The nucleic acid detection method uses the described nucleic acid detector to perform nucleic acid detection. The present nucleic acid detector and nucleic acid detection method can improve the efficiency of nucleic acid detection.
(FR) La présente invention se rapporte au domaine technique de la détection biologique, et concerne en particulier un dispositif de détection d'acide nucléique et un procédé de détection d'acide nucléique. Le dispositif de détection d'acide nucléique comprend les éléments suivants : un support de montage ; une plaque de réactif, pourvue d'un tube d'amplification d'acide nucléique, d'un réservoir d'hybridation, d'un tube de réaction et de tubes de réactif pour stocker et extraire le réactif ; un ensemble de plaque de couverture, capable de se déplacer dans une direction vers ou à l'écart du tube d'amplification d'acide nucléique, de manière à fermer ou ouvrir l'ouverture du tube d'amplification d'acide nucléique ; une unité de traitement thermique, utilisée pour chauffer ou refroidir le liquide sur la plaque de réactif ; un dispositif de pipetage, utilisé pour aspirer et distribuer le liquide sur la plaque de réactif ; une unité d'oscillation, utilisée pour entraîner la plaque de réactif afin qu'elle oscille en va-et-vient dans la direction horizontale ; un ensemble d'attraction magnétique, utilisé pour attirer et libérer des billes magnétiques dans le liquide de réaction sur la plaque de réactif. Le procédé de détection d'acide nucléique utilise le détecteur d'acide nucléique décrit pour effectuer une détection d'acide nucléique. Le présent détecteur d'acide nucléique et le procédé de détection d'acide nucléique peuvent améliorer l'efficacité de la détection d'acide nucléique.
(ZH) 本申请属于生物检测技术领域,具体公开了一种核酸检测仪和核酸检测方法。核酸检测仪包括:安装架;试剂板,试剂板上设置有核酸扩增管、杂交槽、反应管及存放提取试剂的试剂管;盖板组件,能够沿朝向或远离核酸扩增管的方向运动,以封闭或开放核酸扩增管的管口;热处理单元,用于对试剂板上液体进行加热或冷却处理;移液装置,用于对试剂板上的液体进行吸取及转移;振荡单元,用于带动试剂板沿水平方向往复振荡;磁吸组件,磁吸组件用于对试剂板上的反应液中的磁珠进行吸磁和放磁。核酸检测方法采用上述核酸检测仪进行核酸检测。本申请提供的核酸检测仪和核酸检测方法,能够提高核酸检测的效率。
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