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1. WO2022074380 - BACTERIAL MICROCOMPARTMENTAL SHELLS

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Publication Number WO/2022/074380
Publication Date 14.04.2022
International Application No. PCT/GB2021/052574
International Filing Date 05.10.2021
IPC
C12N 1/20 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
20Bacteria; Culture media therefor
C07K 17/02 2006.1
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
17Carrier-bound or immobilised peptides; Preparation thereof
02Peptides being immobilised on, or in, an organic carrier
C12N 15/82 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
79Vectors or expression systems specially adapted for eukaryotic hosts
82for plant cells
CPC
C07K 14/195
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
14Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
195from bacteria
C07K 17/02
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
17Carrier-bound or immobilised peptides
02Peptides being immobilised on, or in, an organic carrier
C07K 2319/01
CCHEMISTRY; METALLURGY
07ORGANIC CHEMISTRY
KPEPTIDES
2319Fusion polypeptide
01containing a localisation/targetting motif
C12N 1/20
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
1Microorganisms, e.g. protozoa; Compositions thereof
20Bacteria
C12N 11/02
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
11Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
02Enzymes or microbial cells immobilised on or in an organic carrier
C12N 11/04
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
11Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
02Enzymes or microbial cells immobilised on or in an organic carrier
04entrapped within the carrier, e.g. gel or hollow fibres
Applicants
  • THE UNIVERSITY OF LIVERPOOL [GB]/[GB]
Inventors
  • LIU, Luning
  • LI, Tianpei
  • JIANG, Qiuyao
  • HUANG, Fang
Agents
  • TLIP LTD
Priority Data
2015810.106.10.2020GB
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) BACTERIAL MICROCOMPARTMENTAL SHELLS
(FR) ENVELOPPES MICROCOMPARTIMENTÉES BACTÉRIENNES
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Abstract
(EN) The present invention relates to new synthetically generated carboxysome shells, shells encapsulating selected catalytically active enzymes, cells including the shells and methods of making the shells and methods of encapsulation. The invention also includes uses of the cells as nanoreactors for enhanced catalytic performance of the encapsulated enzymes and the use of an encapsulation protein for recruiting external cargo proteins into a synthetic carboxysome shell.
(FR) La présente invention concerne de nouvelles enveloppes de carboxysome générées de manière synthétique, des enveloppes encapsulant des enzymes catalytiquement actives sélectionnées, des cellules comprenant les enveloppes et des procédés de fabrication des enveloppes et des procédés d'encapsulation. L'invention concerne également des utilisations des cellules en tant que nanoréacteurs afin d’améliorer les performances catalytiques des enzymes encapsulées et l'utilisation d'une protéine d'encapsulation pour recruter des protéines cargo externes dans une enveloppe de carboxysome synthétique.
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