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1. WO2022043438 - METHOD, IMAGE PROCESSING UNIT AND LASER SCANNING MICROSCOPE FOR BACKGROUND-REDUCED IMAGING OF A STRUCTURE IN A SPECIMEN

Publication Number WO/2022/043438
Publication Date 03.03.2022
International Application No. PCT/EP2021/073615
International Filing Date 26.08.2021
IPC
G02B 21/00 2006.1
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
G02B 21/36 2006.1
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
36arranged for photographic purposes or projection purposes
G02B 27/58 2006.1
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
27Optical systems or apparatus not provided for by any of the groups G02B1/-G02B26/119
58Optics for apodization or superresolution; Optical synthetic aperture systems
G06T 5/00 2006.1
GPHYSICS
06COMPUTING; CALCULATING OR COUNTING
TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
5Image enhancement or restoration
CPC
G02B 21/0076
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
0004specially adapted for specific applications
002Scanning microscopes
0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
0052Optical details of the image generation
0076arrangements using fluorescence or luminescence
G02B 21/008
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
0004specially adapted for specific applications
002Scanning microscopes
0024Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
008Details of detection or image processing, including general computer control
G02B 21/367
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
21Microscopes
36arranged for photographic purposes or projection purposes
365Control or image processing arrangements for digital or video microscopes
367providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
G02B 27/58
GPHYSICS
02OPTICS
BOPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
27Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
58Optics for apodization or superresolution; Optical synthetic aperture systems
G06T 2207/10056
GPHYSICS
06COMPUTING; CALCULATING; COUNTING
TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
2207Indexing scheme for image analysis or image enhancement
10Image acquisition modality
10056Microscopic image
G06T 2207/20221
GPHYSICS
06COMPUTING; CALCULATING; COUNTING
TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
2207Indexing scheme for image analysis or image enhancement
20Special algorithmic details
20212Image combination
20221Image fusion; Image merging
Applicants
  • ABBERIOR INSTRUMENTS GMBH [DE]/[DE]
Inventors
  • WEIDLING, Frederic
  • HEINE, Jörn
  • KASTRUP, Lars
Priority Data
10 2020 122 605.528.08.2020DE
Publication Language German (de)
Filing Language German (DE)
Designated States
Title
(DE) VERFAHREN, BILDVERARBEITUNGSEINHEIT UND LASERSCANNINGMIKROSKOP ZUM HINTERGRUNDREDUZIERTEN ABBILDEN EINER STRUKTUR IN EINER PROBE
(EN) METHOD, IMAGE PROCESSING UNIT AND LASER SCANNING MICROSCOPE FOR BACKGROUND-REDUCED IMAGING OF A STRUCTURE IN A SPECIMEN
(FR) PROCÉDÉ, UNITÉ DE TRAITEMENT D'IMAGE ET MICROSCOPE À BALAYAGE LASER POUR IMAGERIE À ARRIÈRE-PLAN RÉDUIT D'UNE STRUCTURE DANS UN ÉCHANTILLON
Abstract
(DE) Es wird ein Verfahren, eine Bildverarbeitungsvorrichtung und ein Laserscanningmikroskop zum Abbilden einer mit einem Fluoreszenzfarbstoff markierten Struktur in einer Probe, wobei (störender) Bildhintergrund gegenüber Verfahren aus dem Stand der Technik reduziert sind, beschrieben. Die Erfindung zeichnet sich dadurch aus, dass das Fluoreszenzsignal mit mehreren, in einer Detektionsfläche angeordneten Detektorelementen detektiert wird und dass aus den Signalen der Detektorelemente Rohbilder berechnet werden, die gegenüber den Detektorsignalen reduzierte Anteile von Quellen aufweisen, die außerhalb der Abbildungsebene liegen. Die Rohbilder können durch Verschieben und Aufsummieren oder Bildentfaltung zu einem im Signal verbesserten Bild der Struktur verrechnet werden.
(EN) A method, an image processing apparatus and a laser scanning microscope for imaging a fluorescent dye-marked structure in a specimen are described, wherein (bothersome) image background has been reduced in relation to methods from the prior art. The invention is distinguished in that the fluorescence signal is detected by a plurality of detector elements arranged in a detection area and in that raw images are calculated from the signals of the detector elements, said raw images having reduced components of sources located outside of the imaging plane in relation to the detector signals. By shifting and summing or image deconvolution, the raw images can be combined by calculation to form a signal-improved image of the structure.
(FR) L'invention concerne un procédé, un appareil de traitement d'image et un microscope à balayage laser pour imager une structure marquée par un colorant fluorescent dans un échantillon, dans lequel un arrière-plan d'image (gênant) a été réduit par rapport aux procédés de l'état de la technique. L'invention est caractérisée en ce que le signal de fluorescence est détecté par une pluralité d'éléments détecteurs disposés dans une zone de détection et en ce que des images brutes sont calculées à partir des signaux des éléments détecteurs, lesdites images brutes ayant des composantes de sources réduites situées à l'extérieur du plan d'imagerie par rapport aux signaux de détecteur. Par décalage et addition ou déconvolution d'image, les images brutes peuvent être combinées par calcul pour former une image à signal amélioré de la structure.
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