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1. WO2022067494 - METHOD FOR DETECTION OF WHOLE TRANSCRIPTOME IN SINGLE CELLS

Publication Number WO/2022/067494
Publication Date 07.04.2022
International Application No. PCT/CN2020/118794
International Filing Date 29.09.2020
IPC
C12Q 1/6869 2018.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
68involving nucleic acids
6869Methods for sequencing
C12N 15/10 2006.1
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C40B 70/00 2006.1
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40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
70Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or barcodes
CPC
C12N 15/10
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
15Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
09Recombinant DNA-technology
10Processes for the isolation, preparation or purification of DNA or RNA
C12Q 1/6869
CCHEMISTRY; METALLURGY
12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
1Measuring or testing processes involving enzymes, nucleic acids or microorganisms
68involving nucleic acids
6869Methods for sequencing
C40B 70/00
CCHEMISTRY; METALLURGY
40COMBINATORIAL TECHNOLOGY
BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
70Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
Applicants
  • SINGLERON (NANJING) BIOTECHNOLOGIES, LTD. [CN]/[CN]
Inventors
  • SUN, Jiameng
  • TANG, Guoxin
  • LI, Ji
  • LI, Tao
Agents
  • BEYOND ATTORNEYS AT LAW
Priority Data
Publication Language English (en)
Filing Language English (EN)
Designated States
Title
(EN) METHOD FOR DETECTION OF WHOLE TRANSCRIPTOME IN SINGLE CELLS
(FR) PROCÉDÉ DE DÉTECTION DE TRANSCRIPTOME ENTIER DANS DES CELLULES INDIVIDUELLES
Abstract
(EN) Provided is a method, based on the Singleron Independent developed Microfluidic Chip to separate single cells, using oligo dT and N6 magnetic beads to capture poly A+ RNA and poly A-RNA, and the captured RNA is than reverse transcribed to cDNA. The resulting cDNA can be amplified and analyzed. Whole transcriptome sequencing at the massively parallel single cells level, provides more useful data for the analysis of cell expression patterns and regulatory mechanisms.
(FR) L'invention concerne un procédé, basé sur la puce microfluidique développée par Singleron Independent pour séparer des cellules uniques, utilisant des billes magnétiques oligo dT et N6 pour capturer l'ARN poly A+ et l'ARN poly A, et l'ARN capturé est ensuite transcrit de manière inverse en ADNc. L'ADNc résultant peut alors être amplifié et analysé. Le séquençage du transcriptome entier au niveau des cellules individuelles massivement parallèles fournit des données plus utiles pour l'analyse de profils d'expression cellulaire et de mécanismes de régulation.
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